A Quantitative Analysis Method and Application of Enhanced SERS Signal

A quantitative analysis and signal technology, applied in the field of analysis and testing, can solve problems such as poor repeatability, achieve good reproducibility, improve accuracy, improve precision and sensitivity

Inactive Publication Date: 2017-08-04
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the wet SERS detection is greatly affected by the particle size, shape and aggregation degree of gold or silver sol, so the repeatability is relatively poor; in addition, when a large number of samples need to be analyzed, the wet SERS detection is not as good as the dry SERS detection. Convenient SERS detection

Method used

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  • A Quantitative Analysis Method and Application of Enhanced SERS Signal
  • A Quantitative Analysis Method and Application of Enhanced SERS Signal
  • A Quantitative Analysis Method and Application of Enhanced SERS Signal

Examples

Experimental program
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Embodiment

[0036] Embodiment: conical hole glass plate SERS enhanced substrate and surface enhanced Raman spectroscopy quantitative analysis multiplier effect model (MEM SERS ) combined with the quantitative analysis of 6-mercaptopurine in plasma samples.

[0037] In this example, we first use the ethanol solution of 4-mercaptopyridine to determine the optimum value of the diameter and height of the conical hole on the glass substrate, and then combine the conical hole glass substrate with the optimal diameter and height with the MEM SERS The combination of the model and the accurate quantitative analysis of 6-mercaptopurine in plasma samples by SERS spectrum.

[0038] Reagents and sources used in this example are as follows: 4-mercaptopyridine (96%), 4-mercaptobenzoic acid (90%), 6-mercaptopurine monohydrate (99.5%), 3-aminopropyltriethoxysilane (APTMS, 98%) and silver nitrate (99.9%) were purchased from Aladdin Reagent Co., Ltd.; trisodium citrate dihydrate (analytical pure) was purch...

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Abstract

The invention provides an enhanced SERS (surface enhanced raman scattering) signal quantitative analysis method. The enhanced SERS signal quantitative analysis method comprises the following steps: (1) drilling a conical hole or a cylindrical-conical hole in a substrate, wherein the cylindrical-conical hole is characterized in that a cylindrical hole is connected above the conical hole, and one end of the conical hole is connected with the cylindrical hole; (2) depositing nanoparticles on the surface of the substrate with the hole drilled in the step (1) to obtain an enhanced base; (3) obtaining SERS spectroscopic data of a corrected sample by utilizing an enhanced base and internal standard combined method; (4) building a correction model between the SERS spectroscopic data of the correction sample and to-be-detected material concentration vector c; and (5) obtaining SERS spectrum xtest of a to-be-tested sample by utilizing the enhanced base and internal standard combined method; and calculating concentration of to-be-tested substance in the to-be-tested sample by adopting the correction model. The enhanced SERS signal quantitative analysis method has the effect that the enhancement effect is improved by 5-10 times compared with a planar enhanced base and can be applied to quantitative analysis of trace substance.

Description

technical field [0001] The invention relates to a quantitative analysis method for enhancing SERS signals and its application in quantitative analysis of trace substances, belonging to the field of analysis and testing. Background technique [0002] Surface Enhancement of Raman Scattering (SERS, Surface Enhancement of Raman Scattering) effect refers to the pulling of adsorbed molecules due to the enhancement of the electromagnetic field on the surface or near the surface of the sample in the specially prepared surface or sol of some metal good conductors. The phenomenon that the Raman scattering signal is greatly enhanced compared with the ordinary Raman scattering signal. At present, the SERS mechanism generally accepted in academia can be divided into two categories: electromagnetic effect and chemical effect. Electromagnetic effects are the result of an increase in the electric field strength of the analyte due to the interaction of the metal surface with the excitation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
Inventor 陈增萍陈瑶田中群
Owner HUNAN UNIV
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