Rapid three-dimensional microscopic imaging system

Abstract

The invention discloses a rapid three-dimensional microscopic imaging system. The system includes a microscope, a field iris diaphragm, a narrow-band optical filter, a beam-splitting grating, a multiple focal-length lens array, a micro-lens array and an image sensor. The microscope amplifies a microscopic sample and images the microscopic sample to an image-plane extraction opening; the field iris diaphragm is used for restraining a post-imaging field range; the narrow-band optical filter is used for conducting narrow-band optical filtering on information from the sample; the beam-splitting grating is used for conducting beam splitting on microscopic output images at two-dimensional space, wherein the beam splitting is conducted after the microscopic output images go through a first-stage 4f system; the multiple focal-length lens array is used for adding different phase modulations into various light beams, so that images with different depths are obtained on a post-lens rear focal plane; the micro-lens array is used for modulating light rays at different angles to different special positions behind micro lenses, wherein the modulating is conducted after the light rays at the different angles go through the beam-splitting grating and the multiple focal-length lens array; the image sensor is used for recording sample images which are modulated by preceding stages. According to the system, the multi-depth synchronous imaging can be achieved at the single imaging speed of normal wide-field microscopes, the imaging is fast, the image resolution is high, and the system can be used for living samples; meanwhile, the system is simple in structure, and the imaging depth interval is convenient to adjust.

Description

technical field [0001] The invention relates to the technical field of computational photography, in particular to a fast three-dimensional microscopic imaging system. Background technique [0002] Life science and material science research put forward higher requirements for 3D microscopic imaging. At present, most microscopic three-dimensional imaging uses confocal microscopes or two-photon and multiphoton microscopes to scan and collect fluorescent samples point by point to form images at different depths. However, these methods have two disadvantages. First, the required excitation light is strong, which is likely to cause fluorescence bleaching, and strong light irradiation may damage sample cells or tissue structures; second, imaging a specific depth of a fluorescent sample requires point-by-point scanning, which is inefficient in time. Therefore, the above methods are not suitable for photosensitive fluorescent samples and biological samples, and the imaging speed i...

AI Technical Summary

Problems solved by technology

However, the use of wide-field microscopy to obtain fluorescence images has the following problems: the excitation light not only excites the fluorescence signal at the focal plane of the objective lens, but also excites the fluorescence signal at the non-focal plane. Fluorescence intensity and non-focal plane fluorescence intensity and noise superposition, and the image resolution obtained at each depth is lower than that of the prev...

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