Antioxidant, Anti-inflammatory or Anti-aging composition containing taxus cambium- or procambium-derived cell line as active ingredient

a technology of anti-inflammatory and anti-aging compositions, applied in hair cosmetics, antinoxious agents, metabolic disorders, etc., can solve the problems of reducing adaptability, reducing metabolic rate, and death of cells and the whole of tissues of the human body

Active Publication Date: 2011-05-19
WELLKEY HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014]It is an object of the present invention to a natural material-derived composition which has minimized side effects compared to existing antioxidants and anti-inflammatory agents and shows antioxidant and anti-inflammatory activities and the effects of preventing and delaying aging.

Problems solved by technology

Aging occurs throughout the cells and tissues of the human body, displays a decrease in metabolic rate, an increase in diseases, a decrease in adaptability and the like, and ultimately leads to the death of the cells and the whole of tissues of the human body.
The wear-and-tear theories suggest that the cells of organisms lose their functions with the passage of time or due to the accumulation of harmful substances.
Free radicals are substances having one unpaired electron in the outermost orbit and their structure is highly unstable and reactive because they tend to be stabilized by receiving electrons.
Particularly, free radicals which are derived from oxygen are termed “reactive oxygen species”, and these reactive oxygen species react with proteins, lipids, carbohydrates and the like to cause lipid peroxidation, DNA damage, protein oxidation and the like, thereby causing damage intracellular structures and ultimately causing the death of cells.
Aged cells which are in the progression of replicative senescence can produce ROS at higher levels compared to young cells and also produce toxic byproducts, such as superoxide, hydrogen peroxide, hydroxyl radicals and the like during a normal metabolic process.
However, the antioxidant enzymes show a decrease in the defense ability against reactive oxygen species as people get older, and the synthetic antioxidants are known to have mutagenecity and toxicity.
For normal persons, inflammatory responses remove external sources of infection, reproduce damaged tissues, and recover the function of organisms, but when an antigen is not removed or inflammatory responses occur excessively or continuously due to intrinsic substances, inflammatory responses stimulate damage to mucosa and, as a result, in some cases, cause diseases such as cancer.

Method used

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  • Antioxidant, Anti-inflammatory or Anti-aging composition containing taxus cambium- or procambium-derived cell line as active ingredient
  • Antioxidant, Anti-inflammatory or Anti-aging composition containing taxus cambium- or procambium-derived cell line as active ingredient
  • Antioxidant, Anti-inflammatory or Anti-aging composition containing taxus cambium- or procambium-derived cell line as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Taxus Cambium- or Procambium-Derived Cell Line

[0064](1) Preparation of Plant Material

[0065]Each of the twig and stem of Taxus was collected, and then immediately soaked in 100 mg / L of the antioxidant L-ascorbic acid (DUCHEFA, The Netherlands). Then, they were transported and stored.

[0066]Then, the plant was pretreated with a mixed solution of 1% benomyl (Dongbu Hannong Chemical, Korea), 1% daconil (Dongbu Hannong Chemical, Korea), 1% sterptomycin sulphate (DUCHEFA, The Netherlands) and 0.1% cefotaxime sodium (DUCHEFA, The Netherlands) for 24 hours, and then washed with tap water for 30 minutes to remove phenolic compounds and the remaining chemicals. Next, the plant was surface-sterilized in 70% ethanol (DC Chemical, Korea) for 1 min, 30% hydrogen peroxide (LG Chemical, Korea) for 15 min, 1% CLOROX solution for 15 min and 3% CLOROX solution for 5 min, and then washed 3-4 times with water.

[0067](2) Isolation of Procambium and Cambium Tissues from Twig and Stem

[0068]The...

example 2

Preparation of Extract of Procambium- or Cambium-Derived Cell Line

[0081](1) Preparation of DMSO (dimethyl sulfoxide) Extract

[0082](i) 500 g of the cell line from which the medium has been removed were dissolved in 500 ml of DMSO with stirring at 50° C. for 6 hours.

[0083](ii) After completion of the dissolution, the cell solution was centrifuged at 3,000 g for 10 minutes, and the supernatant was collected, thus obtaining a DMSO-soluble substance.

[0084](iii) The obtained DMSO-soluble substance was concentrated using a rotary vacuum concentrator.

[0085](iv) The concentrated sample was dried using a freeze dryer, thereby obtaining a DMSO extract.

[0086](2) Preparation of Distilled Water Extract, Methanol Extract and Acetone Extract

[0087]From the cell line prepared in Example 1, active substances were extracted stepwise as follows (FIG. 2).

[0088](i) 500 g of the cell line from which the culture medium has been removed was dissolved in 500 ml of distilled water with stirring at 50° C. for 6...

example 3

Culture of Human Diploid Fibroblasts (HDF)

[0096]HDF cells were isolated from the fetal penis prepuce and cultured. The culture medium was prepared by adding 10% fetal bovine serum (FBS, Hyclone, Logan, Utah, USA) inactivated by heating at 56° C. for 30 minutes, 100 unit / ml of penicillin, 100 μg g / ml of streptomycin and 300 μg / ml of glutamine to DMEM medium (Invitroge Gibco life tech. Vienna, Austria). The cells were cultured in the medium, described above, in a 5% CO2 incubator at a temperature of 37° C. and a humidity of 95% and subcultured at 3-4-day intervals, immediately before the cells were fused with each other. The subcultured cells were divided, according to the number of subcultures (passages), into young cells cultured less than 20 passages, middle cells cultured for 21-49 passages, and aged cells cultured more than 50 passages. The cultured cells were used in the experiments of Examples 4 to 6.

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Abstract

The present invention relates to an antioxidant, anti-inflammatory or anti-aging composition containing any one or more of a Taxus cambium- or procambium-derived cell line, an extract thereof, a lysate thereof and a culture medium thereof. The composition according to the present invention has minimized side effects compared to existing antioxidants and anti-inflammatory agents, is involved in intracellular metabolism to reduce intracellular reactive oxygen species, and reduces and induces aging-related signals. Thus, the composition of the preset invention is useful for preventing and delaying aging. In addition, the composition of the present invention has the effect of inhibiting melanogenesis, and thus is useful as a whitening cosmetic composition.

Description

TECHNICAL FIELD[0001]The present invention relates to an antioxidant, anti-inflammatory or anti-aging composition containing any one or more of a Taxus cambium- or procambium-derived cell line, an extract thereof, a lysate thereof and a culture medium thereof.BACKGROUND ART[0002]Aging is a functional, structural and biochemical process that occurs continuously throughout the life of human beings. Aging occurs throughout the cells and tissues of the human body, displays a decrease in metabolic rate, an increase in diseases, a decrease in adaptability and the like, and ultimately leads to the death of the cells and the whole of tissues of the human body. Theories explaining the processes and causes of aging are broadly divided into the genetic theories (Chung. H. Y. et al., Kor. J. Gerontol., 2:1, 1992) and the wear-and-tear theories (Chung. H. Y. et al., Kor. J. Gerontol., 2:1, 1992). The wear-and-tear theories suggest that the cells of organisms lose their functions with the passage...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K8/97A61K36/13C12N5/04C12N5/02A61P29/00A61P39/06A61Q19/02A61Q19/08
CPCA61K8/97A61Q19/08A61Q19/02A61K36/13A61K8/9728A61K8/9755A61P3/00A61P17/00A61P17/18A61P29/00A61P39/06C12N5/04
Inventor LEE, DAE HEELEE, EUN KYONGSO, EUN MIJIN, YOUNG WOO
Owner WELLKEY HLDG LTD
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