Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of sgRNA guide sequence specifically targeting mouse galt gene and its application

A kind of specific, mouse technology, applied in the fields of medical genetics and molecular biology, can solve the problem of no sgRNA guide sequence knockout, and achieve the effect of high-efficiency screening, increase in quantity, and high concentration

Active Publication Date: 2021-02-02
GUILIN MEDICAL UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no sgRNA guide sequence for the mouse Galt gene and a method for knocking out the gene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of sgRNA guide sequence specifically targeting mouse galt gene and its application
  • A kind of sgRNA guide sequence specifically targeting mouse galt gene and its application
  • A kind of sgRNA guide sequence specifically targeting mouse galt gene and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0084] The principles and features of the present invention will be described below in conjunction with specific drawings, and the examples given are only used to explain the present invention, not to limit the scope of the present invention.

[0085] 1. Screening of human GALT gene pathogenic mutation sites, and confirming that human GALT gene c.904+1G is a pseudo-mutation site.

[0086] Type I galactosemia is caused by mutations in the GALT gene. First, the inactivation mutation of the human GALT gene was screened using the ExAC database, and a total of 19 mutation sites were screened (as shown in Table 1 and figure 1 shown); in order to more extensively screen the pathogenic mutations of GALT, the present invention also utilizes the OMIM online database to retrieve 17 kinds of GALT mutations (as shown in Table 2); then, the present invention utilizes the ClinVar database to retrieve splicing site mutations The disease-causing conditions (as shown in Table 3). In view of th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a single guide RNA (sgRNA) guiding sequence specifically targeting a mouse Galt gene and application of the sgRNA guiding sequence, and belongs to the technical field of medical genetics and molecular biology. The nucleotide sequence corresponding to sgRNA includes any one of the sequences shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The invention further discloses amethod for editing the mouse Galt gene through the sgRNA guiding sequence specifically targeting the mouse Galt gene. The sgRNA guiding sequence can mediate a Cas9 protein to cut target DNA efficiently, and thus the sgRNA guiding sequence is used for editing the mouse Galt gene to affect the protein coding function of the mouse Galt gene. The sgRNA guiding sequence can mediate a CRISPR / Cas9 systemto achieve efficient target shooting with the efficiency of 100%.

Description

technical field [0001] The invention relates to a sgRNA guide sequence specifically targeting mouse Galt gene and application thereof, belonging to the technical fields of medical genetics and molecular biology. Background technique [0002] The CRISPR / Cas system was first discovered in bacteria, and it performs acquired immune functions in eubacteria and archaea, which can resist the invasion of foreign viruses and plasmids. People have modified the CRISPR / Cas system of the microorganism itself by means of genetic engineering, thereby creating a targeting system that can be widely used in gene editing of higher organisms, the CRISPR / Cas9 system. Since the system came out in 2012, it has injected strong impetus into life science and biomedical research, and has become a research hotspot in recent years. Since 2013, researchers have successfully edited the genome in mammalian cells by using the DNA binding activity and endonuclease activity of CRISPR / Cas9. In the CRISPR / Cas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/85C12N15/90C12N5/10
CPCC07K14/47C12N15/113C12N15/907C12N2310/20
Inventor 于鸿浩岳鹏鹏付灿郭俊璠李勇
Owner GUILIN MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com