Method for detecting diarrhea shellfish toxin

A shellfish toxin and detection method technology, applied in the preparation of test samples, fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of low sensitivity, poor accuracy, poor comparability and repeatability, etc., and achieve simple sample extraction, Low matrix effect and good specificity
CN101975768AInactive Publication Date: 2011-02-16SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
Publication Date
2011-02-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting diarrhea shellfish toxin, which comprises the following steps: A) establishing a standard curve of depolymerization of okadaic acid (OA) induced HL-7702 hepatocyte F-actin; B) carrying out the calculation on the concentration of the OA according to the standard curve of the F-actin depolymerization of the HL-7702 hepatocyte, establishing a detection method of the hepatocyte F-actin of the diarrhea shellfish toxin to determine the possible detection limit range; C) selecting one or more of components (STX, DA and YTX) which are possibly coexist with the diarrhea shellfish to act on the HL-7702 hepatocyte, determining the specificity of the method for detecting the OA content by detecting the degree of damaging the polymerizing power of the HL-7702 hepatocyte F-actin by the above components. The detection method of the invention has the advantages: (1) taking cells as experimental subjects to avoid the using of experimental animals and conforming to the rule of 3R; (2) having high sensitivity; (3) having good specificity; (4) having good repeatability; and (5) having simple and convenient sample extraction and low matrix effects.
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Description

technical field

[0001] The invention belongs to the field of biological detection methods, in particular to a detection method for diarrhea shellfish toxin with high sensitivity. Background technique

[0002] Currently, the detection methods for diarrheal shellfish poisoning (DSP) in my country mainly include mouse biological method and enzyme-linked immunosorbent assay (enzyme linked immunosorbent assay, ELISA).

[0003] The detection principle of the mouse biological method: shellfish samples are extracted with acetone and ether, evaporated to dryness under reduced pressure, dissolved in 1% Tween-60 saline and injected into the abdominal cavity of mice, the survival of the mice is observed, and the toxicity is calculated. force.

[0004] The detection principle of ELISA: the basis of the determination is the antigen-antibody reaction, the microwell plate is coated with the capture antibody against the okadaic acid (OA) (the main component of DSP) antibody, and the standar...

Claims

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