85 results about "Intracellular reactive oxygen species" patented technology

Though copper is elevated in the tumor tissue and plasma of patients with various malignancies, the molecular targets for copper binding agents in angiogenesis and tumor progression remain poorly understood. It is disclosed that one anti-angiogenic target for the copper binding agent tetrathiomolybdate is intracellular CuZn-superoxide dismutase (SOD1). A second generation tetrathiomolybdate analog, ATN-224, inhibits endothelial cell (EC) proliferation in vitro, binds to SOD1 and inhibits its activity without displacing bound copper ATN-224 can accumulate in ECs and inhibit CuZnSOD activity with an IC₅₀ similar to the IC₅₀ for EC proliferation, resulting in increased generation of intracellular reactive oxygen species. Inhibition of EC proliferation by ATN-224 in vitro is substantially reversed by a synthetic porphyrin SOD mimetic. Similar results were observed in vivo, where inhibition of angiogenesis by ATN-224 in a Matrigel plug model was also reversed by MnTBAP. Thus, a distinct molecular target for copper depletion therapy has been identified and SOD1 is now validated as a target for anti-angiogenesis. Methods for screening, or designing, such SOD1 inhibitors for use as angiogenesis inhibitors and anti-cancer agents are disclosed.

The invention provides a microfluidic unicellular active oxygen automatic analyzer. The microfluidic unicellular active oxygen automatic analyzer consists of a system control module, a cell fluorescence mark module, a cell/fluid electric control module, a cell/fluid hydraulic control module, a microfluidic chip, a chip operation platform, a unicellular capture 'identifying and triggering' module,a fluorescence detection module, a signal processing and data acquiring module and program software. The microfluidic unicellular active oxygen automatic analyzer integrates a unicellular component analyzing method, cell fluorescence marking, cell/fluid control, fluorescence detection and a microelectronic control technique, provides a unicellular capture 'identifying and triggering' concept, identifies unicellular capture according to resistance change when a single cell reaches the inlet of a separation channel, and triggers automatic switching and data acquisition of 'unicellular capture and film dissolution' operation at the same time. The microfluidic unicellular active oxygen automatic analyzer can realize operation of cell fluorescence marking, cell sampling, unicellular capture, film dissolution, electrophoretic separation, unicellular active oxygen detection and the like on the microfluidic chip.

The invention discloses a method for detecting content of active oxygen in cells. The method has the advantage that a DCF (dichlorofluorescein) fluorescence signal value of a single live cell corresponding to pollutants exposed under different concentrations can be obtained; a detection result is accurate, and the problem of inaccurate measuring of final fluorescence intensity due to change of cell number caused by exposing of the pollutants is solved; the corresponding fluorescence signal value of single cell under a certain concentration can be truly reflected, and the injury to cells by pollutant exposing can be truly and accurately reflected, so as to lay a solid foundation for the application of an in-vitro cell model into the toxicity evaluation of the pollutants.

The invention discloses researches on mechanism of action of polyether compounds in anti-tumor. The drug is used for acting on at least one of the following signal pathways through induced apoptosis by affecting calcium, mediated apoptosis by affecting intracellular reactive oxygen species (ROS) level and mediated apoptosis by affecting cytochrome C level of cell mitochondria. The polyether compounds can cause death of tumor cells through controlling multiple signal pathways, and thus, the anticancer effects can be better achieved.

The invention discloses a pair of eudesmane sesquiterpene lactone epimers (1S,4S,5S,6S,7S,8S,9R,10S)-1,9-diacetoxy-4-hydroxy-6-isobutyryloxyprostatolide and (1S,4S,5R,6S,7S,8S,9R,10S)-1,9-diacetoxy-4-hydroxyl-6-isobutyryloxyprostatolide, pharmaceutically acceptable salts or solvates of the epimers, and pharmacodynamic activity of a pharmaceutical composition composed of the compounds, and mainly relates to a medical application of an anti-breast cancer medicine in inducing breast cancer cell MCF-7 cell morphology to retract and become round and the level of intracellular reactive oxygen species (ROS) to rise to show tumor cytotoxicity.

The invention discloses a synthesis method and application of a ruthenium (II) polypyridine metal complex. According to the ruthenium (II) complex, 2-(4-(thiophene-2-ethynyl) phenyl)-1H-imidazo [4, 5-f] [1, 10] phenanthroline is used as a main ligand, and 4, 4 '-dimethyl-2, 2'-dipyridyl is used as an auxiliary ligand. The synthesized complex can effectively inhibit proliferation of various tumor cells (A549, HepG-2, SGC-7901 and Hela), in addition, the ruthenium metal complex can effectively inhibit migration of the tumor cells, the content of active oxygen in the cells is remarkably increased, and the ruthenium metal complex is expected to be developed into a medicine for treating various tumors.

This invention relates to a method for separating blood cells comprising the steps of: (a) lysing red blood cells of a blood sample and diluting said sample; (b) providing a microfluidic device comprising a spiral-shaped flow channel having at least a first end and a second end, wherein said flow channel has two inlet ports at or near said first end and at least two outlet ports at or near said second end, wherein one of the two inlet ports is located at the inner wall of the spiral-shaped flow channel and the other inlet port is located at the outer wall of the spiral-shaped flow channel and at least one of the outlet ports is connected to a container allowing the storage of blood cells; (c) introducing the sample of step (a) into the inlet port located at the outer wall of the spiral-shaped flow channel and introducing a sheath fluid into the inlet port located at the inner wall of the spiral-shaped flow channel; (d) driving said sample and the sheath fluid through the spiral-shaped flow channel; and (e) recovering the blood cell in the at least one container connected to the at least one outlet port. The present invention also relates to coupling above described method of purifying neutrophils in the native state with a method for diagnosing diabetes or an inflammatory disease in a subject, which involves further investigation of neutrophils by determining their rolling speed, the neutrophil circularity (NC) index and/or the expression of markers, such as intracellular reactive oxygen species (ROS), CD1 1 b or PSGL-1.

The invention relates to a method capable of remarkably improving stress resistance of eggplants under low-temperature and weak-light conditions. The method comprises the following steps: (1) plump eggplant seeds are selected, disinfected with Na3PO4 solution and soaked for germination acceleration, and sowing is performed after the seeds sprout; (2) after three true leaves grow out from each eggplant seedling, a 50-250 mu mol.L<1> melatonin solution is adopted to spray the leaves and applied once every 2-3 days, 3-5 mL of the melatonin solution is sprayed to each eggplant seedling and the melatonin solution is sprayed 3-4 times totally. With the adoption of the method, damage of low temperature and weak light to the eggplants can be relieved, the photosynthesis is improved, the activity of in-vivo antioxidase including POD (peroxidase), CAT (catalase) and SOD (superoxide dismutase) in the eggplants is improved, the content of MDA (methane dicarboxylic aldehyde) is reduced, metabolism balance of intracellular reactive oxygen species is maintained, damage to cell membranes is avoided, accumulation of proline and soluble sugar is accelerated, the eggplants can maintain relatively high solar energy capturing efficiency under the weak-light condition, and the resistance of the eggplant seedlings to low temperature and weak light is improved.

A method for treating symptoms of a lipotoxicity-related phenomenon includes introducing into a patient a therapeutically effective synergistic amount of a phenomenon-inhibiting cocktail of a polyphenol (P), thiol antioxidant (To) and ascorbic acid (A) to reduce intracellular reactive oxygen species (ROS) and/or increase intracellular nitrite (NO), so that reduction of such phenomenon is at least 30% greater than the reduction of such phenomenon provided by separate administration of each of P, To and A.

The invention provides application of Nifeviroc in preparing an antitumor drug, and provides an antitumor drug containing Nifeviroc. As is proved by cell experiments, after Nifeviroc acts on tumor cells, the cell cycle progress can be interfered, and the cell growth is inhibited, so that the cell cycle is stagnant at the G0-G1 stage; meanwhile, cell apoptosis can also be induced by increasing theactive oxygen content in the cells and lowering the mitochondrial membrane potential; meanwhile, Nifeviroc has an obvious effect of inhibiting invasion and metastasis of the tumor cells, by inhibitingadhesive power of the tumor cells to an extracellular matrix, the directed migration capability, the non-targeted migration capability and the in-vitro invasion capability of the cells are lowered, and the increase in size of the tumors in vivo can also be inhibited. As can be seen, Nifeviroc has a good effect of resisting the activity of the tumor, can be used for preparing the antitumor drug, does not have an obvious side effect, and has a good application prospect.

The present application provides the use of hyaluronic acid or a salt thereof and/or trehalose for stabilizing ergothioneine, and an additive for stabilizing ergothioneine and comprising hyaluronic acid or a salt thereof and/or trehalose and comprising ergothioneine, hyaluronic acid or a salt thereof and trehalose. Compared with other auxiliary materials, the hyaluronic acid or the salt thereof and the trehalose provided by the invention can obviously relieve the damage of high temperature to ergothioneine and improve the yield of ergothioneine powder products. The composition disclosed by theinvention has a relatively good antioxidant effect and an anti-apoptosis effect. Meanwhile, the inhibiting and scavenging effects of the composition obtained by compounding hyaluronic acid or salt thereof and trehalose as auxiliary materials on reactive oxygen free radicals in cells are obviously higher than those of the composition obtained by using single hyaluronic acid or salt thereof or trehalose as an auxiliary material.

The invention belongs to the technical field of plant molecular biology, and in particular discloses a shepherd's purse peroxidase gene promoter and an application thereof in improving plant cold tolerance. The nucleotide sequence of the shepherd's purse peroxidase gene promoter is that of the 1st-1021st bases in SEQ ID No.1. A gene driven by the promoter can code a peroxidase gene which is inducible by low temperature and is capable of regulating reactive oxygen in plant cells. Under an induction condition of 4 DEG C, the expression amount of the promoter is up-regulated, and meanwhile, the expression amount of the promoter is also up-regulated in leaves and stems. An inducible plant expression vector, which is constructed by virtue of promoter fragments, can start the expression of a target gene in the case that plants are under low-temperature stress. The invention also provides an application of the promoter in breeding cold-tolerance plants, so that the cold tolerance of crop varieties can be improved.

The invention discloses serial water-soluble hydroxycamptothecine naphthenic amino alcoholderivative. The serial water-soluble hydroxycamptothecine naphthenic amino alcohol derivative is synthesized by leading naphthenic amino alcohol methylene into a hydroxycamptothecine structure for the first time; and the hydroxycamptothecine with strong cell toxicity is decorated into the hydroxycamptothecine naphthenic amino alcohol derivative with medium cell toxicity. Therefore, the selectivity of induction of tumor cell apoptosis is improved; the situation that the hydroxycamptothecine prolinol has special hydrogenation reduction metabolic pathway in the body is found out for the first time; existing camptothecin derivatives do not have special hydrogenation reduction metabolic pathway; and active oxygen in the cell can be controlled, so as to inhibit cell proliferation, selectively induce cancer cell apoptosis, and inhibit cell inflammatory necrosis. Normal cell function is not affected.

The invention provides a PDK1 inhibitor, a composition containing the inhibitor and application, belonging to the technical field of medicine. Specifically speaking, bishydroxycoumarin enables cell p-PDH expression to be reduced through inhibiting PDK1 activity in ovarian cancer cells, promotes cellular tricarboxylic acid cycle, increases reactive oxygen species in cells, promotes mitochondrial membrane potential to be reduced, and enables mitochondrial membrane permeability to be lowered and cytochrome C to be increased, thereby causing a cancer cell apoptosis mechanism to realize application in preparation of medicines for treating and/or preventing ovarian cancer. In addition, a pharmaceutical composition which takes the bishydroxycoumarin as an active ingredient has an obvious inhibiting effect on the tumor volume size of the ovarian cancer. The bishydroxycoumarin and bishydroxycoumarin compounds provided by the application not only have an ovarian cancer treating effect through inhibiting PDK1, but also cannot cause peripheral nervous toxicity, provide new thoughts and ways for treating the ovarian cancer, and have broad application prospects and clinical values.

The invention discloses a method for evaluating the influences of smoking on the quality of oocytes. Specifically, oocytes are cultured to be mature in culture solutions containing different concentrations of cotinine. Finally, the influences of smoking on the quality of oocytes are evaluated by detecting some indicators, such as the maturation rate of oocytes, chromosomal aneuploidy, actin distribution, mitochondrial membrane potential, intracellular reactive oxygen species level, spindle morphology, chromosome arrangement of mature oocytes, sperm binding number after in-vitro fertilization,pronucleus formation rate after parthenogenetic activation and the like. Through a large number of objective tests, the influences of smoking on the quality of oocytes are evaluated, thus providing areference of theoretical data for the maintenance of reproductive capacity of female smokers.

The invention discloses that tryptophan has the effect of enhancing the bactericidal effect on gram-negative bacteria, and particularly relates to application of tryptophan to preparation of bactericidal medicines for gram-negative bacteria. The design experiment proves that tryptophan can be added to gentamicin and kanamycin to increase the intracellular reactive oxygen species level of bacteriaand further increase the absorption of antibiotics by the bacteria, thereby improving the sensitivity of the bacteria to drugs. The trapping effect of tryptophan on the gram-negative bacteria and theimprovement of the bactericidal effect on drug-resistance bacteria are fully proved. Based on the above findings, secondary medical use of tryptophan, a bactericidal composition, a bactericidal drug and a bactericidal method are correspondingly disclosed.

The invention relates to the field of nanometer materials and particularly discloses application of a copper oxide-platinum nanocomposite in antibiosis. A large number of active oxygen free radicals are greatly generated by virtue of the common action of a copper oxide-platinum nanocomposite material and hydrogen peroxide; the active oxygen free radicals are adjusted to be in a steady state by virtue of enzyme (such as superoxide dismutase) and of small molecules (such as ascorbic acid) of an organism under a normal physiological state, and the harm caused to the organism is avoided; and once the steady state is broken, the content of the active oxygen free radicals in cells is increased to cause oxidative stress disorder in bacteria, so as to achieve a bacteriostasis purpose. According to the copper oxide-platinum nanocomposite, the growth of the bacteria can be effectively inhibited, and bacterial biofilms can be cleared.

The invention discloses an application of nano selenium as an X-ray radiation therapy sensitizer. In the invention, elementary selenium is dissolved in PEG to prepare the nano selenium (SeNPs), nano selenium modified by polysaccharide and chitosan and tumor-targeted nano selenium modified by folic acid and transferrin. The SeNPs has an X-ray stimulating and responding character and has a synergistic effect with the X-ray, thereby high-effectively inhibiting proliferations of human cervical carcinoma cell Hela, melanoma cell A375, osteosarcoma cell MG-63, breast cancer cell MCF-7 and brain glioma cell C6 and the like. The main mechanism of the SeNPs is that by means of increase of active oxygen accumulation in cells, further cell period retardant tumor cell apoptosis is induced. A result proves that the SeNPs can be developed as a novel radiation therapy sensitizer.