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Composition of hyaluronic acid or salt and/or trehalose thereof and use thereof for stabilizing ergothioneine

A technology of hyaluronic acid and ergothioneine, which is applied in the direction of drug combination, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems affecting the yield of powder products, ergothioneine damage, etc., and achieve Excellent anti-oxidation effect, alleviating damage, excellent effect

Active Publication Date: 2021-02-12
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the existing spray drying method dries ergothioneine, ergothioneine is easily destroyed, which affects the yield of the powder product obtained by drying ergothioneine

Method used

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  • Composition of hyaluronic acid or salt and/or trehalose thereof and use thereof for stabilizing ergothioneine
  • Composition of hyaluronic acid or salt and/or trehalose thereof and use thereof for stabilizing ergothioneine
  • Composition of hyaluronic acid or salt and/or trehalose thereof and use thereof for stabilizing ergothioneine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The optimization (matsu mushroom fermentation) of embodiment 1 ergothioneine composition preparation

[0043] Liquid seed medium: 3.0% (w / v) lactose, 2.0% (w / v) potato extract powder, 0.1% (w / v) dipotassium hydrogen phosphate, 0.1% (w / v) sodium sulfate , the rest is water, pH 4.0-4.5, sterilized at 121°C for 20 minutes;

[0044] Fermentation medium: 3.0% (w / v) glucose, 2.0% (w / v) maltose, 1.0% (w / v) beef extract, 1.5% (w / v) tryptone, 0.06% (w / v) / v) sodium dihydrogen phosphate, 0.0003% (w / v) zinc chloride, 0.0005% (w / v) niacin, 0.0001% (w / v) vitamin B 1 , the rest is water, pH 4.0-4.5, sterilized at 121°C for 20min.

[0045] Tricholoma Matsutake (Tricholoma Matsutake) CCTCC No: M 2020587 mycelium slants were inoculated into the liquid seed medium, and cultured for 25 days at 23° C. and 200 rpm shaking conditions to obtain Tricholoma Matsutake seed liquid. Insert the seed liquid into the fermentation medium with an inoculation amount of 5% by volume, and culture it fo...

Embodiment 2

[0051] The optimization (Hericium Hericium fermentation) of embodiment 2 ergothioneine compositions preparation

[0052] Liquid seed medium: 4.0% (w / v) sucrose, 1.5% (w / v) soybean cake powder, 0.2% (w / v) sodium dihydrogen phosphate, 0.1% (w / v) sodium sulfate, The rest is water, pH 4.0-4.5, sterilized at 121°C for 20 minutes;

[0053] Fermentation medium: 3.0% (w / v) glucose, 1.5% (w / v) beef extract, 0.05% (w / v) sodium dihydrogen phosphate, 0.03% (w / v) sodium sulfate, 0.0003% Zinc Chloride (w / v), Niacin 0.0006% (w / v), Vitamin B 0.0001% (w / v) 1 , the rest is water, pH 4.0-4.5, sterilized at 121°C for 20min.

[0054] Hericium erinaceus (Hericium erinaceus) CCTCC No: M 2018567 mycelium slant strain was inoculated into the liquid seed medium, and cultured for 7 days at 23° C. and 200 rpm shaking conditions to obtain Hericium erinaceus seed liquid. The seed solution was inserted into the fermentation medium with an inoculation amount of 5% by volume, and cultured for 10 days at 23...

Embodiment 3

[0059] Example 3 Optimization of the ratio of hyaluronic acid and trehalose

[0060] From the results of Example 1 and Example 2, it can be seen that sodium hyaluronate and trehalose have good stabilizing effects on ergothioneine. Among them, sodium hyaluronate has good moisturizing and anti-inflammatory effects, but the price is relatively high. Therefore, in order to reduce costs and obtain a composition with good moisturizing and anti-inflammatory effects at the same time, it is considered to use hyaluronic acid Sodium (5KDa) and trehalose in different proportions are used as spray auxiliary materials. Wherein, using the fermented liquid of Example 1, when the fermented liquid is spray-dried, a mixture of sodium hyaluronate and trehalose is used as an auxiliary material, wherein the mass ratio of sodium hyaluronate and trehalose is 1:99-50 : Adjust between 50. Also according to the adjuvant add-on of fermented liquid 5% (w / v, g / ml), use the above-mentioned same spray-dryi...

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Abstract

The present application provides the use of hyaluronic acid or a salt thereof and / or trehalose for stabilizing ergothioneine, and an additive for stabilizing ergothioneine and comprising hyaluronic acid or a salt thereof and / or trehalose and comprising ergothioneine, hyaluronic acid or a salt thereof and trehalose. Compared with other auxiliary materials, the hyaluronic acid or the salt thereof and the trehalose provided by the invention can obviously relieve the damage of high temperature to ergothioneine and improve the yield of ergothioneine powder products. The composition disclosed by theinvention has a relatively good antioxidant effect and an anti-apoptosis effect. Meanwhile, the inhibiting and scavenging effects of the composition obtained by compounding hyaluronic acid or salt thereof and trehalose as auxiliary materials on reactive oxygen free radicals in cells are obviously higher than those of the composition obtained by using single hyaluronic acid or salt thereof or trehalose as an auxiliary material.

Description

technical field [0001] The application relates to the field of biochemical technology, in particular to a use of hyaluronic acid or its salt and / or trehalose in stabilizing ergothioneine, an ergothioneine composition containing hyaluronic acid or its salt and / or trehalose . Background technique [0002] Ergothioneine (2-mercaptohistidine trimethylbetaine) is a rare amino acid that has the functions of anti-oxidation, UV protection and cell repair. Ergothioneine exists in many animals and plants, cannot be synthesized by the animal body itself, and can only be ingested from food. There are three methods for preparing ergothioneine: chemical synthesis, extraction and microbial fermentation. Due to the problems of high cost and unsafety in the preparation of ergothioneine by chemical synthesis and extraction, more and more attention has been paid to the preparation of ergothioneine by microbial fermentation. [0003] When using microbial fermentation to produce ergothioneine...

Claims

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Application Information

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IPC IPC(8): A61K31/4172A61K47/36A61K47/26A61P39/06A61P17/16
CPCA61K31/4172A61K47/36A61K47/26A61P39/06A61P17/16A61K9/1652A61K9/1623A23L33/175A23L31/15A61K8/735A61K8/60A61K8/4946A61Q17/04A61K2800/522C12N1/14C12N2500/34
Inventor 魏玉洁陆震陈清平李杰孙元军谢文娟杜冉冉郭文逸郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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