Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Paralytic shellfish poisoning (PSP) standard sample and preparation method and application thereof

A standard sample and shellfish toxin technology, applied in the biological field, can solve the problems of high price and no matrix, and achieve the effects of low raw material cost, guaranteed effectiveness, and simple preparation method

Inactive Publication Date: 2011-11-09
王秋艳 +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, PSP standard products are all single-component standard pure products without matrix, and the price is quite expensive. Each component is as high as about 6000 yuan / 100μg. In addition, PSP is a highly toxic substance. There are strict regulations on the use and use of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paralytic shellfish poisoning (PSP) standard sample and preparation method and application thereof
  • Paralytic shellfish poisoning (PSP) standard sample and preparation method and application thereof
  • Paralytic shellfish poisoning (PSP) standard sample and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] Such as figure 2 Shown, the preparation method of paralytic shellfish toxin standard sample of the present invention comprises the following steps: (A) sampling, impurity removal; (B) acidification, homogenate; (C) primary freeze-drying; (D) grinding, sieving (E) freeze-drying again; (F) encapsulation; (G) uniformity and stability inspection; (H) determination of PSP content.

[0029] Specifically, the preparation method of the paralytic shellfish toxin standard sample of the present invention comprises the following steps:

[0030] (A) Take the PSP-positive scallop, wash the shell surface thoroughly with clean water, cut off the adductor muscle, open the shell, rinse the inside with heavy distilled water to remove impurities such as sediment and other foreign objects, and glue the adductor muscle and the connection Separate the tissues at the top and carefully remove all the shellfish;

[0031] (B) adding distilled water and 0.1-0.3 mol / L hydrochloric acid solution ...

Embodiment 1

[0042] Example 1: Take PSP-positive scallops with a PSP content of 46.2 MU / g, wash the shell surface thoroughly with clean water, cut off the adductor muscle, open the shell, and rinse the interior with distilled water to remove impurities such as silt and other foreign substances , separate the adductor muscle from the tissue connected to the glued part, carefully remove all the shellfish, add distilled water, and 0.18mol / L hydrochloric acid solution to make the pH 3, homogenize, and place it in -80℃ for at least 4 hours after mixing. Hours, use a vacuum freeze dryer (CHRiST Epsilon 2-6D) to carry out primary freeze-drying to obtain a PSP positive sample, then carry out tissue grinding, pre-crushing, use a high-alumina porcelain ball mill to finely grind, pass through a 40-mesh sieve, and place it at -80 ° C for pre-treatment. Freeze for at least 4 hours, then freeze-dry again to remove moisture completely. In a room with constant temperature and humidity and aseptic operatio...

Embodiment 6

[0047] Embodiment 6 Uniformity and Stability Inspection

[0048] 6.1 Homogeneity test of samples

[0049] 16 bottles of samples prepared in Example 1 were randomly selected, and 3 groups of samples were taken for 3 times of repeatability detection in each bottle of samples. Use the mouse biological method test in GB / T 5009.213-2008 to analyze the PSP content of the standard sample, expressed in mouse units. All samples are tested in random order under repetitive conditions, that is, in the same laboratory by the same personnel using the same test methods and instruments for a short period of time.

[0050] The uniformity of the test results was evaluated by the single-factor multi-level analysis of variance method, and the following statistical formula was used for the single-factor multi-level analysis of variance. The results are shown in Table 2 and Table 3.

[0051] In order to test the uniformity of the samples, i samples are taken, and each sample is tested j times und...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a paralytic shellfish poisoning (PSP) standard sample and a preparation method and an application thereof. Positive shellfish raw materials of PSP are initially frozen and dried after being acidized and homogenized to obtain a crude sample, and then the crude sample is frozen and dried once again after being ground and sieved to obtain the product in the invention with good uniformity and stability. The standard sample can be used for capability assurance activities of PSP test items in labs, for PSP qualitative and quantitative detection and for verification of a detection method, correction of a tester and quality control and examination of test results. The invention has low raw material cost and simple preparation method, and the obtained standard sample is a material standard sample, is even and stable and easy for storage, and is beneficial for effective monitoring on shellfish poisoning.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a shellfish toxin standard sample and its preparation method and application, in particular to a paralytic shellfish toxin standard sample and its preparation method and application. Background technique [0002] In recent years, shellfish toxins have become an important public health problem. Many countries have controlled the limits of shellfish toxins in food. WHO has also issued a draft on the prevention of shellfish toxins and the protection of public health. my country is a major producer and exporter of shellfish in the marine economy, with an annual output of about 11 million tons of shellfish, accounting for 70% of the world's total. In the past 10 years, due to industrial pollution and other reasons, red tides have occurred frequently in my country's coastal waters, which has seriously affected the development of marine aquaculture and export trade. Since the incident of she...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N33/48G06Q90/00
Inventor 王秋艳曹际娟赵昕郑江
Owner 王秋艳
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com