Application of molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial in glutathione assay

A technology of glutathione and nanomaterials, which is applied in the field of analysis and detection, can solve the problems that the instrument is not easy to carry, and it is difficult to realize mobile detection, and achieve the effect of easy portability, low cost and simple materials

Active Publication Date: 2018-03-23
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current common analysis methods have the disadvantages that the instrument is not easy to carry, and it is difficult to realize mobile detection, etc.

Method used

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  • Application of molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial in glutathione assay
  • Application of molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial in glutathione assay
  • Application of molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial in glutathione assay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1, NaGdF modified by silicomolybdic acid 4 : Yb, Er detect the concentration of glutathione aqueous solution:

[0066] 1) Drawing of standard curve: 200μL of 250nM, 500nM, 1μM, 2μM, 4μM, 8μM, 16μM, 32μM, 64μM, 125μM, 250μM, 500μM, 1mM, 2mM, 3mM, 4mM, 5mM, 6mM, 7mM, 8mM , 9mM, 10mM, 11mM, 12mM, 13mM, 14mM, 15mM, 16mM glutathione aqueous solution and 800μL concentration is 200μg mL -1 Silicomolybdic acid-modified NaLuF 4 : Yb, Er aqueous solution is uniformly mixed, after standing at room temperature (25 ℃) for 10 minutes, the reaction is complete, the fluorescence spectrum of the mixture is measured under the irradiation of 980nm near-infrared light, the linear spectrum of fluorescence is obtained by processing the data, and glutathione is obtained Standard curve of aqueous solution concentration and fluorescence intensity, such as image 3 shown. from image 3 It can be known that the standard spectrum has good linearity in the concentration range of 125 μ...

Embodiment 2

[0068] Embodiment 2, NaLuF modified with silicomolybdic acid 4 :Nd detects the concentration of glutathione aqueous solution:

[0069] 1) Drawing of standard curve: 200μL of 250nM, 500nM, 1μM, 2μM, 4μM, 8μM, 16μM, 32μM, 64μM, 125μM, 250μM, 500μM, 1mM, 2mM, 3mM, 4mM, 5mM, 6mM, 7mM, 8mM , 9mM, 10mM, 11mM, 12mM, 13mM, 14mM, 15mM, 16mM glutathione aqueous solution and 800μL concentration is 200μg mL -1 Silicomolybdic acid-modified NaLuF 4 : Nd aqueous solution is uniformly mixed, and after standing for 10 minutes at room temperature (25° C.), the reaction is complete. Measure the fluorescence spectrum of the mixed solution under 808nm near-infrared light irradiation, process the data to obtain the linear spectrum of fluorescence, and obtain the glutathione aqueous solution. A standard curve of concentration versus fluorescence intensity, such as Image 6 shown. from Image 6 It can be known that the standard spectrum has good linearity in the concentration range of 1mM-16mM, ...

Embodiment 3

[0071] Embodiment 3, NaYF modified by phosphomolybdic acid 4 : Yb, Tm detect the concentration of glutathione aqueous solution:

[0072] 1) Drawing of standard curve: 200μL of 250nM, 500nM, 1μM, 2μM, 4μM, 8μM, 16μM, 32μM, 64μM, 125μM, 250μM, 500μM, 1mM, 2mM, 3mM, 4mM, 5mM, 6mM, 7mM, 8mM , 9mM, 10mM, 11mM, 12mM, 13mM, 14mM, 15mM, 16mM glutathione aqueous solution and 800μL concentration is 200μg mL -1 Phosphomolybdic acid-modified NaYF 4 : Yb, Tm aqueous solution is evenly mixed, after standing at room temperature (25°C) for 10 minutes, the reaction is complete, the fluorescence spectrum of the mixture is measured under the irradiation of 980nm near-infrared light, the linear spectrum of fluorescence is obtained by processing the data, and glutathione is obtained Standard curve of aqueous solution concentration and fluorescence intensity, such as image 3 shown. from image 3 It can be known that the standard spectrum has good linearity in the concentration range of 500nM-...

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Abstract

The invention discloses application of a molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial in glutathione assay. The method comprises the following steps: a series of glutathione solutions with normal concentrations respectively react with the molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial, so that a series of mixed liquors are obtained. Under the irradiation of near-infrared light, the changes of fluorescence intensities are detected; after a standard curve of fluorescence and glutathione concentrations is drawn, glutathione solution with an unknown concentration reacts with the molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial, so that a mixed liquor is obtained; under the irradiation of near-infrared light, the change of fluorescence intensity is detected and compared with the standard curve, and thereby the concentration of the glutathione solution is obtained. The method can quickly, sensitively and portably carry out quantitative assay on the glutathione, such as the quantitative assay of food, medicines, living samples and the like, moreover, the method is easy to operate, the linear effect is good, the limit of detection is low, the cost is low, instruments are convenient to carry, and movable assay can be implemented.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to the application of a molybdenum-based heteropolyacid-modified rare earth fluorescent nanometer material in the detection of glutathione. Background technique [0002] Analytical detection technology is the use of specified methods to test the specified technical performance indicators of test gases, liquids or solids. It is suitable for quality assessment in various industries, such as environment, food safety, water quality, etc. Glutathione is an important biological small molecule with strong reducibility and participates in various biological processes. At present, common glutathione analysis methods include Raman spectroscopy detection and electrochemical detection. However, the current common analysis methods have the disadvantages that the instrument is not easy to carry, and it is difficult to realize mobile detection. Contents of the invent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 周晶刘瑜鑫贾琪郭权炜
Owner CAPITAL NORMAL UNIVERSITY
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