Microfluidic unicellular active oxygen automatic analyzer

Abstract

The invention provides a microfluidic unicellular active oxygen automatic analyzer. The microfluidic unicellular active oxygen automatic analyzer consists of a system control module, a cell fluorescence mark module, a cell/fluid electric control module, a cell/fluid hydraulic control module, a microfluidic chip, a chip operation platform, a unicellular capture 'identifying and triggering' module,a fluorescence detection module, a signal processing and data acquiring module and program software. The microfluidic unicellular active oxygen automatic analyzer integrates a unicellular component analyzing method, cell fluorescence marking, cell/fluid control, fluorescence detection and a microelectronic control technique, provides a unicellular capture 'identifying and triggering' concept, identifies unicellular capture according to resistance change when a single cell reaches the inlet of a separation channel, and triggers automatic switching and data acquisition of 'unicellular capture and film dissolution' operation at the same time. The microfluidic unicellular active oxygen automatic analyzer can realize operation of cell fluorescence marking, cell sampling, unicellular capture, film dissolution, electrophoretic separation, unicellular active oxygen detection and the like on the microfluidic chip.

Description

Technical field: [0001] The invention relates to microfluidic analysis platform technology, in particular to a microfluidic unit that can automatically realize cell fluorescence labeling on a microfluidic chip, cell loading, single cell capture, membrane dissolution, electrophoretic separation and intracellular active oxygen detection. Cell active oxygen automatic analyzer. Background technique: [0002] Cells are the basic unit of the morphological structure and life activities of organisms. Various reactive oxygen species (ROS), such as superoxide anion (O 2 - ), hydrogen peroxide (H 2 o 2 ), hydroxyl radicals (OH·), lipid radicals (ROO·), peroxynitroso anions (ONOO - )Wait. Appropriate levels of reactive oxygen species are important to normal physiological processes of organisms, and they participate in cell signal transduction, cell growth regulation, synthesis of important biological substances, and cell metabolism. When organisms are under oxidative stress, exog...

AI Technical Summary

Problems solved by technology

The advantages of laser scanning confocal microscopy and scanning tunneling microscopy are that they can observe and analyze reactive oxygen species in situ, in real time, and in space; their disadvantages are: limited by factors such as high quality limit and low resolution , it is difficult to quantitatively detect the content of reactive oxygen species in single cells

Its disadvantages are: since its measurement data are relative values ​​compared with biological samples, the non-specific binding of antibodies directly affects the definition of positive and negative cells, so the system needs to be calibrated or calibrated before use, which has cumbersome operations and cell consumption. Problems such as large size, bulky equipment, and high price

At present, the main technical problems faced by microfluidic single-cell component analysis are: 1) lack of means to effectively manipulate single cells; 2) lack of adaptive technology to automatically know that a single target cell has been captured; 3) detection sensitivity needs to be further improved Improvement; 4) Lack of instrument coupling technology that automatically realizes the entire analysis process

However, due to the above-mentioned technical bottlenecks and the limitation of on-chip cellular ROS labeling technology, there is no microfluidic analysis instrument capable of detecting ROS in a single cell.

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