Extraction and purification process of shellfish toxins

Abstract

The present invention relates to a process for extracting and purifying shellfish toxins. First, the purification, identification and expanded cultivation of toxin-producing algal species are carried out. Natural seawater samples are continuously separated, purified and cultivated, and the species is determined morphologically by scanning electron microscopy. The full-length 18SrDNA sequence was obtained from the PCR product and sequenced, and the sequence comparison and analysis was carried out at NCBI through Blast to confirm its species, and then optimize the culture conditions of the target algae species, such as the composition of the culture medium, pH value, temperature, Light, salinity, culture time, etc., and use optimized conditions to expand the culture, and then extract and purify to obtain shellfish toxins. The process provided by the invention not only increases the content of the shellfish toxin in the culture solution and is beneficial to later extraction, but also has simple extraction and purification steps, high yield and low cost, and is beneficial to large-scale industrial application.

Description

technical field [0001] The invention relates to a process for extracting and purifying marine toxins, in particular to a process for extracting and purifying shellfish toxins. Background technique [0002] Marine toxins are toxic polymer compounds formed by the toxic algae in the ocean passing through the food chain to algae-eating fish, shrimp and shellfish, and accumulating in their bodies. [0003] There are many types of marine toxins, among which shellfish toxins are one of the most harmful. Shellfish poisons include paralytic shellfish poisons (PSP), diarrhea shellfish poisons (DSP), neuropathic shellfish poisons (NSP) and amnestic shellfish poisons (ASP). The hazards of shellfish toxins are sudden and extensive. Due to their high toxicity, quick response and no suitable antidote, many difficulties have been brought to the control. Therefore, the study of shellfish toxins is of great significance. [0004] At present, shellfish toxins are also one of the fastest-grow...

AI Technical Summary

Problems solved by technology

[0005] For the purification, identification and expansion of the important toxin-producing algal species of okadaic acid (OA), saxitoxin (STX), fintoxin (DTX) and scallop toxin (YTXs), natural seawater samples were passed through continuous Separation, purification, cultivation, using electron microscope scanning to determine the species morphologically, further obtaining the full-length 18SrDNA sequence through PCR products and sequencing, and performing sequence comparison analysis through Blast at NCBI to confirm its species and optimize the target algae species Culture conditions, such as culture medium, temperature, light, salinity, container, etc., and use optimized conditions to expand the culture, but the culture medium obtained by the current culture method has a low content of shellfish toxins, which is not conducive to later extraction. Moreover, the extraction and purification steps are cumbersome and the yield is low