4results about How to "Promote in-depth" patented technology

The invention is applicable to the technical field of biotechnology, and particularly relates to a primer composition for amplifying a coding sequence of a human B cell receptor heavy chain CDR3 and application of the primer composition, and two primer groups, namely a first primer group and a second primer group, are designed. The two primers are respectively used as an upstream primer and a downstream primer, and comprise at least one human IGH CDR3 upstream primer and at least one human IGH CDR3 downstream primer. The primers can be respectively combined with nucleic acid of human B cells, and a basis for researching a CDR3 region of a B cell receptor is provided. The first primer group and the second primer group are combined with the nucleic acid of the human B cell, so that the coding sequence of the heavy chain of the human B cell receptor is effectively enriched, part of pseudogenes are contained, the amplification efficiency of the heavy chain CDR3 library of the human B cell receptor can be improved, and the deep research on immune response of the human B cell is promoted.

The invention discloses a shallow buried aerated drip irrigation cultivation method for silage corn in slightly salinized farmland, and relates to the field of agricultural irrigation. The cultivation method of the present application aims at the problems of easy compaction of salinized farmland soil, low soil fertility, and low crop biomass in the Hetao irrigation area, and combines two irrigation technologies, shallow buried drip irrigation and aerated drip irrigation, to achieve high yield and high quality of silage corn in saline-alkali land. Shallow buried drip irrigation promotes water exchange between the surface layer and the middle and deep layers of the soil, ensuring water supply during the growth period, and aerated drip irrigation achieves effective oxygenation of irrigation water, improves soil alkaline phosphatase, urease, and catalase activities to promote soil urea decomposition, oxidation-reduction, and aeration improvement, which can significantly improve the biomass and quality of silage corn, improve farmers' planting intentions, enhance environmental friendliness, and greatly improve the mechanization level of silage corn production, easy operation, and reduce manual labor.

This invention discloses a combined process for restoring reservoir drainage and gas production channels using high-pressure drive and chemical dissolution, relating to the field of efficient oilfield exploitation technology. The process includes: S1, conducting production profile testing and sampling analysis of blockages in the target gas well to determine the type, distribution location, and reservoir physical parameters of the blockages, and preparing a corresponding chemical dissolving agent based on the analysis results. This invention couples the mechanical fracture-creating effect of high-pressure pulsed fluid with the cavitation effect and vibrational mass transfer effect of ultrasound. Based on the initial dissolution of blockages by the chemical dissolving agent, the stress waves generated by the high-pressure pulse expand the reservoir microfractures. Simultaneously, the collapse of microbubbles generated by ultrasound enhances the dissolution reaction rate, and the vibration promotes the dissolving agent's penetration into the micropores, achieving a synergistic effect of chemical dissolution and physical displacement, thoroughly removing blockages from the pore throats, and avoiding secondary precipitation of dissolution products.

The invention relates to the technical field of biomass charcoal loaded nanoscale zero-valent iron production equipment, in particular to a biomass charcoal loaded nanoscale zero-valent iron production device and a production method thereof.The biomass charcoal loaded nanoscale zero-valent iron production device comprises a fixed chassis, supporting pipes are installed on the outer side of the fixed chassis in the circumferential direction of the fixed chassis at equal angles, and positioning circular rings are placed at the tops of the supporting pipes; a cylinder is fixedly installed on the inner side of the positioning circular ring, the inner wall of the cylinder is a regularly-arranged concave-convex face, a discharging opening is formed in the position close to the bottom of the cylinder, a sealing ring is concentrically welded to the bottom of the cylinder, a disc is rotatably installed on the inner ring of the sealing ring, and a penetrating hole is formed in the center of the disc. Circular holes are symmetrically formed in the disc with the center line of the penetrating hole as the axis. Main supporting plates are horizontally mounted in the middle sections of the outer sides of the supporting pipes, and auxiliary supporting plates are welded to the outer sides of the two symmetrical main supporting plates in an extending manner; a three-dimensional turbulent flow field can be formed in the cylinder, and the cleaning effect on the biomass charcoal loaded nanoscale zero-valent iron is improved.