Method for performing stepped detection on hydroxyl radical concentration of solution

A free radical and hydroxyl technology, applied in the field of analysis and detection, can solve the problems of inaccurate and effective quantitative detection, poor sensitivity, slow detection speed, etc., and achieve the effect of improving the linear detection range, reducing the detection limit, and simple materials

Inactive Publication Date: 2017-06-13
CAPITAL NORMAL UNIVERSITY
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Problems solved by technology

However, there are many defects in the current common analysis methods, such as: slow detectio

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  • Method for performing stepped detection on hydroxyl radical concentration of solution
  • Method for performing stepped detection on hydroxyl radical concentration of solution
  • Method for performing stepped detection on hydroxyl radical concentration of solution

Examples

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Embodiment

[0062] Example, indocyanine green modified NaLuF 4 : Yb, Er rare earth nanomaterials to detect the concentration of hydroxyl radical solution:

[0063] 1) Drawing of standard curve: 300 μL of 125nM, 250nM, 375nM, 500nM, 750nM, 1μM, 1.5μM, 2μM of hydroxyl radical solution and 700μL of 1mM indocyanine green modified NaLuF 4 : The aqueous solution of Yb and Er rare earth nanomaterials is evenly mixed, and after standing at 25°C for 10 minutes, measure the UV-visible absorbance of the mixture, process the data to obtain the UV-visible absorbance, and obtain the standard curve of concentration and absorbance, as shown in figure 1 shown. The detection range is 125nM-2μM, and the lowest detection limit can reach 4pM.

[0064] Separately mix 300 μL of 125pM, 250pM, 500pM, 1nM, 2nM, 4nM, 8nM, 16nM, 32nM, 63nM, 125nM, 250nM hydroxyl radical solution with 700μL, 1mM indocyanine green modified NaLuF 4 : The aqueous solution of Yb and Er rare earth nanomaterials is evenly mixed, and aft...

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Abstract

The invention discloses a method for performing stepped detection on hydroxyl radical concentration of a solution. The method comprises the following steps: 1) modifying a nanometer material containing rare earth elements by using cyanine micromolecular compounds capable of reacting with hydroxyl radical to obtain a functionalized rare earth nanometer material; 2) performing reaction on a series of hydroxyl radical solutions with standard concentration and the functionalized rare earth nanometer material to obtain a series of mixed liquids, measuring the light intensity, the ultraviolet-visible light-absorbing intensity and the photo-thermal heating temperature of the mixed liquid separately, and drawing standard curves of the light intensity, the ultraviolet light-absorbing intensity, the heating temperature and the concentration separately; and 3) performing reaction on a to-be-detected radical solution with unknown concentration and the functionalized rare earth nanometer material to obtain a mixed liquid, determining the detection mode, measuring corresponding data and comparing with the standard curves to know the concentration. The detection limit is effectively reduced and the linear detection range is increased. The method is simple in material and low in instrument price, and can be applied to detection of samples such as food, medicines and living samples.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a method for detecting the concentration of hydroxyl radicals in a solution in a stepwise manner based on functionalized rare earth nanometer materials. Background technique [0002] Hydroxyl free radicals are small molecules with strong oxidative properties produced by the natural metabolism of organisms. Their oxidation-reduction potential is second only to fluorine, and they can affect a variety of biological processes. The abnormal content of hydroxyl radicals in organisms will increase the level of oxidative stress in organisms, and cause damage to single-stranded DNA, denaturation of proteins, etc. Therefore, the detection of hydroxyl radicals has practical significance. At present, common analytical methods include optical detection and electrochemical detection. However, the current common analysis methods have many defects, such as: slow dete...

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Application Information

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IPC IPC(8): G01N21/31G01N21/62G01N25/20
CPCG01N21/31G01N21/62G01N25/20
Inventor 周晶刘瑜鑫郭权炜贾琪
Owner CAPITAL NORMAL UNIVERSITY
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