Application of ascorbic acid to improvement of plant photosynthesis efficiency

A technology of ascorbic acid and photosynthetic efficiency, applied in the fields of application, plant growth regulator, plant growth regulator, etc., can solve the problem of reducing crop leaf transpiration rate, stomatal conductance concentration net photosynthetic rate, affecting soybean water and nutrient absorption, affecting Plant growth, crop yield and other issues, to achieve the effect of increasing net photosynthetic rate, increasing growth and yield, and reducing H2O2 accumulation

Inactive Publication Date: 2015-09-09
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aluminum stress mainly causes toxicity to the soybean root system, affects the photosynthetic efficiency of soybean, has a certain influence on the regulation of leaf stomata, increases the stomatal resistance, causes the gas exchange inside and outside the leaf t

Method used

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  • Application of ascorbic acid to improvement of plant photosynthesis efficiency
  • Application of ascorbic acid to improvement of plant photosynthesis efficiency
  • Application of ascorbic acid to improvement of plant photosynthesis efficiency

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: The cultivation and processing of SB, RB plants, concrete steps are as follows:

[0020] 1. The experimental materials are SB and RB seedlings

[0021] After selecting plump SB and RB seeds to sterilize and accelerate germination, accelerate germination in the dark at 25°C, and transfer to 0.5mmol / L CaCl when the root grows 2cm 2 Equilibrate in the solution for one day, then transfer to 1 / 4 Hoagland's culture medium for one week, transfer to Hoagland's culture medium after growing two leaves, and use it for this experiment when the seedlings grow to four pairs of leaves.

[0022] 2. Prepare AsA treatment solutions with different concentrations (0.1, 0.5, 2, 5, 10 mmol / L) and AlCl with different concentrations (0, 50, 100, 200, 400 μmol / L) 3 treatment fluid.

[0023] 3. Treat SB and RB seedlings with the above-mentioned AsA gradient concentration for 24 hours, and take samples for H 2 o 2 Content, stomatal opening, cytoplasmic membrane H ＋ - Determina...

Embodiment 2

[0025] Example 2: Adopt the SB, RB blade after the 3rd step process in embodiment 1 to carry out H 2 o 2 content, leaf stomatal opening and plasma membrane H ＋ - Pump activity assay

[0026] 1.H 2 o 2 Content determination: xylenol orange method. Weigh fresh plant leaves, add according to the ratio of 1g of material to 1mL of pre-cooled acetone extractant, add a little quartz sand to grind into a homogenate, transfer to a centrifuge tube and centrifuge at 12000g at 4°C for 20min, discard the residue, and the supernatant is Sample extract; respectively with ddH 2 O Prepare reagent A (containing 3.3 mmol / L FeSO 4 , 3.3 mmol / L (NH 4 ) 2 SO 4 , 412.5 mmol / L H 2 SO4 ) and reagent B (containing 165 μmol / L xylenol orange, 165 mmol / L sorbitol), before use, reagent A and reagent B are mixed in a ratio of 1:10 (v / v) to form a working reagent; this working reagent with H 2 o 2 The solution to be tested was mixed in a ratio of 2:1 (v / v), developed in a water bath at 30°C f...

Embodiment 3

[0035] Example 3: According to the above SB, RB leaf cell H 2 o 2 Content, stomatal opening, cytoplasmic membrane H ＋ - As a result of the determination of the pump activity content, 2 mmol / L was selected as the optimal treatment concentration of AsA. In order to verify that under the stress of different aluminum concentrations (0, 100 μmol / L), 2 mmol / L AsA has a greater promotion effect on the photosynthetic characteristic parameters of different varieties of soybeans, the optimal concentration of 2 mmol / L AsA treatment solution was used for the examples 1 For the treatment in the third step, the SB and RB seedlings were respectively treated with AsA concentration of 0 as a control, and the plasma membrane H of the plant leaves was measured for 24 hours ＋ - pump activity ( image 3 A, 3B).

[0036] from image 3 It can be seen that after treatment with 2 mmol / L AsA, the H of soybean leaves ＋ -Pump increased in different degrees compared with soybean plants under the s...

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Abstract

The present invention discloses novel application of ascorbic acid to improvement of plant photosynthesis efficiency. The invention is as below: using ascorbic acid solution with concentration gradient of 0.1,0.5,2,5,10 mmol / L to treat different varieties of plants for 24 hours, and respectively measuring physiological and biochemical parameters of leaves; stressing by using AlCl3 with different aluminum concentration gradients and different aluminum concentration gradients containing 2 mmol / L-ascorbic acid for 24 h, and measuring the photosynthetic characteristic parameters and physiological and biochemical indices of leaves respectively; and comparing the experimental results with the control plants. After ascorbic acid administration, H2O2 accumulation in aluminum sensitive soybean and Al tolerant soybean leaf cells decrease, and H + - pump activity and stomatal aperture and photosynthetic parameters are restored and improved; and these changes help promote plant stomatal opening and enhance photosynthesis and respiration of soybean, thereby enhancing the resistance to aluminum stress of plants.

Description

technical field [0001] The invention belongs to the field of plant photosynthesis and relates to a new application of ascorbic acid, in particular to the application of ascorbic acid in improving plant photosynthesis efficiency. Background technique [0002] Photosynthesis is the process by which plants use light energy to convert carbon dioxide and water into organic carbohydrates and store energy. As we all know, the essence of crop production is that crops use light energy to convert CO in the air 2 Translated into an organic matter storage process, dry matter accumulation depends on carbon assimilation. It is generally believed that 90% to 95% of the biological yield of crops comes from the products of photosynthesis, and only 5 to 10% of the substances come from the mineral nutrients absorbed by the roots. Stomatal conductance in photosynthetic parameters is a measure of the smoothness of water and gas exchange between plants and the atmosphere. Stomata are important ...

Claims

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Application Information

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IPC IPC(8): A01N43/08A01P21/00
Inventor 李昆志周小华陈东杰谷照虎陈丽梅
Owner KUNMING UNIV OF SCI & TECH
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