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Method for detecting 13 shellfish toxins in biological sample

A technology for biological testing materials and shellfish toxins, applied in material separation, analysis materials, measuring devices, etc., can solve the problems of high detection limit, insufficient sensitivity, and few reports on shellfish toxin detection, so as to reduce the detection limit , good repeatability, improved sensitivity and repeatability

Pending Publication Date: 2021-11-05
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Claims
  • Application Information

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Problems solved by technology

At present, the detection of shellfish toxins is mostly aimed at the shellfish itself or related food raw materials, but there are few reports on the detection of shellfish toxins in biological samples
And to the detection of shellfish toxin in food raw material, its detection limit is often higher (generally ≥ 2ng / mL), for the detection of shellfish toxin in the biological test material, sensitivity is not enough; The detection method of shellfish toxins in food raw materials is directly applied to biological samples

Method used

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  • Method for detecting 13 shellfish toxins in biological sample
  • Method for detecting 13 shellfish toxins in biological sample
  • Method for detecting 13 shellfish toxins in biological sample

Examples

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Embodiment Construction

[0050] 1 Reagents and materials

[0051] 1.1 Reagents

[0052] The water used in this embodiment complies with the third-grade water specified in GB / T6682. Unless otherwise stated, the reagents used in the analysis were of analytical grade, including:

[0053] a) acetonitrile (chromatographically pure);

[0054] b) formic acid (chromatographically pure);

[0055] c) acetic acid (excellent pure);

[0056] d) ammonia water (excellent grade, concentration ≥ 25%);

[0057] e) ammonium formate;

[0058] f) sodium hydroxide;

[0059] g) Standard working solution:

[0060] 1) Standard substance solution: According to the purity of the standard substance, weigh an appropriate amount (among the standard substances of 13 kinds of shellfish toxins, the standard substance of C1 and C2 two kinds of toxins is C1 / C2 mixed standard substance, GTX1 and GTX4 two kinds of toxins The standard substance of GTX1 / GTX4 is mixed standard substance, the standard substance of GTX2 and GTX3 is GT...

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Abstract

The invention discloses a method for detecting 13 saxitoxins in a biological sample. The method comprises the following steps of performing extraction treatment on a biological sample to obtain a sample extracting solution; by taking water as a solvent, respectively preparing standard substance solutions by using standard substances of 13 saxitoxins, namely C1, C2, GTX1, GTX2, GTX3, GTX4, GTX5, STX, NEO, dcSTX, dcNEO, dcGTX2 and dcGTX3; respectively preparing standard solutions by using the standard substance solutions of the 13 saxitoxins; respectively preparing a mixed standard working solution by using standard substance solutions of 13 saxitoxins; preparing a quality control sample; respectively sucking the sample extracting solution, the blank sample extracting solution, the added sample extracting solution, the standard solution and the mixed standard working solution, detecting by adopting a liquid chromatography-mass spectrometer, analyzing and calculating a detection result. The method has advantages of being simple in determination method, easy to operate, good in repeatability, high in sensitivity and the like, is suitable for the field of forensic science, and realizes high-sensitivity simultaneous determination of multiple saxitoxins in a biological sample.

Description

technical field [0001] The invention relates to the technical field of shellfish toxin detection. Specifically, it is a test method for 13 shellfish toxins in biological samples. Background technique [0002] Toxins produced by poisonous algae often cause human poisoning through shellfish vectors, so such toxins are often referred to as shellfish poisons. Shellfish toxins are mainly divided into 5 categories: diarrheal shellfish poison DSP, paralytic shellfish poison PSP, neurological shellfish poison NSP, memory-impairing shellfish poison ASP, and citrate toxin CTX. Among them, the main manifestations of paralytic shellfish poisoning include: mild poisoning, the symptoms mainly manifest as paresthesia and numbness of the lips, mouth and tongue, and then spread to the face and neck, fingertips often have needle-like pain accompanied by slight Headache and dizziness. Slightly severe poisoning, paralysis of arms and legs, disturbance of voluntary movement, incoherent speech...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N30/04G01N2030/042
Inventor 栾玉静王芳琳黄健董颖崔冠峰接强李少元孙晓宇
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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