54results about How to "Large amount of binding" patented technology

The invention discloses a method for modifying superfine fiber synthetic leather based on an amino-terminated hyperbranched polymer. The amino-terminated hyperbranched polymer is introduced into superfine fiber synthetic leather base cloth subjected to moderate acid hydrolysis through a cross-linking agent capable of crosslinking with amino and carboxyl to increase amino active groups, and then the cross-linking agent is added again to crosslink with gelatin hydrolysate to improve binding capacity of the gelatin hydrolysate and remarkably increase hydrophilic groups inside the base cloth. Simultaneously, a conventional natural leather processing technology is adopted to perform dyeing, grease adding, coating, middling and other operations to modified base cloth, and an imitated natural leather product with performance nearly equal to the natural leather performance is produced. By means of the method, hygienic performance and dyeing performance of products are nearly equal to those of the natural leather, and the products have elasticity, fullness and natural appearance of natural leather products. Simultaneously, the method does not relate to organic solvents, thereby solving the problem that a large number of organic solvents are used in the finishing process after existing superfine fiber synthetic leather is synthetized.

Disclosed is an oral administered compound colloid pectin bismuth preparation which comprises colloid pectine bismuth, antibiotics and antibacterial agent. The preparation process comprises dressing antibiotics and antibacterial agent micro-pellet core, mixing with colloid pectine bismuth and medically acceptable amount of excipient. After administration, the colloid pectine bismuth can form a membrane for the protection of gastric mucosa in stomach.

Disclosed is an oral administered compound colloid pectin bismuth preparation which comprises colloid pectine bismuth, antibiotics and antibacterial agent. The preparation process comprises dressing antibiotics and antibacterial agent micro-pellet core, mixing with colloid pectine bismuth and medically acceptable amount of excipient. After administration, the colloid pectine bismuth can form a membrane for the protection of gastric mucosa in stomach.

The invention discloses an affinity chromatographic packing for separating and purifying monoclonal antibody and antibodyglobulin and a preparation method thereof. The affinity chromatographic packing consists of a monodisperse porous microsphere solid phase carrier and a protein A ligand. The preparation method comprises: bonding the protein A onto the surface of the solid phase carrier through amino acids containing a nitrogen group; and thus obtaining the affinity chromatographic packing. Concretely, the method comprises three steps, namely connecting an activated spatial arm, coupling the ligand and sealing residual active groups. Meanwhile the invention also discloses an affinity chromatographic column made of the affinity chromatographic packing. The affinity chromatographic packing and the affinity chromatographic column made of the affinity chromatographic packing can be used in the production process of monoclonal antibody medicines and the separation and purification of antibodyglobulin (IgG), and have high separation and purification efficiency.

The invention discloses a processing method of a cellulosic fiber product with a photothermal effect, and belongs to the technical field of functional textile processing. The method comprises the following steps: firstly, introducing vinyl on a cellulose fiber product, and then catalyzing graft polymerization of acrylic acid and N,N-methylene bisacrylamide on a fiber by virtue of horse radish peroxidase, so as to form a net-shaped template containing carboxyl on the surface of the fiber; and then soaking the cellulose fiber product in a copper ammonia solution, and adding sodium sulfide to promote in-situ deposition on the surface of the fiber to form nano copper sulfide particles, so as to obtain the cellulose fiber product with the photothermal effect. According to the a processing method, the illumination self-heating cellulose fiber product is prepared by an adhesive-free method, and meanwhile, an illumination rapid sterilization function is endowed to the cellulose fiber product; and therefore, the cellulose fiber obtains the relatively high photothermal effect, and the application performance of the cellulose fiber product is improved.

The invention discloses a method for the large-scale production of neurotoxin in cobra venin and the reduction of the neurotoxicity, which comprises the following steps of: dissolving dry powder of cobra venin into Tris buffer solution of which the pH value is 7.5 and of which the concentration is 20 mmol / L, performing centrifugation and passing supernate through a 0.22 micrometer microfiltration membrane, putting samples on a CM Sepharose FF chromatographic column which is balanced with the solution; performing elution respectively by using balanced buffer solution containing sodium chloride to collect target albumen; removing the buffer solution by using a membrane bag of a 3K ultrafiltration membrane through ultrafiltration; adding a proper amount of hydrogen peroxide into desalinized albumen solution in proportion, after standing the mixture, degrading the hydrogen peroxide in the presence of a manganese dioxide catalyst, and performing filtration by using the 0.22 micrometer microfiltration membrane to remove the manganese dioxide so as to prepare finished products to be freeze-dried. Compared with the prior art, the method avoids adopting a purification step, improves the treatment capacity and yield, and is suitable for the large-scale production, and the neurotoxicity of the treated neurotoxin in the cobra venin is reduced greatly under the condition of invariable analgesic effect.

The invention provides a preparation method of high-performance carbon nanotube modified chrome tanning agent, which comprises the following steps of: after mixing a carbon nanotube with an acid solution, and ultrasonically processing the mixture to prepare a suspension; then, heating, condensing, refluxing, repeatedly washing and centrifugally separating until the pH value becomes neutral; subsequently, after dissolving dichromate in water, slowly adding sulfuric acid and the treated carbon nanotube; ultrasonically processing to prepare a stable suspension; taking glucose and adding water to dissolve the glucose; dropwise adding the water solution of glucose into the suspension containing dichromate while stirring so as to produce a carbon nanotube modified chrome tanning liquid in-situ; and finally, settling and placing for long time. The chrome tanning agent enhances the bonding force of chrome, increases the quality and the properties of leather, is friendly to environment, reduces pollution and decreases resource waste.

The invention discloses a magnetic particles chemiluminescence detection method. The method combines with the magnetic separation technology, the chemiluminescence technology, and the immunoassay technology. The method fully utilizes the rapid and easy automation performance of the magnetic separation technology, the high sensitivity performance of the chemiluminescence technology, and the specificity of the immunoassay, and exhibits an irreplaceable role in the field of biological analysis. Compared with a previous membrane strip immunoassay method and enzyme-linked immunosorbent assay method, the method has greatly improved the detection sensitivity, the detection range, the detection time, and the automation operation. According to the magnetic particles chemiluminescence detection method, the antigen-antibody binding surface area is large, and the detection sensitivity is improved by increasing the binding amount of the antigen-antibody; the reaction speed is fast, and the antigen-antibody can be rapidly captured; the combined phase and free phase are easy to separate, which can improve the detection accuracy; and the alkaline phosphatase luminescence system has good stability.

A melt processable, flexible copolymer of tetrafluoroethylene and fluorinated vinyl ether produced by suspension polymerization comprising: tetrafluoroethylene and fluorinated vinyl ether in an aqueous medium, a free radical initiator and a telomer The copolymerization is carried out in a pressurized, stirred reaction vessel, the aqueous medium is substantially free of fluorine-containing organic solvents. The contents of the reaction vessel were sufficiently agitated during the polymerization reaction to coagulate the copolymer of tetrafluoroethylene and fluorinated vinyl ether. Reduce the melt viscosity to less than about 1 x 10 6 The melt-processable, ductile copolymer in Pa.s was isolated directly from the reaction vessel.

The invention discloses a commercial and financial dual-purpose bundling machine. The commercial and financial dual-purpose bundling machine comprises a shell, wherein a belt storage box, a belt conveying mechanism and a cutting belt heat sealing mechanism are arranged in the shell. The commercial and financial dual-purpose bundling machine is characterized in that the shell is connected with a belt storage rack, a belt groove is formed in the inner side of the belt storage rack, a discharging space is formed in the middle of the belt storage rack, a bundling belt in the belt storage box can be driven by the belt conveying mechanism to be filled into the belt groove of the belt storage rack, and a movable baffle belt plate is arranged in the belt groove. According to the technical scheme,the bundling belt can be stably filled in the belt groove in the belt storage rack without dropping, and towel, scarf, paper currency, and bills can be convenient to bundle. The commercial and financial dual-purpose bundling machine can be used for both commerce and financial industry, the bundling amount is large, and the bundling effect is good.

The invention provides a Sundan red I immunoaffinity column which comprises a carrier, protein G and a Sundan red I antibody, wherein the protein G is coupled with the carrier through chemical bonds; the Sundan red I antibody is in cross-link with the protein G by using a cross-linking agent after being joined; the protein G is recombinant protein G which is based on GenBank CAA27638.1 and is subjected to gene recombinant expression. Under the condition that detection properties are not affected, the use amount of the Sundan red I antibody of a sepharose gel carrier pretreated with the protein G is about 3 / 4 of that of an antibody for preparing purification column packing by using a direct coupling method. The Sundan red I provided by the invention is very high in purity, can be directly used in high performance liquid chromatography detection without need of extra subsequent purification treatment, therefore, the time and the cost of operators can be saved.

The invention relates to a preparation method of a wave-transparent ablation and heat-insulation integrated material. The preparation method comprises the following steps: connecting a fabric high-density surface layer and a fabric low-density inner layer to form a whole body, so as to prepare a pre-formed body; preparing a hydrophobic coating on the fabric low-density inner layer of the pre-formed body to inhibit the wetting permeability of a water-based precursor; then circularly impregnating silica sol through a liquid-phase infiltration moulding technology; increasing the binding amount ofthe pre-formed body and the silica sol to improve the density of an outer surface through the circular impregnating of the silica sol; after reaching a certain density, removing a hydrophobic effectof the hydrophobic coating to obtain an integrated composite material blank; performing aerogel preparation on the integrated composite material blank without the hydrophobic effect of the hydrophobiccoating to obtain the wave-transparent ablation and heat-insulation integrated material. According to the preparation method provided by the invention, the integrated wave-transparent ablation and heat-insulation material is prepared through an integrated moulding preparation technology of a gradient density composite material which is initiated in the field; wave-transparent ablation and heat-insulation integration is realized through integrated moulding and the technical blank of the field is eliminated.

The invention discloses water-soluble oxidized chitosan and a finishing method of the water-soluble oxidized chitosan on a fabric. The water-soluble oxidized chitosan is prepared by the following steps: dissolving chitosan in a 1-carboxymethyl-3-methylimidazolium hydrogen sulfate aqueous solution, oxidizing C2 and C3 sites of the chitosan by sodium periodate under microwave radiation to generate aldehyde groups, and then oxidizing primary hydroxyl at C6 site of the dialdehyde chitosan in an ionic liquid by concentrated nitric acid-concentrated phosphoric acid to generate carboxyl. The water-soluble oxidized chitosan can be used for modifying fabrics. Compared with a traditional chemical cross-linking agent modified fabric, the method has the advantages that the grafting efficiency of the oxidized chitosan is high, the enzyme treatment condition is mild, the reaction process is simple, the fabric is crease-resistant, antibacterial, efficient and lasting, the fabric is comfortable to wear, and the requirements of people on lasting functional ecological textiles are met.

The invention discloses a flocculant for low-residue drinking water treatment and a preparation method thereof. The flocculant is formed by polymerizing sodium silicate, magnesium chloride or magnesium sulfate, aluminum sulfate or aluminum chloride and sodium aluminate, and a liquid product of the flocculant contains 7.3-9.2 weight percent of Al2O3, 0.54-.068 weight percent of SiO2 and 0.12-0.15 weight percent of MgO. The flocculant and the preparation method thereof have the beneficial effects that the flocculant is green and safe, the content of residual aluminum in flocculated effluent water is low, the residual aluminum quantity is remarkably lower than that of a traditional flocculant, and treated water achieves the relevant national urban water supply water quality aluminum content standard requirements. The flocculant has the advantages of small dosage, low effluent turbidity and stable product.

The invention belongs to the technical field of food safety inspection, and particularly relates to a tetracycline antibiotics immunoaffinity column and a preparation method and application thereof. Sepharose gel activated through an epichlorohydrin activation method is taken as a carrier, gene recombinant protein G is coupled to the carrier to obtain protein G-sepharose, a tetracycline antibiotics antibody is connected to the protein G-sepharose to obtain antibody-protein G-sepharose, a cross-linking agent is used for crosslinking, antibody-protein G-sepharose fillers are obtained, and finally, the immunoaffinity column is filled with the antibody-protein G-sepharose fillers. The specific binding feature of the gene recombinant protein G and tetracycline antibiotics is fully utilized, Fabfragments of the tetracycline antibiotics are exposed to the outside, and antibody fixation capacity of the gene recombinant protein G, capture capacity of the tetracycline antibiotics and purification efficiency of the purification efficiency are greatly improved.

The invention belongs to the field of molecular biology and mainly relates to a universal kit and method for extracting DNA from trace plant materials. The yield of DNA extracted once through the kit and the method can completely meet the requirement of direct whole genome sequencing. The kit and the method mainly comprise a thin silicon matrix adsorption column capable of adsorbing genome DNA to the maximum extent and a unique extraction buffering liquid system which is matched with the silicone matrix adsorption column and can extract various plant tissues and DNA in different parts of plants to the maximum extent. The kit and the method have the advantages of rapidness, convenience, no need of phenol-chloroform extraction, avoidance of organic solvent pollution and the like, and pigments, grease and other impurities in the DNA extraction process can be simply and conveniently removed to the maximum extent due to the thin adsorption column. Valuable plant samples are saved, and pretreatment steps and time of whole genome sequencing are greatly reduced and shortened.

The invention relates to the manufacture method for mouse nerve growth factor. The feature is that ultra-filtrating the supernatant of homogenated and centrifuged mouse submaxillary gland into pH value 6.8, 20mmol / L phosphate buffer solution through hollow fiber column. The Sepharose FF column would take chromatography to collect target albumen, and the Superdex 75prepgrade column collecting the target albumen to gain the mouse nerve growth factor raw liquor. The invention shortens the producing period and improves production efficiency.

The invention discloses a preparation method and applications of a Ni-NTA modified silicon dioxide coated ferroferric oxide magnetic nano functional assembly, and belongs to the field of materials. The preparation method comprises the following steps: preparing Fe3O4 nanoclusters by a solvothermal method; preparing Fe3O4@SiO2 magnetic nanoparticles by a St-ber sol-gel method; adding a silane coupling agent, and carrying out surface carboxylation of Fe3O4@SiO2 (Fe3O4@SiO2@COOH) through a chemical method; introducing an activating agent, and completing NTA modification (Fe3O4@SiO2@COOH@NTA) on the surfaces of the carboxylated Fe3O4@SiO2 magnetic nanoparticles; and adding Ni<2+> to prepare the assembly. According to the invention, the steps are clear, and the prepared magnetic nano functionalassembly is rich in surface groups, large in Ni<2+> binding capacity, high in binding force with His tag protein and high in separation efficiency.

The invention belongs to the technical field of molecular biology, and particularly discloses a reagent and a kit for rapidly extracting nucleic acid from an FFPE sample and application of the reagent and the kit. The combination buffer solution in the reagent is simple in formula and low in cost, the combination effect of the nucleic acid and the magnetic beads can be remarkably improved, and the combination amount of the nucleic acid is increased. According to the method, the lysis buffer solution, the binding buffer solution and the washing buffer solution with specific components are combined for use, tissue cells are fully lysed, protein bound with nucleic acid is digested, the nucleic acid is completely released, then the released nucleic acid is specifically bound to the magnetic beads, the binding amount of the nucleic acid is increased, effective separation of the nucleic acid and other lysates is achieved, the magnetic beads combined with the nucleic acid are washed for multiple times, impurities such as protein and lipid are thoroughly removed, and the nucleic acid isfinally eluted from the magnetic beads to obtain high-purity and high-yield nucleic acid. The reagent / kit is simple in composition, low in cost and convenient for nucleic acid extraction operation, and the extraction time can be greatly shortened.

The invention discloses a preparation method of micron-sized biological magnetic beads for purifying free DNA. The method is simple in process, and the prepared magnetic beads are of a core-shell structure, uniform in particle size, monodisperse, free of agglomeration, high in magnetic response performance, rough in surface, rich in morphology, large in specific surface area and rich in surface hydroxyl content. The magnetic beads can be used for purifying free DNA samples.

The invention relates to a dual-clutch transmission starting control method which includes the steps of a, judging starting conditions; b, selecting a starting control mode; c, enabling power to be output through a first output shaft of a transmission after the transmission enters a first-gear starting control mode; d, enabling the power to be output through a second output shaft of the transmission after the transmission enters a second-gear starting control mode, and meanwhile conducting fine grinding smoothening processing with a second clutch control valve; e, enabling a TCU to judge whether a second-gear-to-first-gear control strategy is executed or not according to information transmitted by a clutch oil temperature sensor; f, enabling the TCU to judge whether a starting clutch fine smoothening grinding control mode is stopped or not according to gear shifting state information; g, enabling the TCU to judge whether the steps e-g are repeatedly executed or the steps f-g are repeatedly executed according to information transmitted by an engine rotation speed sensor and a transmission input shaft rotation speed sensor. According to the dual-clutch transmission starting control method, the defect that an exiting dual-clutch transmission is large in starting mode power loss and difficult to start under the low temperature working condition is overcome, and the comfort performance and the reliability of vehicle starting are improved.

The invention relates to a dual-clutch transmission starting control method which includes the steps of a, judging starting conditions; b, selecting a starting control mode; c, enabling power to be output through a first output shaft of a transmission after the transmission enters a first-gear starting control mode; d, enabling the power to be output through a second output shaft of the transmission after the transmission enters a second-gear starting control mode, and meanwhile conducting fine grinding smoothening processing with a second clutch control valve; e, enabling a TCU to judge whether a second-gear-to-first-gear control strategy is executed or not according to information transmitted by a clutch oil temperature sensor; f, enabling the TCU to judge whether a starting clutch fine smoothening grinding control mode is stopped or not according to gear shifting state information; g, enabling the TCU to judge whether the steps e-g are repeatedly executed or the steps f-g are repeatedly executed according to information transmitted by an engine rotation speed sensor and a transmission input shaft rotation speed sensor. According to the dual-clutch transmission starting control method, the defect that an exiting dual-clutch transmission is large in starting mode power loss and difficult to start under the low temperature working condition is overcome, and the comfort performance and the reliability of vehicle starting are improved.

The invention belongs to the field of molecular biology and mainly relates to a simple, efficient and universal type total RNA extraction kit and method. The kit is applicable to extraction of high-purity, high-integrity and high-yield total RNA from a majority of biological samples such as plant samples, animal samples, microorganism samples, blood samples as well as different tissue parts including roots, stems, leaves, flowers and fruits of plants, has the advantages of rapidness, convenience, efficiency, universality and the like, and pigments, grease and other impurities in the RNA extraction process can be simply and conveniently removed to the maximum extent due to a thin adsorption column. The extracted total RNA can be directly used for inverse transcription and other downstream molecular biology experiment operations.

The invention belongs to the field of aquaculture, and discloses an artificial breeding method for homatula variegata. The method comprises the following steps of parent fish selection: selecting parent fish in sexual maturity and age of 3 years or above; parent fish breeding: feeding spawning induction feed in one week before spawning induction, wherein 5-12 grams of biological oxytocin is addedinto the spawning induction feed per kilogram, and feeding is continuously conducted for one week; artificially induced spawning and fertilization: in the breeding season of the homatula variegata, selecting parent fish in sexual maturity, and injecting oxytocin to induce spawning and spermiation; evenly spreading fertilized ova in an incubator for incubation; fry breeding: after the fertilized ova in the incubator are all hatched for 2-7 days, feeding cooked egg yolks and fish meat; after 7-10 days of incubation, conducting transferring into a fry breeding pond, feeding initial feed, gradually using mixed feed for food domestication when the fry body length reaches 2 cm, and cleaning and disinfecting the pond to prevent diseases. The artificial breeding method greatly shortens the time ofbreeding of the homatula variegata, improves the fertilization rate and hatching rate, and improves the yield of the homatula variegata.

The invention relates to the field of cake additives, and particularly discloses a cake emulsifier with dense and fine texture and a preparation method thereof. The cake emulsifier with dense and fine texture is characterized by being prepared from the following raw materials in parts by weight: 10-25 parts of glyceryl monostearate; 2 to 10 parts of polyglycerol fatty acid ester; 2 to 10 parts of sorbitan monostearate; 0.5 to 3 parts of potassium stearate; 0.5 to 3 parts of propylene glycol monostearate; 2 to 15 parts of glycerol; 2 to 15 parts of sorbitol; 10 to 25 parts of propylene glycol; and 35-55 parts of water. According to the cake emulsifier with dense and fine texture, the stability of an egg paste foam system can be enhanced, the obtained cake can keep a good dense and loose structure, and then the baked cake has a soft texture structure and is excellent taste.

The invention discloses a sensitization test strip for rapidly detecting cronobacter sakazakii and application of the sensitization test strip, and belongs to the technical field of detection of cronobacter sakazakii. The sensitization test strip comprises a sample pad, a gold standard pad, an NC film, an absorbent pad and a bottom plate, wherein the NC film is provided with a detection line and acontrol line, the control line is coated with a goat anti-mouse secondary antibody, and a detection line solution coating the detection line is obtained by incubating colloidal gold and an anti-cronobacter sakazakii capture antibody. The sensitization test strip has the characteristic of being capable of improving the protein binding quantity on the NC film, thereby enhancing the capture rate; the colloidal gold added at the detection line can improve aggregation coloration, thereby improving the detection effect; and the sensitivity is improved by 100 times compared with a traditional test strip, and the sensitization test strip has high specificity, short detection time, easy manufacture and a low cost, and is easy to carry and suitable for on-site detection of cronobacter sakazakii.

The invention discloses an air tightness detection device for a shell die casting, and the device comprises a bottom plate which is provided with at least one die casting positioning region. The lifting pressing plate is arranged above the bottom plate to ascend and descend, and a plurality of die casting pressing assemblies are vertically installed at the lower end of the lifting pressing plate; the sealing groove encloses the die casting positioning area, and a sealing strip is arranged in the sealing groove in an embedded mode; the arc-shaped grooves are formed in the two ends of the die casting positioning area, and the ends of the sealing grooves extend to be communicated with the arc-shaped grooves; the end sealing columns are installed at the two ends of the die casting positioning area and embedded into the arc-shaped grooves, and the outer sides of the end sealing columns are connected with sealing rings in a sleeved mode, and the sealing rings make contact with the ends of the sealing strips. And the extrusion sleeve is axially arranged on one side of the end sealing column and is driven by an execution component on the bottom plate to axially move to extrude the sealing ring. According to the invention, the material cost of the sealing strip is not increased under the condition that the product structure is not allowed to be changed.

Provided is a method for artificial propagation of giant salamanders. The method includes the following steps of: (1) selection of fish species; (2) artificial inducement for spawning; (3) artificialegg supporting and fertilization: after semen is squeezed, the squeezed semen is diluted with 3-5 ml of diluent, the diluted semen is mixed with chalaza, sperm-egg binding is achieved through gentle shaking, a small amount of clear water is added after 5-10 minutes, water is changed twice after 30 minutes, and incubation is performed after the obtained mixture is divided into different basins; (4)artificial incubation and management; (5) juvenile fish feeding; and (6) growth of the giant salamanders. The method has the advantages of short fertilization time, a large amount of bound sperms andeggs, a high hatching rate, short propagation time and a high survival rate.

The invention belongs to the field of molecular biology and mainly relates to a simple, efficient and universal type total RNA extraction kit and method. The kit is applicable to extraction of high-purity, high-integrity and high-yield total RNA from a majority of biological samples such as plant samples, animal samples, microorganism samples, blood samples as well as different tissue parts including roots, stems, leaves, flowers and fruits of plants, has the advantages of rapidness, convenience, efficiency, universality and the like, and pigments, grease and other impurities in the RNA extraction process can be simply and conveniently removed to the maximum extent due to a thin adsorption column. The extracted total RNA can be directly used for inverse transcription and other downstream molecular biology experiment operations.