Method for appraising salinity tolerance potential by using stress resistance of isolated leaves

Abstract

The invention discloses a method for appraising salinity tolerance potential by using stress resistance of isolated leaves. In the method, the leaves directly absorb NaCl, membrane permeability of leaf cells can be destroyed, and the magnitude of relative conductivity can precisely reflect the salinity tolerance of different poplar leaf cells. The membranes of poplar leaf cells are destroyed in high-salt condition, destruction degrees of cell membranes with different salinity tolerance are different, the more serious the destruction degree, the more the exudate in the cells; the destruction degrees of leaf cell membranes are judged on the basis of the relative conductivity of the isolated leaves in saline solution, thus judging genetypes of poplar with different salinity tolerance. The relative conductivity of the isolated leaves which are processed a certain time in high saline solution can rapidly and precisely reflect the salinity tolerance of the genetypes. By using the appraising technology, the salinity tolerance potential of the genetypes can be precisely judged by processing the leaves under certain salt concentration for 6-12min, thus saving labor and time, and having extremely high working efficiency and very wide application prospect.

Description

technical field [0001] The invention belongs to the plant detection of biotechnology, in particular to a method for identifying the salt-tolerant potential of poplar by utilizing the stress resistance ability of detached leaves. Background technique [0002] The saline-alkali soil is widely distributed in the world, and it accompanies the process of desertification, which not only causes the destruction of resources and the huge loss of agricultural production, but also poses a threat to the biosphere and ecological environment. With the growth of the world's population and the improvement of people's living standards, land and fresh water resources are becoming more and more scarce, and land salinization has become one of the increasingly serious environmental problems. The development and utilization of vast medium-to-severe saline-alkali land can not only prevent further aggravation of soil salinization, but also expand the planting area of ​​farmland, and solve the contr...

AI Technical Summary

Problems solved by technology

However, the growth period of poplar is long, and it takes a long time to evaluate the growth shape, phenological traits, growth period, etc., which is time-consuming and labor-intensive. The cost of molecular identification is high, which consumes manpower and material resources. Impact

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