54results about How to "Large amount of binding" patented technology

A melt processable, flexible copolymer of tetrafluoroethylene and fluorinated vinyl ether produced by suspension polymerization comprising: tetrafluoroethylene and fluorinated vinyl ether in an aqueous medium, a free radical initiator and a telomer The copolymerization is carried out in a pressurized, stirred reaction vessel, the aqueous medium is substantially free of fluorine-containing organic solvents. The contents of the reaction vessel were sufficiently agitated during the polymerization reaction to coagulate the copolymer of tetrafluoroethylene and fluorinated vinyl ether. Reduce the melt viscosity to less than about 1 x 10 6 The melt-processable, ductile copolymer in Pa.s was isolated directly from the reaction vessel.

The invention relates to the manufacture method for mouse nerve growth factor. The feature is that ultra-filtrating the supernatant of homogenated and centrifuged mouse submaxillary gland into pH value 6.8, 20mmol / L phosphate buffer solution through hollow fiber column. The Sepharose FF column would take chromatography to collect target albumen, and the Superdex 75prepgrade column collecting the target albumen to gain the mouse nerve growth factor raw liquor. The invention shortens the producing period and improves production efficiency.

The invention relates to a dual-clutch transmission starting control method which includes the steps of a, judging starting conditions; b, selecting a starting control mode; c, enabling power to be output through a first output shaft of a transmission after the transmission enters a first-gear starting control mode; d, enabling the power to be output through a second output shaft of the transmission after the transmission enters a second-gear starting control mode, and meanwhile conducting fine grinding smoothening processing with a second clutch control valve; e, enabling a TCU to judge whether a second-gear-to-first-gear control strategy is executed or not according to information transmitted by a clutch oil temperature sensor; f, enabling the TCU to judge whether a starting clutch fine smoothening grinding control mode is stopped or not according to gear shifting state information; g, enabling the TCU to judge whether the steps e-g are repeatedly executed or the steps f-g are repeatedly executed according to information transmitted by an engine rotation speed sensor and a transmission input shaft rotation speed sensor. According to the dual-clutch transmission starting control method, the defect that an exiting dual-clutch transmission is large in starting mode power loss and difficult to start under the low temperature working condition is overcome, and the comfort performance and the reliability of vehicle starting are improved.

The invention belongs to the field of molecular biology and mainly relates to a simple, efficient and universal type total RNA extraction kit and method. The kit is applicable to extraction of high-purity, high-integrity and high-yield total RNA from a majority of biological samples such as plant samples, animal samples, microorganism samples, blood samples as well as different tissue parts including roots, stems, leaves, flowers and fruits of plants, has the advantages of rapidness, convenience, efficiency, universality and the like, and pigments, grease and other impurities in the RNA extraction process can be simply and conveniently removed to the maximum extent due to a thin adsorption column. The extracted total RNA can be directly used for inverse transcription and other downstream molecular biology experiment operations.