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Method of analyzing heavy metal pollution of vegetable based on reactive oxygen species level

A technology of free radicals and heavy metals, which can be used in material excitation analysis, fluorescence/phosphorescence, etc., and can solve the problems of cumbersome analysis procedures and complex processing.

Inactive Publication Date: 2013-12-25
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Liquid chromatography, gas chromatography and spectroscopic techniques are routine analytical methods to analyze whether vegetables are contaminated, but these methods have complicated sample pretreatment and cumbersome analysis procedures

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1 Determination of heavy metal content by inductively coupled plasma mass spectrometer and standard vegetable setting: lead ion standard solution was purchased from the Institute of Chemical Ionization, Shanghai Institute of Metrology and Testing Technology, a lead standard solution with a known concentration of 1000 μg / mL was taken, and an appropriate amount of nitric acid was added , diluted with ultrapure water to prepare a gradient standard solution, and shaken to obtain a series of lead standard solutions.

[0016] The content of heavy metals was determined by inductively coupled plasma mass spectrometer (Agilent HP4500 Series 300, USA). By optimizing the working parameters of the inductively coupled plasma mass spectrometer, a quantitative analysis method for the content of lead in vegetable leaves was established.

[0017] Using inductively coupled plasma mass spectrometry to measure the content of lead ion in commercially available spinach, the content of lead io...

Embodiment 2

[0022] 1 Determination of heavy metal content by inductively coupled plasma mass spectrometer and standard vegetable setting: Mercury ion standard solution was purchased from the Chemical Ionization Institute of Shanghai Institute of Metrology and Testing Technology. , diluted with ultrapure water to prepare a gradient standard solution, and shake well to obtain a series of mercury standard solutions.

[0023] The content of heavy metals was determined by inductively coupled plasma mass spectrometer (Agilent HP4500 Series 300, USA). By optimizing the working parameters of the inductively coupled plasma mass spectrometer, a quantitative analysis method for mercury content in vegetable leaves was established.

[0024] The content of heavy metal mercury in commercially available spinach was measured by inductively coupled plasma mass spectrometry. The mercury ion content in three spinach samples was 2.26±0.31, 10.35±0.35, 20.58±0.62 μg / Kg, and the content of 2.26±0.31 μg / Kg was le...

Embodiment 3

[0029] 1 Determination of heavy metal content by inductively coupled plasma mass spectrometer and standard vegetable setting: cadmium ion standard solution was purchased from the Chemical Ionization Institute of Shanghai Institute of Metrology and Testing Technology. , diluted with ultra-pure water to prepare a gradient standard solution, and shake well to obtain a series of cadmium standard solutions.

[0030] The content of heavy metals was determined by inductively coupled plasma mass spectrometer (Agilent HP4500 Series 300, USA). By optimizing the working parameters of the inductively coupled plasma mass spectrometer, a quantitative analysis method for cadmium content in vegetable leaves was established.

[0031] The content of heavy metal cadmium in commercially available spinach was measured by inductively coupled plasma mass spectrometer. The contents of cadmium ions in three spinach were 5.76±0.25, 15.32±0.65, 25.67±0.43 μg / Kg, and the content was 5.76±0.25 μg / Kg. Nati...

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Abstract

The invention provides a method of evaluating the heavy metal pollution of vegetable based on reactive oxygen species level by analyzing the fluorescence intensity of reactive oxygen species in vegetable cells through adopting laser scanning confocal microscope technology. The method comprises the following steps: 1) marking reactive oxygen species in vegetable leaf cells by adopting 2',7'-dichorofluorescein diacetate (DCFH-DA); 2) analyzing the reactive oxygen species level in vegetable leaf cells by adopting the laser scanning confocal microscope technology; 3) fixing instrument acquisition parameters by taking the fluorescence intensity of reactive oxygen species in the non-polluted vegetable leaves according with the national standard; 4) further acquiring the fluorescence density value of the vegetable leaf to be detected according to the instrument parameter, performing statistic analysis on the fluorescence intensity value of vegetable to be detected and the standard vegetable fluorescence intensity value so as to confirm whether the vegetable is polluted by heavy metal.

Description

technical field [0001] The invention relates to a method for analyzing heavy metal pollution in vegetables based on the level of active oxygen free radicals, and belongs to the technical field of food detection. Background technique [0002] With the rapid development of industry and agriculture, the discharge of industrial "three wastes" and the extensive use of agricultural materials such as pesticides and fertilizers have caused vegetables to be polluted by heavy metals to varying degrees. Soil microorganisms cannot decompose heavy metals, heavy metals can be enriched in organisms, and some heavy metals will be transformed into more toxic methyl compounds in organisms. These pollutants may accumulate in the human body at harmful concentrations through the food chain, seriously endangering human health. . Therefore, it is necessary to analyze and evaluate heavy metals in vegetables, which is of great significance for ensuring the quality of vegetables and ensuring people'...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 刘东武陈志伟项海波
Owner SHANDONG UNIV OF TECH
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