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Method for determining cell active oxygen and reduced glutathione simultaneouslly

A technique for glutathione and cell activity, which is applied in the detection field of simultaneous determination of cellular reactive oxygen species and reduced glutathione, can solve problems that have not been reported, and achieve convenient and operable experimental operation Effect

Inactive Publication Date: 2007-04-04
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

Simultaneous determination of cellular reactive oxygen species and reduced glutathione using a microfluidic chip has not been reported at home and abroad

Method used

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  • Method for determining cell active oxygen and reduced glutathione simultaneouslly
  • Method for determining cell active oxygen and reduced glutathione simultaneouslly
  • Method for determining cell active oxygen and reduced glutathione simultaneouslly

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Experimental program
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Embodiment

[0018] 1. Microfluidic chip design:

[0019] The design of the microfluidic chip used in the implementation of the present invention is shown in Figure 1 below. The glass chip used is a double T-shaped structure. The four round holes in the figure represent different solution pools, of which 2 is the buffer injection pool and 4 is the buffer. 1 is the sample pool, 3 is the sample waste pool, and 5 is the detection point. The separation channel of the chip is 8.5cm long (0.5cm from the buffer pool to the cross, and 8.0cm from the cross to the separation waste pool), and the sampling channel is 1.0cm long (from the sample pool to the cross and From the intersection to the sample waste pool is 0.5cm), the cross-section of the channel is approximately semi-elliptical, with an upper width of 50 μm, and the distance between the detection point and the intersection is 1.0cm.

[0020] 2. Microfluidic chip laser-induced fluorescence detector:

[0021] The laser excitation wavelength ...

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Abstract

A method for detecting cell active oxygen and reduced glutathion includes utilizing microfluid control chip to carry out detection of cell active oxygen and reduced plutathion simultaneously, setting buff solution concentration in electrophoresis of said chip to be 20-50mM and buffer solution pH value to be 8.0-10.1 as well as separation voltage to be 200-450v / cm and setting distance of detection point to cross point of sample feeding channel and separation channel to be 1.0-3.0cm.

Description

technical field [0001] The invention relates to the detection of intracellular active oxygen and reduced glutathione, in particular to a detection method for simultaneously measuring cellular active oxygen and reduced glutathione. Background technique [0002] Reactive oxygen species (ROS) and reduced glutathione (GSH) are two important signaling molecules in the process of intracellular signal transduction, which are closely related to the intracellular redox state. Active oxygen mainly refers to a class of oxygen molecules with high biological activity produced by exogenous oxidants or intracellular aerobic metabolism, such as O 2- , NO, H 2 o 2 , OH, etc. When the body is in a state of stress, cell hypoxia, damage or apoptosis, intracellular reactive oxygen species are excessively produced. Reduced glutathione is a strong reducing agent with the most content in cells. Its main function is to remove excess reactive oxygen species in the body, prevent protein sulfhydryl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N21/64G01N33/68
Inventor 秦建华叶囡楠姜雷谢敏豪林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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