Surface plasma resonance rapid detection method for paralytic shellfish poisoning

A technology of surface plasmon and detection method, which is applied in the field of rapid detection of ion plasma resonance, can solve the problems of high cost of ELISA technical analysis, reduced operability, etc., and achieve the effects of easy wide promotion, short detection time and improved sensitivity

Inactive Publication Date: 2005-04-06
NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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Problems solved by technology

However, the cost of ELISA technology analysis is relatively high, especially the reagents used need to be labeled, which reduces its operability and has not been widely used.

Method used

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Embodiment Construction

[0036] The invention is a surface plasmon resonance rapid detection method of paralytic shellfish poisoning. It uses a SPR detector with a surface plasmon resonance sensor to detect intermolecular interactions with the principle of near-field optics. The detection laser of the surface plasmon resonance sensor is incident between the prism and the gold film coated on the surface of the prism. On the interface of the chip, the intensity and phase of the reflected light change significantly. The intensity and number of receptors in the sample solution combined with the ligands on the chip are different, and the intensity and phase of the reflected light are also different. The change in this signal is closely related to the refractive index of the material next to the metal surface. If PSP molecules are immobilized on the metal surface, free PSP antibody molecules in the solution can be detected, thereby indirectly determining the PSP content in the sample to be tested. It is ch...

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Abstract

This invention discloses a paralysis shellfish poison surface plasm resonance rapid measurement, which belongs to environment chemistry and biology molecule detecting field. The method is the following: to add quantitative PSP anti-body to the PSP sample solvent; then to flow on the gold sputtered plate with PSP solidified; to measure the over PSP anti-body in the solvent by use of plasm resonance sensor SPR detector to indirectly measure the concentration of the PSP solvent. This method is especially applied in quantitative study and regular or emergency monitor of PSP in freshwater algae or sea red tide.

Description

technical field [0001] The invention belongs to the field of environmental chemistry and biomolecular detection, and particularly relates to a paralytic method for detecting the content of PSP in a solution by detecting the residual amount of paralytic shellfish poison (PSP) antibody in a sample solution by using surface plasmon resonance (SPR) technology. Surface plasmon resonance rapid detection method for shellfish poisoning. Background technique [0002] Red tide is a global marine environmental disaster. The occurrence of red tide poses a serious threat to the marine ecological environment, marine resources and public health, and the resource and economic losses it causes are even more difficult to estimate. Among the toxins produced by harmful red tides, the paralytic shellfish poison (PSP) toxin is the most widely distributed and the most harmful in the world. PSP has high specific toxicity to the biological nervous system and cardiovascular system, and is a highly s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/17G01N33/531G01N33/553
Inventor 王菊英罗昭锋鲍军波陈宇春
Owner NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
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