The invention discloses a sensitization test strip for rapidly detecting cronobacter sakazakii and application of the sensitization test strip, and belongs to the technical field of detection of cronobacter sakazakii. The sensitization test strip comprises a sample pad, a gold standard pad, an NC film, an absorbent pad and a bottom plate, wherein the NC film is provided with a detection line and acontrol line, the control line is coated with a goat anti-mouse secondary antibody, and a detection line solution coating the detection line is obtained by incubating colloidal gold and an anti-cronobacter sakazakii capture antibody. The sensitization test strip has the characteristic of being capable of improving the protein binding quantity on the NC film, thereby enhancing the capture rate; the colloidal gold added at the detection line can improve aggregation coloration, thereby improving the detection effect; and the sensitivity is improved by 100 times compared with a traditional test strip, and the sensitization test strip has high specificity, short detection time, easy manufacture and a low cost, and is easy to carry and suitable for on-site detection of cronobacter sakazakii.