45results about How to "Efficient shooting" patented technology

The invention relates to an image shooting method and device, and belongs to the technical field of image shooting. The method includes the steps of: obtaining a framing image in a shooting framing process; analyzing composition of the framing image; and when the composition of the framing image satisfies composition optimization conditions, optimizing the composition of the framing image. The image shooting method provided by the invention solves the problem that in related technologies, optimization of the composition of an image that is shot in a later period is complicated in operation and needs to cost much time and energy; and the method realizes optimization of the composition of the framing image in the shooting framing process, thereby directly obtaining a composition-optimized high-quality image during shooting, and later processing is not needed.

This invention relates to a high definition shooting method of digital camera. The digital camera includes the zoom lens, the focalizing lens, the aperture, the charge coupled device, the digital signal processing part, the decoder, the servo part, the microprocessor, the storage device, the memorizer, and the liquid crystal display. The mentioned high definition shooting method includes: move the position of the zoom lens from the far-angle ends to the direction of the wide-angle ends, and shoot several video frames meantime, then casually store them in the memorizer or storage device; synthesize the several casually stored video frames to produce video frames with high definition, then register and store them in the storage device. This invention can make the digital camera with zoom lens shoot high definition video frames with high efficiency by using the zoom lens even without using charge coupled device with high pels.

The invention discloses a sgRNA targeting sequence specifically targeting a mouse Krt14 gene and application thereof, and belongs to the technical fields of medical genetics and molecular biology. A nucleotide sequence corresponding to sgRNA is any one of four sequences of SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The invention further discloses a method for editing the mouse Krt14 gene by using the sgRNA targeting sequencing specifically targeting the mouse Krt14 gene. The sgRNA targeting sequence can mediate the efficient cleavage of target DNA by Cas9 protein, and then be usedfor editing the mouse Krt14 gene to affect the function of protein encoded by the mouse Krt14 gene. The sgRNA targeting sequence can achieve efficient targeting by a CRISPR / Cas9 system, and the efficiency is 70%, 90%, 65% and 100% separately.

The invention provides a cutting apparatus which can effectively photograph a cutting groove formed in a processed object. The cutting apparatus (1) can measure the groove width of the cutting groove(G) it forms the high pressure air layer (100) and it blows and it blows the cutter water remaining in the upper side (Wa) or the cutting groove (G) of the plate shape (W) with the whirlpool airstream (101) and therefore more correct including the air layer formation means (33), is one circularity air around the optical axis of the objective lens (310) (34) the imaging device (30) taking a picture of the plate shape (W) which maintains with the sustaining table (10) this is included, the objective lens (310) in the imaging time, of the cutting groove (G) the air introducing hole (35) introducing air to (34) to the circularity air, the guide plate (36), and the reverse transfer phase cone fluid path (37) are included, and the whirlpool airstream (101) among the plate shape (W). As to the guide plate (36), air is swiveled in a specific direction to the circularity air in (34) and creating the whirlpool airstream. The reverse transfer phase cone fluid path (37) is axlediameter measured to the downward to the circularity air from (34).

The invention provides a processing method and a processing device for synthesizing a smiley face, wherein, the method comprises the following steps of: extracting a plurality of frames of images froma buffer queue and selecting a frame of images with a large number of smiley faces as an original image; identifying the non-smiley face object as the target object in the original image, and selecting the image whose target object is smiley face state as the target image in the multi-frame image; processing the target image according to the brightness value of the original image; synthesizing the original image and the target image to make the target object present a smiling face state. By using the method of the invention, the device and the system can efficiently take photographs of all the people who are in the best smiling face state, thereby improving the probability of taking a smiling face as a target object, and improving the final imaging effect of the electronic equipment.

The invention relates to the field of biotechnology, and in particular, relates to a method for knocking out a pig GOT1 gene by a CRISPR / Cas9 system. The invention provides an sgRNA specifically targeting to the pig GOT1 gene, a CRISPR / Cas9 gene knock-out vector containing the sgRNA, a method for knocking out the pig GOT1 gene by the vector, and a detection method for the pig GOT1 gene. The CRISPR / Cas9 knockout vector provided by the invention can achieve high efficiency of the pig GOT1 gene knockout, significantly improve the construction efficiency of GOT1 gene knockout cells and GOT1 gene knockout pigs, and provide an effective method and basis for the function research and application of the pig GOT1 gene.

The invention discloses a substation equipment infrared thermal image shooting navigation method and device. The method is characterized is that, to begin with, a shooting task package is input into a thermal imager, wherein the shooting task package at least comprises interval names, interval models and interval coordinate information of a substation to be shot; a region where a shooting person locates is judged according to the position coordinates of the thermal imager; when the shooting person enters a danger zone, the thermal imager gives a warning signal for reminding of entering the danger zone and reminds the shooting person to leave the danger zone; when the shooting person enters a work zone, the thermal imager gives a reminding signal for reminding of entering the work zone, and reminds the shooting person that infrared thermal image shooting can be carried out; and after the shooting person enters the work zone, the thermal imager judges the position of the shooting person in an interval, reminds the shooting person to carry out shooting, and automatically associates and records the names of shot devices in shot infrared thermal images. The navigation method and device is specially developed for substation equipment infrared thermal image shooting, thereby reducing burden of task package producers and shooting persons, improving shooting safety and efficiency, saving cost and facilitating big data archiving and cloud computing of the infrared thermal images.

The invention discloses a resin optical filter film crack detection device and method. The device comprises a bearing device, a light source device, an image acquisition device and an image processingand analyzing system. The bearing device is used for bearing a to-be-detected optical filter; the light source device is arranged below the bearing device and is used for illuminating the optical filter; the image acquisition device is arranged above the bearing device, is arranged on the same vertical plane as the light source device, and is used for acquiring and outputting image information ofthe optical filter; and the image processing and analyzing system is used for receiving and processing the image information of the optical filter outputted by the image acquisition device and judging whether the optical filter has a film crack defect or not. According to the invention, the device and method can efficiently and comprehensively shoot and detect the film cracks of the resin opticalfilter, achieve the sorting of the optical filter with the film cracks, iron out the defect that film cracks exist in optical filter products leaving factories, and guarantees the stability and the uniformity of the quality of the optical filters.

The invention relates to a method for measuring ultraviolet ray filtering of different liquids through sunshine ultraviolet spectrum. The method comprises steps of: (1) using sunshine as a light source, permeating through an empty quartz colorimetric ware and scanning at 280 nm-400 nm and recording an ultraviolet spectrum; (2) using sunshine as a light source, permeating through a quartz colorimetric ware containing a known liquid, scanning at 280 nm-400 nm and recording an ultraviolet spectrum; (3) comparing an ultra violet radiation intensity obtained from step (2) and an ultra violet radiation intensity obtained from step (1) and establishing a corresponding UVA ultraviolet ray transmissivity spectrum; (4) comparing the UVA transmissivity spectrum of the known liquid and determining capability of filtering UVA of the liquid. The invention uses sunlight as an easily available ultraviolet source on the ground, is at low cost and safe, can carry out rapid and efficient detection with high accuracy, and has strong applicability and good application prospect.

The invention relates to a transcriptional activator subsample effector nuclease, and coding gene and application thereof. The transcriptional activator subsample effector nuclease comprises three pairs of short peptides, wherein the three pairs of short peptides are respectively a first pair of short peptides, a second pair of short peptides and a third pair of short peptides; the first pair of short chain peptides identifies DNA sequences of SEQIDNO.1 and SEQIDNO.2; the second pair of short peptides identifies DNA sequences of SEQIDNO.3 and SEQIDNO.4; the third pair of short peptides identifies DNA sequences of SEQIDNO.5 and SEQIDNO.6. The transcriptional activator subsample effector obtained by establishing the three pairs of short peptides can respectively and specifically identify two adjacent nucleotides near gene seed sequences of human miRNAs: miR-133b, miR-141 and miR-488, and by utilizing the transcriptional activator subsample effector nuclease obtained by establishing the three pairs of transcriptional activator subsample effectors, accurate and efficient targeting can be performed on the genes of human miRNAs: miR-133b, miR-141 and miR-488.

The invention provides a pair of short peptides, a pair of transcription activator-like effectors and a pair of DNA vectors. The short peptides of the invention can realize accurate and efficient targeting of the Tp53 gene of a rat so as to rapidly acquire a Tp53-knockout rat. The invention also provides a method for knocking out the Tp53 gene and a construction method for a Tp53-knockout animal model. The acquired Tp53-knockout rat can be used for research related to tumors, including molecular biology study of tumors, research on neoplastic diseases, examination of carcinogenicity and the like, and has good application prospects.

The invention discloses a sgRNA targeting sequence specifically targeting the mouse G6pc gene and application thereof, and belongs to the technical field of medical genetics and molecular biology. Thenucleotide sequence corresponding to the sgRNA contains the sequence as shown in SEQ ID NO. 2. The invention also discloses a method for editing the mouse G6pc gene by using the above sgRNA targetingsequence specifically targeting the mouse G6pc gene. The sgRNA targeting sequence of the invention can mediate the Cas9 protein and efficiently cut target DNA, and further can be used for editing themouse G6pc gene to affect the function of mouse G6pc gene encoding protein. The sgRNA targeting sequence can efficiently realize targeting by a CRISPR / Cas9 system with an efficiency of 100%.

The invention discloses an air purifier used in a workshop with flying dust and belongs to the field of environmental protection equipment. A contaminant discharging pipe and a blowing pipe each communicate with an air supply pipe and are each arranged to be close to a filter screen. The connection positions, on the surface of the air supply pipe, of the contaminant discharging pipe and the blowing pipe are opposite. An air blower is arranged at the tail end of the blowing pipe. The contaminant discharging pipe is connected to the outside of the workshop. A beating mechanism used for beating the filter screen is arranged at the air outlet end of the air supply pipe. The beating mechanism comprises elastic pieces, a motor, a transmission shaft and a shifting piece set, wherein the transmission shaft rotate along with the motor coaxially, and the shifting piece set is located on the transmission shaft. The transmission shaft is arranged to be in parallel with the filter screen. The elastic pieces are vertically arranged at the bottom side, close to the air outlet end, of an exhaust fan. The shifting piece set is arranged at the positions, corresponding to the elastic pieces, on the transmission shaft. When the shifting piece set rotates along with the transmission shaft, by means of the shifting piece set, the elastic pieces are deformed intermittently toward the ends far away from the filter screen.

The embodiment of the invention discloses a shooting method and device, electronic equipment and a storage medium. The method comprises the following steps: determining at least two to-be-shot target objects so as to obtain to-be-shot objects in a game picture; detecting the orientation information of the target objects by determining the orientation information of the target parts of the at least two target objects; the orientation information of the at least two target objects is acquired, and shooting of the at least two target objects is triggered according to the orientation information, so that shooting of the game picture is triggered based on the orientation information of each target object, high-efficiency shooting of the game picture is realized, accurate shooting of the key game picture is realized, timely recording of the highlight moment of a user is realized, and the user experience is improved.

The embodiment of the invention discloses a shooting method and device, electronic equipment and a storage medium. The method comprises the following steps: determining at least two target objects to be shot; determining orientation information of the target parts of the at least two target objects, and determining a target triggering area according to the orientation information of each target object; and triggering to shoot the at least two target objects based on the target triggering area. According to the technical scheme, the orientation information of the target object is determined, so that the target triggering area of the target objects is further determined, the target triggering area is used as the shooting triggering condition, the game picture is shot, the high-efficiency shooting of the game picture is achieved, the accurate shooting of the key game picture is realized, so that timely recording of the highlight moment of the user is realized, and furthermore, the experience feeling of the user is improved.

The invention provides a pair of short peptides, a pair of transcription activator-like effectors and a pair of DNA vectors. The short peptides of the invention can realize accurate and efficient targeting of the Tp53 gene of a rat so as to rapidly acquire a Tp53-knockout rat. The invention also provides a method for knocking out the Tp53 gene and a construction method for a Tp53-knockout animal model. The acquired Tp53-knockout rat can be used for research related to tumors, including molecular biology study of tumors, research on neoplastic diseases, examination of carcinogenicity and the like, and has good application prospects.

The invention provides a device used for shooting a low-speed wire cut electrical discharge machining discharge channel. The device comprises a bottom plate, a junction plate, connection plates, Z-shaped iron parts and guide columns. The junction plate and the bottom plate are spliced and form a bottom plane together. The connection plates are arranged on the lower surface of the bottom plate andthe lower surface of the junction plate and are used for fixing the bottom plate and the junction plate. One end of each Z-shaped iron part is fixedly connected with the bottom plate through bolts, and the other end of each Z-shaped iron part is fixedly connected with the corresponding connection plate through set screws. The guide columns are arranged at the positions of four vertex angles of thebottom plane through countersunk bolts. The connection portions of the bottom plate and the junction plate are each arranged into a stair shape for splicing. A water leakage groove is further formedin the bottom plate. An electrode wire penetrates through the water leakage groove. A flow limiting plate used for covering the water leakage groove is further arranged on the junction plate. The junction plate and the connection plates are fastened and connected through two bolt and nut connection pieces.

The invention provides a pair of short peptides, a pair of transcription activator-like effectors and a pair of DNA vectors. The short peptides of the invention can realize accurate and efficient targeting of the Il2rg gene of a rat so as to rapidly acquire an Il2rg-knockout rat. The invention also provides a method for knocking out the Il2rg gene and a construction method for an Il2rg-knockout animal model. The acquired Il2rg-knockout rat can be used for research on allotransplantation of tumors, development of humanized rats and research on autoimmune and infectious diseases, and has good application prospects.

The invention relates to the field of biotechnology, in particular to a method for knocking out the pig GOT1 gene by using the CRISPR / Cas9 system. The invention provides an sgRNA specifically targeting the pig GOT1 gene, a CRISPR / Cas9 gene knockout vector comprising the sgRNA, a method for knocking out the pig GOT1 gene by using the vector, and a detection method for the pig GOT1 gene. The CRISPR / Cas9 knockout vector provided by the present invention can achieve a higher knockout efficiency of the pig GOT1 gene, significantly improve the construction efficiency of GOT1 knockout cells and GOT1 knockout pigs, and is useful for the functional research and application of the pig GOT1 gene Effective methods and foundations are provided.

The invention relates to a folding bracket for medical radioscopy inspection and aims to solve the problem that a conventional folding bracket for medical radioscopy inspection has no function of adjusting heights and rotation and lying angles. The folding bracket for medical radioscopy inspection comprises a seat plate, wherein the lower part of the seat plate is connected with a rotating disc through a hydraulic lifting column; the rotating disc is fixed on a bottom disc through a flat push bearing; the rotating disc is provided with a locking sliding block; the left side of the seat plate is connected with a back plate through a first rotating mechanism; and the right side of the seat plate is connected with a leg plate through a second rotating mechanism.

To provide a cutting device capable of efficiently photographing cutting grooves formed in a workpiece. The cutting device (1) has an imaging unit (30) for photographing the plate-shaped workpiece (W) held by the holding table (10). ) between the bottom (321) of the plate-shaped workpiece and the air layer forming unit (33) of the air layer, the air layer forming unit (33) has: an annular air path (34), which takes the optical axis of the objective lens (310) as the center; air inlet (35), which introduces air to the annular air path (34); guide plate (36), which makes the air swirl in one direction in the annular air path (34) to generate a vortex flow; and The reverse annular conical flow path (37), its diameter shrinks downward from the annular air path (34), when shooting the cutting groove (G), a vortex air flow (101 ) of the high-pressure air layer (100), the cutting water remaining on the upper surface (Wa) of the plate-shaped workpiece and the cutting groove is blown away by the vortex air flow (101), so that the groove width of the cutting groove can be measured more accurately.

The present invention provides a tomographic imaging device, which comprises: a scanning frame (3), which is tilted so that the central axis (9a) of the opening (9) is tilted in such a way that the opening (9) faces obliquely downward; a chair (5), It is arranged obliquely below the scanning frame (3), and the surface for obliquely placing the object (M) is inclined so that the central axis (9a) of the opening (9) is aligned with the object (M) The body axis (A) is parallel; the chair moving mechanism (7) drives at least one of the scanning frame (3) and the chair (5), and moves the subject (M) to the opening (9) The central axis (9a) is inserted into the opening (9) so as to be parallel. Therefore, the subject (M) can be imaged safely and efficiently with a comfortable posture.

The invention discloses an sgRNA guide sequence of a specific targeting mouse Gaa gene and an application thereof, which belong to the technical field of medical genetics and molecular biology. A nucleotide sequence corresponding to the sgRNA is any one of SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The invention also discloses a method for editing the mouse Gaa gene by using the sgRNA guide sequence of the specific targeting mouse Gaa gene. The sgRNA guide sequence disclosed by the invention can mediate Cas9 protein to efficiently cut target DNA, so that the sgRNA guide sequence is used forediting the mouse Gaa gene to influence the function of encoding protein of the mouse Gaa gene. According to the sgRNA guide sequence, efficient targeting can be achieved through a CRISPR / Cas9 system,and the efficiency is 100%.

The invention discloses a single guide RNA (sgRNA) guiding sequence specifically targeting a mouse Galt gene and application of the sgRNA guiding sequence, and belongs to the technical field of medical genetics and molecular biology. The nucleotide sequence corresponding to sgRNA includes any one of the sequences shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The invention further discloses amethod for editing the mouse Galt gene through the sgRNA guiding sequence specifically targeting the mouse Galt gene. The sgRNA guiding sequence can mediate a Cas9 protein to cut target DNA efficiently, and thus the sgRNA guiding sequence is used for editing the mouse Galt gene to affect the protein coding function of the mouse Galt gene. The sgRNA guiding sequence can mediate a CRISPR / Cas9 systemto achieve efficient target shooting with the efficiency of 100%.

The invention, which belongs to the technical field of medical genetics and molecular biology, discloses an sgRNA guide sequence of a specific targeting mouse Idua gene and application thereof. The nucleotide sequence corresponding to the sgRNA is any one of SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. In addition, the invention also discloses a method for editing the mouse Idua gene by using thesgRNA guide sequence of the specific target mouse Idua gene. According to the sgRNA guide sequence disclosed by the invention, Cas9 protein can be mediated and the target DNA can be cut efficiently, so that the mouse Idua gene is edited to affect the protein encoding function of the mouse Idua gene. The efficient targeting can be realized by using the CRISPR / Cas9 system and the efficiencies are respectively 100%, 83.33% and 100%.

The invention discloses a portable and rechargeable camera shooting equipment support for an operation. The camera shooting equipment support comprises a spiral track, a supporting seat is fixedly connected to the upper end of the spiral track, a connecting sleeve is fixedly connected to the middle of a supporting rod, the connecting sleeve is movably connected with a threaded cylinder through threads, the lower end of a fixing rod is fixedly connected with a fixing plate, unthreaded rods are fixedly connected to the front side and the rear side of the upper side of the fixing plate, a rotating plate is fixedly connected to the upper end of the threaded cylinder, traveling racks are fixedly connected to the left side and the right side of the inner wall of the spiral track, a first connecting rod is arranged on the outer side of a connecting side plate, traveling gears are in meshed connection with the traveling racks, a moving seat is arranged on the inner side of the connecting sideplate, a lifting rack is fixedly connected to one side, close to the moving seat, of the connecting side plate, a lifting gear is in meshed connection with the lifting rack, and a connecting seat is arranged below the moving seat. The camera shooting equipment support has the advantages of being small in occupied area, convenient to move for the camera shooting, wide in shooting range and the like.

The invention discloses a portable and rechargeable camera shooting equipment support for an operation. The camera shooting equipment support comprises a spiral track, a supporting seat is fixedly connected to the upper end of the spiral track, a connecting sleeve is fixedly connected to the middle of a supporting rod, the connecting sleeve is movably connected with a threaded cylinder through threads, the lower end of a fixing rod is fixedly connected with a fixing plate, unthreaded rods are fixedly connected to the front side and the rear side of the upper side of the fixing plate, a rotating plate is fixedly connected to the upper end of the threaded cylinder, traveling racks are fixedly connected to the left side and the right side of the inner wall of the spiral track, a first connecting rod is arranged on the outer side of a connecting side plate, traveling gears are in meshed connection with the traveling racks, a moving seat is arranged on the inner side of the connecting sideplate, a lifting rack is fixedly connected to one side, close to the moving seat, of the connecting side plate, a lifting gear is in meshed connection with the lifting rack, and a connecting seat is arranged below the moving seat. The camera shooting equipment support has the advantages of being small in occupied area, convenient to move for the camera shooting, wide in shooting range and the like.

The invention discloses an sgRNA guide sequence of a specific targeting mouse Tyr gene and an application thereof, which belong to the technical field of medical genetics and molecular biology. A nucleotide sequence corresponding to the sgRNA is any one of SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The invention also discloses a method for editing the mouse Tyr gene by utilizing the sgRNA guidesequence of the specific targeting mouse Tyr gene. The sgRNA guide sequence disclosed by the invention can mediate Cas9 protein to efficiently cut target DNA, so that the sgRNA guide sequence is usedfor editing the Tyr gene of a mouse to influence the function of the Tyr gene encoding protein of the mouse. According to the sgRNA guide sequence, efficient targeting can be achieved through a CRISPR / Cas9 system, and the efficiency is 100%.