DSP (diarrhetic shellfish poison) detection and analysis method based on cell image sensor

An image sensor and sensor technology, applied in image analysis, image data processing, individual particle analysis, etc., can solve the problems of large equipment, high detection cost, high price, etc., and achieve the effect of low cost, simple operation steps and low cost

Inactive Publication Date: 2015-01-07
ZHEJIANG UNIV
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Problems solved by technology

Although HPLC-MS has the advantages of a wide range of detection, its equipment is bulky and expensive
ELISA an

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  • DSP (diarrhetic shellfish poison) detection and analysis method based on cell image sensor
  • DSP (diarrhetic shellfish poison) detection and analysis method based on cell image sensor
  • DSP (diarrhetic shellfish poison) detection and analysis method based on cell image sensor

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Embodiment Construction

[0033] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited.

[0034] The method for detecting and analyzing shellfish diarrheal toxins based on a cell image sensor in the present invention is implemented on a shellfish diarrheal toxin detection and analysis system, such as figure 1As shown, the shellfish diarrhea toxin detection and analysis system includes: CCD camera 1, cardiomyocyte sensor, inverted microscope 3 and computer 5; wherein, cell image sensor 2 is fixed on the stage of inverted microscope 3; CCD camera 1 is fixed on Above the inverted microscope 3, the image signal of the cell image sensor culture plate 1 is collected; the CCD camera 1 is connected with the computer 5 through the USB connection line 4, so that the collected signal is transmitted to the computer 5 for post-processing; the method includes the following steps:

[0035] (1) Culture of...

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Abstract

The invention discloses a DSP (diarrhetic shellfish poison) detection and analysis method based on a cell image sensor. The method is characterized by comprising the following steps: establishing the cell image sensor which is high in performance and low in cost, acquiring an image, converting an acquired RGB (red green blue) image to a grayscale image, binaryzating the image, matriculating the image, detecting a peak value, detecting the variation of a pulse image of a myocardial cell when in mechanical pulse, and detecting the mechanical pulse rate, pulse range and pulse interval of the myocardial cell; establishing a standard graph by detecting the response of the cell image sensor to different standard sample working solutions of the shellfish diarrhetic poisons in different concentrations; and detecting the response of the cell image sensor to the shellfish diarrhetic poisons of unknown concentration, comparing the detected response result with the standard graph, and semi-quantitatively calculating the concentration of the poisons. Compared with the existing shellfish diarrhetic poison detection and analysis method, the method is low in cost, simple in operation and capable of directly evaluating the toxicity of the poisons for a long time.

Description

technical field [0001] The invention relates to a shellfish diarrhea toxin detection and analysis technology, in particular to a shellfish diarrhea toxin detection and analysis method based on a cell image sensor. Background technique [0002] Diarrheal toxins in marine aquatic products are a kind of highly toxic non-protein toxins, which are formed by the accumulation of harmful organic substances in the body of shellfish by preying on planktonic algae. People can cause poisoning and even death after eating. At present, the detection methods of diarrheal toxins in marine aquatic products in China usually use mouse biological assay (MBA), high performance liquid chromatography-mass spectrometry (HPLC-MS) and enzyme-linked immunoassay technology (ELISA). The amount of experiments required by the MBA method is huge and there are great individual differences, and the virulence level of the toxin may be underestimated. Although HPLC-MS has the advantages of a wide detection ra...

Claims

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Application Information

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IPC IPC(8): G01N15/10G06T7/00
Inventor 王平苏凯麒邹玲王琴胡宁黎洪波邹瞿超曹端喜
Owner ZHEJIANG UNIV
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