Method for large-scale production of neurotoxin in cobra venin and reduction of neurotoxicity

Abstract

The invention discloses a method for the large-scale production of neurotoxin in cobra venin and the reduction of the neurotoxicity, which comprises the following steps of: dissolving dry powder of cobra venin into Tris buffer solution of which the pH value is 7.5 and of which the concentration is 20 mmol / L, performing centrifugation and passing supernate through a 0.22 micrometer microfiltration membrane, putting samples on a CM Sepharose FF chromatographic column which is balanced with the solution; performing elution respectively by using balanced buffer solution containing sodium chloride to collect target albumen; removing the buffer solution by using a membrane bag of a 3K ultrafiltration membrane through ultrafiltration; adding a proper amount of hydrogen peroxide into desalinized albumen solution in proportion, after standing the mixture, degrading the hydrogen peroxide in the presence of a manganese dioxide catalyst, and performing filtration by using the 0.22 micrometer microfiltration membrane to remove the manganese dioxide so as to prepare finished products to be freeze-dried. Compared with the prior art, the method avoids adopting a purification step, improves the treatment capacity and yield, and is suitable for the large-scale production, and the neurotoxicity of the treated neurotoxin in the cobra venin is reduced greatly under the condition of invariable analgesic effect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine extraction, and in particular relates to a method for extracting neurotoxin from cobra snake venom and modifying it to reduce neurotoxicity. Background technique [0002] Cobra venom is a natural poisonous protein secreted by the venom glands of cobras. Its chemical composition is very complex, containing a variety of proteins, polypeptides, enzymes and other small molecules, and has a wide range of biological activities. With the development of modern biotechnology, many components of cobra venom have been isolated, purified and sequenced, and various components are widely used in theoretical research and clinical application of biochemistry, molecular biology, toxicology and pharmacology. [0003] Since Monaelesser and Taguet first reported the significant curative effect of cobra venom on the cases of pain caused by cancerous tissue compression of nerves in 1933, the analgesic effect of cob...

AI Technical Summary

Problems solved by technology

[0004] The original preparation method of cobra snake venom neurotoxin usually adopts two-step purification, using ion exchange resin and molecular sieve for rough purification and fine purification respectively, which has many steps and long production cycle, which leads to low recovery rate

And although the cobra snake venom neurotoxin has a good analgesic effect, it also has considerable neurotoxicity, which will cause safety hazards during use