Skin and soft tissue defect repair method by making use of own dermal fibroblast and umbilical cord mesenchymal stem cell

A technology of fibroblasts and stem cells, applied in the field of biological tissue engineering, can solve problems such as limitations and cannot guarantee long-term therapeutic effects

Inactive Publication Date: 2013-05-08
北京清美联创干细胞科技有限公司
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Problems solved by technology

Apparently, although native processed collagen eliminates the immunogenicity of bovine collagen, it does not guarantee long-term therapeutic effect like bovine collagen
Also, it is limited to subjects who have undergone surgery
[0007] In conclusion, none of the existing non-bioinjectable materials can fully satisfactorily augment the proximal dermis and soft tissue

Method used

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  • Skin and soft tissue defect repair method by making use of own dermal fibroblast and umbilical cord mesenchymal stem cell
  • Skin and soft tissue defect repair method by making use of own dermal fibroblast and umbilical cord mesenchymal stem cell

Examples

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Embodiment Construction

[0016] Preparation of umbilical cord mesenchymal stem cells:

[0017] Immediately after the fetus was delivered, the umbilical cord was ligated and cut according to the obstetrical routine, the umbilical cord was washed with normal saline, and then disinfected with medical alcohol, and the umbilical cord was stored in the umbilical cord preservation solution at a constant temperature of 2-8°C. Take no less than 4ml of cord blood as a test sample, and another 4ml of cord blood from the placenta for serum collection.

[0018] After the umbilical cord blood and maternal blood test samples pass the test, the umbilical cord in the laboratory is separated into 1-3mm 3 The separation procedure of the umbilical cord is to cut along the inner side of the umbilical cord ligation, add an appropriate amount of normal saline to wash until the surface is free of blood stains, then cut the umbilical cord into small sections of 2-3cm, cut along the umbilical cord veins and strip the veins and...

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Abstract

The present invention relates to a method for repairing subcutaneous tissue or dermal defects of patients. The method includes cultivating dermal fibroblasts from the patient living samples, cultivating umbilical cord mesenchymal stem cells of the same animal, mixing the two cells proportionally, and using the preparation essentially without immunogenic protein to repair subcutaneous or dermal tissues. The method can be used to repair wrinkles, skin expansion lines, pitting dense scar, and non-traumatic skin pit.

Description

technical field [0001] The invention relates to a method for repairing skin and soft tissue defects by using self-dermal fibroblasts and umbilical cord mesenchymal stem cells, belonging to the field of biological tissue engineering. Background technique [0002] Injection of a material (i.e. injection) into the body, especially the face, to obtain cosmetic effects dates back to the end of the nineteenth century. For example, correction of contour defects by injection of paraffin was accepted short-term before World War I. However, the existence of complications and the unsatisfactory long-term effect made this practice abandoned. Coincidentally the availability of injectable silicones has allowed this process to repeat itself from the 1960s onwards. Special medical grade silicone solution. Local and systemic reactions to silicones, migration of injectables, and local tissue disruption have limited the use of silicone injection methods. Although the original proponents of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38C12N5/071C12N5/0775A61M5/178
Inventor 田杰
Owner 北京清美联创干细胞科技有限公司
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