2 results about "Knockout rat" patented technology

The application discloses a method for preparing GHR humanized animal cells by using a CRISPR system, which comprises the following steps: designing and synthesizing sgRNA; mixing the sgRNA with a Cas9 protein to form a CRISPR-Cas9 complex; constructing donor DNA containing coding sequences of a human GHR extracellular region and a rat GHR intracellular region; injecting the CRISPR-Cas9 complex and the donor DNA into the cytoplasm or nucleus of a rat fertilized ovum to knockout the coding sequence of the extracellular region of the rat-derived GHR gene and simultaneously insert the coding sequence of the extracellular region of the human-derived GHR gene into the genome, so that the GHR humanized animal cells are obtained. The application further discloses a method for constructing a GHR humanized rat model by using the CRISPR system, and application of the GHR humanized animal cells and the GHR humanized rat model obtained by the above method in drug screening, drug development or efficacy evaluation. The application can more accurately simulate the interaction between the human GHR and growth hormone and other ligands, improves the accuracy and reliability of research, and has the advantages of simple gene editing operation, high editing efficiency, strong specificity, low off-target effect and wide application range.

The invention belongs to the technical field of medical biological research, and particularly relates to construction and evaluation of a chronic obstructive pulmonary epithelial cell senescence model. A method for constructing a chronic obstructive pulmonary epithelial cell senescence model comprises the following steps: exposing tobacco smoke after 6-8 weeks of Muc1 gene whole-body knockout mice and / or exposing tobacco smoke after 6-8 weeks of Muc1 whole-body knockout rats and / or injecting tamoxifen into abdominal cavities of the mice when the Muc1 type II alveolar epithelial cell knockout mice are 6-8 weeks old, and then exposing tobacco smoke every one week. According to the invention, a series of evaluations are carried out on lung tissue pathological changes, lung epithelial cell changes and other indexes of the constructed model product, and the success of the construction of the chronic obstructive pulmonary pulmonary epithelial cell senescence model is jointly proved.