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Application of copper oxide-platinum nanocomposite in antibiosis

A technology of platinum nanometer and copper oxide, which is applied in the direction of antibacterial drugs, active ingredients of peroxides, and resistance to media-borne diseases. Effect

Active Publication Date: 2017-05-10
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been found through experiments that it has a certain antibacterial effect on bacteria (such as Escherichia coli and Staphylococcus aureus), but the antibacterial effect is not very satisfactory

Method used

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  • Application of copper oxide-platinum nanocomposite in antibiosis
  • Application of copper oxide-platinum nanocomposite in antibiosis
  • Application of copper oxide-platinum nanocomposite in antibiosis

Examples

Experimental program
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Effect test

preparation example Construction

[0029] The preparation method of the bacterial suspension in the following examples is as follows: using an inoculation loop to inoculate two kinds of bacteria in LB medium by aseptic operation, and cultivate overnight at 37° C. and 150 rpm.

[0030] The cultivation method of the bacterial biofilm in the following examples is as follows: (1) Add 900 μL of TSB medium into a 24-well plate through aseptic operation. (2) Add 100 μL of bacteria in logarithmic growth phase to each well. (3) Incubate the 24-well plate in an incubator for 48 hours at 37°C, and replace with fresh TSB medium every 12 hours.

[0031] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0032] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

Embodiment 1

[0033] The preparation of embodiment 1 copper oxide-platinum nanocomposite

[0034] 1. Preparation of copper oxide nanosheets

[0035] Dissolve 0.5g copper chloride dihydrate and 0.5g CTAB in 15mL water, and add 1mL sodium hydroxide solution (0.3g / mL); transfer the above reaction solution into a 20mL reactor, and react at 120°C for 6h. After the reaction is finished, the copper oxide nanosheets are obtained by centrifugation and washing.

[0036] 2. Preparation of copper oxide-platinum nanocomposites

[0037] First, 660 μL of copper oxide nanosheets (170 mg / L) was added into 2.34 mL of ultrapure water, and ultrasonicated for 10 min to make them uniformly dispersed. Then, 23.5 μL of H 2 PtCl 6 ·6H 2 O in water (19.3 mM). Finally, under an ice bath, stir rapidly and add 1 mL of NaBH dropwise 4 Aqueous solution (4.8mM), continue to stir for 2h after the dropwise addition. After the reaction is finished, the copper oxide-platinum nanocomposite is obtained through centrifug...

Embodiment 2

[0038] Example 2 Application of Copper Oxide-Platinum Nanocomposite in Antibacterial

[0039] 1. Measurement of reactive oxygen species in bacterial cells by copper oxide-platinum nanocomposites

[0040] Step: (1) collect the bacterium of logarithmic growth phase, wash with the PBS of 0.01M, and adjust its OD600=0.1 (10 8CFU / mL), aliquot 20 μL into a 1.5 mL centrifuge tube. (2) Add copper oxide-platinum nanocomposites to the centrifuge tube at a final concentration of 20 μg / mL, and simultaneously add hydrogen peroxide. The final concentration of hydrogen peroxide for Escherichia coli is (50 μM), and the final concentration of hydrogen peroxide for Staphylococcus aureus is (300 μM). (3) Place the above-mentioned centrifuge tube in an incubator at 37° C. and 150 rpm for 3 hours. (4) Add fluorescent probe DCFH-DA (100 μM) to the above system, incubate at 37° C., 150 rpm for 30 minutes. (5) Use a microplate reader to measure the fluorescence value of excitation at 485nm and em...

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Abstract

The invention relates to the field of nanometer materials and particularly discloses application of a copper oxide-platinum nanocomposite in antibiosis. A large number of active oxygen free radicals are greatly generated by virtue of the common action of a copper oxide-platinum nanocomposite material and hydrogen peroxide; the active oxygen free radicals are adjusted to be in a steady state by virtue of enzyme (such as superoxide dismutase) and of small molecules (such as ascorbic acid) of an organism under a normal physiological state, and the harm caused to the organism is avoided; and once the steady state is broken, the content of the active oxygen free radicals in cells is increased to cause oxidative stress disorder in bacteria, so as to achieve a bacteriostasis purpose. According to the copper oxide-platinum nanocomposite, the growth of the bacteria can be effectively inhibited, and bacterial biofilms can be cleared.

Description

technical field [0001] The invention relates to the field of nanomaterials, in particular to the application of a copper oxide-platinum nanocomposite capable of efficiently generating active oxygen free radicals in antibacterial aspects. Background technique [0002] Diseases caused by bacterial infections have increasingly become one of the chief culprits threatening human health. Such as: cholera, pneumonia, malaria, tuberculosis and hepatitis, etc., are all caused by the spread of bacteria or microorganisms. In biomedical implants, surgical equipment, industrial pipelines and other instruments, bacterial colonies and biofilms are the main causes of some infectious disease outbreaks and hospital-acquired infections. Bacterial colonies and biofilms can form a protective barrier around the periphery, preventing traditional antimicrobial therapy and immune responses from completely eradicating these pathogenic foci. The advent of antibiotics provides an effective treatment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/40A61P31/04A61K33/34A61K33/24
CPCA61K33/24A61K33/34A61K33/40A61K2300/00Y02A50/30
Inventor 贾星航杨蓉王新环王琛
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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