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Standard sample of diarrhetic shellfish toxin and preparation method of standard sample

A shellfish toxin and standard sample technology, applied in the field of marine aquatic products detection, can solve the problems of high price and no matrix

Inactive Publication Date: 2012-10-03
徐君怡 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, DSP and PSP standard products are single-component standard pure products, without matrix, and the price is quite expensive. Each component is as high as about 6000 yuan / 100μg. There are strict regulations on the entry, exit and use of shellfish toxins

Method used

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  • Standard sample of diarrhetic shellfish toxin and preparation method of standard sample
  • Standard sample of diarrhetic shellfish toxin and preparation method of standard sample
  • Standard sample of diarrhetic shellfish toxin and preparation method of standard sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1 Raw material selection:

[0025] Diarrhetic shellfish poisons (DSP) were detected by mussels grown in the natural sea area of ​​Zhangzi Island, Dalian, by mouse biological method and enzyme-linked immunosorbent assay [1-3] content.

[0026] 2. Determination of diarrheal shellfish toxin in raw materials:

[0027] According to the determination method of SN / T 1996-2007, the DSP in the above-mentioned collected mussels was determined by the experiment. Wash the shell thoroughly with clean water, cut off the adductor muscle, open the shell, rinse the inside with heavy distilled water to remove sediment and other foreign matter. The adductor muscle is separated from the tissue attached to the ligament, and the midgut gland is carefully removed. Collect about 200g and place it in a sieve to drain for 5 minutes (do not allow the meat to accumulate), and detect debris such as broken shells.

[0028] Take 10 g of midgut gland, add 5 times the volume of 90% methanol solutio...

Embodiment 2

[0050] Example 2 Uniformity

[0051] In order to check the uniformity of the prepared standard samples of diarrheal shellfish poisons (DSP), 15 bottles of samples were randomly selected, and each bottle of samples was tested twice for repeatability. Use the enzyme-linked immunosorbent assay test in SN / T 1996-2007 to analyze the DSP content of the standard sample, expressed in μg / g. All samples are tested in random order under repetitive conditions, that is, in the same laboratory by the same personnel using the same test methods and instruments for a short period of time.

[0052] The data statistics formula is as follows:

[0053] In order to test the uniformity of the samples, i samples are taken, and each sample is tested j times under repeated conditions.

[0054] Test average for each sample

[0055] Overall average of all samples tested

[0056] Total number of tests

[0057] Between-sample sum of squares SS 1 = ...

Embodiment 3

[0068] Embodiment 3 Stability

[0069] The packaged samples selected for stability testing have been stored for a long time at low temperatures of -18~-20°C. According to the sampling principle of dense at the beginning and sparse at the time interval, the packaged samples for stability testing were randomly selected at 0, 2, 4, 6, 9, 12, 18 and 24 months respectively. Three samples were selected for testing each time. Use the enzyme-linked immunosorbent assay in SN / T 1996-2007 to analyze the diarrheal shellfish toxin (DSP) content of the standard sample, expressed in μg / g. In all stability tests, the personnel, instruments, test methods and laboratories used are the same as the uniformity tests. Keep away from light, seal, store at a temperature below -20°C, and be stable within two years. The test results are shown in Tables 3 and 4.

[0070] Table 3 Stability test results of diarrheal shellfish toxin

[0071]

[0072] Table 4 Stability evaluation results of standard...

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Abstract

The invention discloses a standard sample of diarrhetic shellfish toxin and a preparation method of the standard sample. Marine mussels are taken as materials, and research on a key preparation technology of a quality control sample of the diarrhetic shellfish toxin is emphasized to conduct from the aspects of sample impurity-removal and homogenization, freeze-drying, sample sub-packaging, determination and the like; on the basis, homogenization and stability testing is carried out; and the content reference value of the quality control sample of the diarrhetic shellfish toxin is determined by determination by a value fixing method and statistical treatment on fixed value data, and the standard sample of the diarrhetic shellfish toxin for biological detection is developed. The preparation of the standard sample has important practical significance and application value in comprehensively and deeply researching ingredient detection of diarrhetic shellfish toxin and the preparation technology and a stability ensuring technology of the standard sample, actively developing detection on the standard sample of the shellfish toxin, and filling up the blank in the detection field.

Description

technical field [0001] The invention belongs to the technical field of detection of marine aquatic products, and in particular relates to a standard sample of diarrhea shellfish toxin and a preparation method thereof. Background technique [0002] In recent years, shellfish toxins have become an important public health problem. Many countries have controlled the limits of shellfish toxins in food. WHO has also issued a draft on the prevention of shellfish toxins and the protection of public health. my country is a major producer and exporter of shellfish in the marine economy, with an annual output of about 11 million tons of shellfish, accounting for 70% of the world's total. In the past 10 years, due to industrial pollution and other reasons, red tides have occurred frequently in my country's coastal waters, which has seriously affected the development of marine aquaculture and export trade. Since the incident of shellfish poisoning from eating Chinese imports occurred in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 徐君怡王秋艳王刚
Owner 徐君怡
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