Full-field optical blood flow velocity analysis equipment and implementation method thereof

Abstract

The invention discloses full-field optical blood flow velocity analysis equipment which comprises a vibration isolation optical table arranged in a darkroom and a customized container, a light source,an optical fiber coupler, an extension lens, diffusing glass, a support, a fixing frame, a high-speed complementary metal oxide semiconductor camera, a zoom double-center decentered lens and a computer which are arranged on the vibration isolation optical table. The customized container is used for storing biological samples; the light source is connected to the optical fiber coupler through optical fiber; according to the equipment, full-field optical angiography images of live samples are studied, and erythrocyte signals of background tissues are successfully highlighted; combined with image processing techniques such as fuzzy segmentation and skeleton extraction, blood vessels can be accurately segmented out from the full-field optical angiography images; the defect is overcome that laser speckles can only be used for measuring relative velocity of blood flow, and the defect is also overcome that the Doppler method can provide accurate transverse blood flow velocity measurement only in the direction of incident light.

Description

technical field [0001] The invention relates to the technical field of blood flow velocity measurement, in particular to a full-field optical blood flow velocity analysis device and its implementation method. Background technique [0002] At present, some optical imaging technologies have been applied in the field of blood flow velocity measurement, such as laser speckle imaging system and laser Doppler imaging system: laser speckle imaging system is mainly composed of coherent light source and image processing equipment, this technology mainly uses biological tissue The blood flow velocity is obtained by the backscattered light of the biological tissue, and the coherent light is randomly superimposed after being scattered by the biological tissue. The image processing equipment collects and analyzes the random speckle pattern to finally estimate the blood flow velocity; the laser Doppler imaging system is based on multiple The measurement of blood flow velocity is affected ...

AI Technical Summary

Problems solved by technology

[0003] However, when using laser speckle imaging system to measure blood flow velocity, due to environmental conditions (such as light source intensity, illumination angle, imaging object and camera parameters) and scattering processes in biological tissues (such as velocity distribution, static speckle and multiple Speckle) makes the blood flow velocity measured by the laser speckle imaging method a relative velocity rather than an absolute velocity, and there is a large error. Therefore, this method cannot accurately measure the lateral velocity of the blood flow; in addition, many According to the Doppler effect, the blood flow velocity can be accurately obtained only when the incident beam of the laser is parallel to the direction of the blood flow, so the laser Doppler imaging system can only provide accurate radial blood flow velocity measurement along the direction of the incident light. For some flat biological samples, such as chicken embryo, zebrafish and retina, the imaging plane is perpendicular to the direction of incident light, while the movement direction of blood flow is transverse, therefore, laser Doppler imaging systems relying on the Doppler effect Unable to measure lateral velocity of blood flow

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