The invention discloses an alkali-proof chopped glass fiber impregnating compound, and relates to a preparation method of a glass fiber surface treating agent containing an organic material. The preparation method comprises the following steps: mixing deionized water and glacial acetic acid and evenly stirring; adding a silane coupling agent, a polyacrylate film-forming agent, an epoxy resin emulsion film-forming agent, a water-soluble epoxy film-forming agent, an organosilicone lubricant and a cationic lubricant which are diluted by deionized water, and an antistatic agent diluted by deionized water; and finally adding deionized water and stirring for 15-25 minutes, wherein the stirring speed is 60r/m, wherein the film-forming agents are the polyacrylate film-forming agent, the epoxy resin emulsion film-forming agent and the water-soluble epoxy film-forming agent, the mass ratio of the polyacrylate film-forming agent to the epoxy resin emulsion film-forming agent to the water-soluble epoxy film-forming agent is (2.0-20.0) to (15.0-2.5) to (1.0-10). The alkali-proof chopped glass fiber impregnating compound disclosed by the invention is suitable for an alkali-proof chopped glass fiber of 4-24mm; the impregnated alkali-proof chopped glass fiber has good alkali resistance, bundling property, abrasive resistance, water tolerance, choppability, dispersibility and antistatic property, and cannot disperse in a cement mixing and agitating process; the bundling state of a precursor fiber is kept.