Capillary electrophoresis electrochemical enzyme linked immunoassay for detecting amnesic shellfish toxicity

A technology of capillary electrophoresis and shellfish toxins, which is applied in the direction of material analysis, material analysis, and measuring devices through electromagnetic means, can solve problems that have not been reported, and achieve the effects of less reagent consumption, high separation efficiency, and high sensitivity
CN101949931AInactive Publication Date: 2011-01-19QINGDAO UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
QINGDAO UNIV OF SCI & TECH
Publication Date
2011-01-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses capillary electrophoresis electrochemical enzyme linked immunoassay for detecting amnesic shellfish toxicity, which belongs to the technical field of assay and detection. Solution of standard substances and shellfish samples with the amnesic shellfish toxicity are assayed by combining the capillary electrophoresis technology and electrochemical enzyme linked immunoassay technology. The capillary electrophoresis electrochemical enzyme linked immunoassay comprises the following steps of: reacting sample solution and an amnesic shellfish toxicity antibody marked by a horse radish peroxidase (HRP) in a noncompetitive mode; separating an amnesic shellfish toxicity-enzyme labeled antibody compound and the rest amnesic shellfish toxicity antibody marked by the HRP in the mixed solution by capillary electrophoresis; generating a 3-aminophenazine with electrochemical activities by using aminophenol which is the oxidogenic substrate of peroxide in the presence of a catalyst; and performing electrochemical detection. The method simplifies a sample treatment process and has high selectivity and high accuracy. The linear detection range of the solution of amnesic shellfish toxicity standard substances of the method is 0.5 to 50ng / mL and a detection limit of the method is 0.2ng / mL. The method is an ideal method for detecting the amnesic shellfish toxicity of shellfish samples.
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Description

technical field

[0001] The invention relates to capillary electrophoresis electrochemical enzyme-linked immunoassay technology, in particular to capillary electrophoresis electrochemical enzyme-linked immunoassay method for detecting amnestic shellfish toxin. Background technique

[0002] The ever-increasing environmental pollution has led to an increase in the types and quantities of red tide toxins in the ocean. These red tide toxins are transmitted through the food chain and threaten human health. The inspection of red tide toxins in imported aquatic products by countries around the world is becoming more and more stringent, and it is urgent to develop a fast, sensitive and reliable method for detecting red tide toxins in the ocean.

[0003] Red tide toxin is also known as "shellfish toxin". According to the poisoning symptoms of the human body, it can be further divided into paralytic shellfish poisoning (PSP), diarrhea shellfish poisoning (DSP), amnestic shellfish pois...

Claims

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