Application of Nifeviroc in preparing antitumor drug

An anti-tumor drug and tumor technology, which is applied in the field of medicine, can solve the problems of poor tumor treatment effect and many toxic reactions, achieve the reduction of directional migration ability and non-directional migration ability and in vitro invasion ability, significant effect, inhibition of invasion and transfer effect

Inactive Publication Date: 2018-08-10
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide the application of nifeviro in the preparation of antitumor drugs, to solve the problems of poor tumor treatment effect and more toxic reactions of existing drugs

Method used

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  • Application of Nifeviroc in preparing antitumor drug
  • Application of Nifeviroc in preparing antitumor drug
  • Application of Nifeviroc in preparing antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1C

[0030] Embodiment 1CCK-8 method detects cell proliferation

[0031] Cells in good growth state (non-small cell lung cancer A549 cells, non-small cell lung cancer NCI-H520 cells, human bronchial epithelial HBE cells, liver cancer HepG2 cells and breast cancer MCF-7 cells) were taken, digested with 0.25% trypsin, and mirrored. After observing the retraction of the cells, the digestion was terminated, centrifuged at 1000rpm for 5min, and the cells were collected. After staining with trypan blue, the cells were counted by a cell counter, and the cell density was adjusted to 4×10 4 cells / ml, seeded in 96-well plate, 100 μl per well (3×10 3 cells), set CO 2 cultured in a constant temperature incubator. The control group and the administration group were set up (the concentrations of Nifeviroc were 1.875, 3.75, 7.5, 15, and 30 μM respectively), and 5 multiple wells were set in each group. After the cells adhered to the wall, the original culture solution was discarded, and the admi...

Embodiment 2

[0034] Example 2 Annexin V-FITC / PI double staining method to detect cell apoptosis

[0035] Cells in good growth state (non-small cell lung cancer A549 cells, non-small cell lung cancer NCI-H520 cells, human bronchial epithelial HBE cells, liver cancer HepG2 cells and breast cancer MCF-7 cells) were digested with 0.25% trypsin to prepare Single cell suspension at 1 x 10 5 Inoculate in a 6-well culture plate at a density of 1 / well and store at 37°C, 5% CO 2 Incubator cultivation. After the cells adhered to the wall, the original culture medium was discarded, and the same volume of different concentrations of Nifeviroc (0, 1.875, 3.75, 7.5, 15, 30 μM) was added for intervention in groups, with 3 replicate wells for each group. After being treated with different concentrations of Nifeviroc for 48 hours, the cells were digested with EDTA-free trypsin and collected by centrifugation; the cells were washed three times with pre-cooled PBS buffer at 4°C, centrifuged at 1000 rpm for ...

Embodiment 3

[0039] Example 3 Cell clone formation experiment

[0040] Non-small cell lung cancer A549 cells in good growth state were digested with 0.25% trypsin to prepare a single cell suspension, seeded in a 24-well culture plate at a density of 100 cells / well, and placed at 37°C and 5% CO 2 Incubator cultivation. After the cells adhered to the wall, the original culture medium was discarded, and the same volume of different concentrations of Nifeviroc (0, 1.875, 3.75, 7.5, 15, 30 μM) was added for intervention in groups, with 3 replicate wells for each group. After 48 hours of Nifeviroc at different concentrations, change the fresh medium to continue culturing for 2-3 weeks, and change the medium every 72 hours; when clones visible to the naked eye appear in the culture plate, terminate the culture, discard the cell culture medium, and carefully soak in PBS. Wash twice, fix at room temperature for 15 minutes; remove the fixative, and stain with crystal violet for 30 minutes; slowly w...

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Abstract

The invention provides application of Nifeviroc in preparing an antitumor drug, and provides an antitumor drug containing Nifeviroc. As is proved by cell experiments, after Nifeviroc acts on tumor cells, the cell cycle progress can be interfered, and the cell growth is inhibited, so that the cell cycle is stagnant at the G0-G1 stage; meanwhile, cell apoptosis can also be induced by increasing theactive oxygen content in the cells and lowering the mitochondrial membrane potential; meanwhile, Nifeviroc has an obvious effect of inhibiting invasion and metastasis of the tumor cells, by inhibitingadhesive power of the tumor cells to an extracellular matrix, the directed migration capability, the non-targeted migration capability and the in-vitro invasion capability of the cells are lowered, and the increase in size of the tumors in vivo can also be inhibited. As can be seen, Nifeviroc has a good effect of resisting the activity of the tumor, can be used for preparing the antitumor drug, does not have an obvious side effect, and has a good application prospect.

Description

technical field [0001] The invention relates to the application of nifeviro, in particular to the application of nifeviro in the preparation of antitumor drugs, and belongs to the technical field of medicine. Background technique [0002] Malignant tumor is a major chronic disease that seriously threatens human health, and is one of the most serious public health problems in my country and even in the world. Tumor control has become the health strategy focus of governments around the world. According to the global report issued by the International Agency for Research on Cancer (IARC) of the World Health Organization (WHO), the incidence and mortality of cancer in my country are at a moderate level, ranking 72nd and 30th among 184 countries and regions in the world. Lung cancer, liver cancer, breast cancer, gastric cancer and colon cancer are the main common malignant tumors. For early and mid-stage patients, surgery is the preferred treatment option. However, due to the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/454A61P35/00
CPCA61K31/454A61P35/00
Inventor 林治华王娟舒茂王远强王锐胡勇陈诚
Owner CHONGQING UNIV OF TECH
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