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Method for preparing amnesic shellfish poison domoic acid through marine diatom symbiotic bacteria fermentation

A domoic acid and bacterial fermentation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of long domoic acid cultivation cycle, short fermentation time, low cost, etc., and achieve high market prospects With the effect of economic value, simple steps and low cost

Active Publication Date: 2019-01-22
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology uses sea-dwelling algae (Crypthecodiniphores) called Ponsatoa Spiricanus LZ-7 with very fast cell growth rates that produce large amounts of dodecacetone succinate from lactic acids produced during their metabolism process. These properties make this organisms ideal tools for studying biological processes such as photosynthesis or respiration. They also help researchers study how these compounds act on other living things like plants.

Problems solved by technology

Technological Problem: Dopamine (DA)) plays various roles such as promoting learning or improving cognitive function during fishings activities due its ability to stimulate certain areas within our body called nerves involved in senses like vision. However, it may cause damage if ingested too much for some people who use them regularly over time.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for preparing amnestic shellfish poison domoic acid by fermenting marine diatom symbiotic bacteria, comprising the following steps:

[0026] 1) Bacterial strain activation: inoculate Ponticoccus sp.LZ-7 kinds of Ponticoccus sp.LZ-7 species into the shake flask seed liquid medium, wherein the shake flask seed liquid medium includes the following components in mass percentage: yeast extract 4%, 3.5% peptone, 0.8% glucose, and natural seawater as the balance; culture at 31°C for 13 hours to obtain seed liquid of the activated strain;

[0027] 2) Seed liquid preparation: heat the seed culture liquid with steam, sterilize at 122°C for 30 minutes, and cool to 30.5°C. The seed culture liquid includes the following components in mass percentage: 4.5% yeast extract, 3% peptone, and 0.8% glucose , the balance is natural seawater; adopt the tinder inoculation method to inoculate the seed liquid of the activated bacterial strain into the seed culture liquid, the inoculum a...

Embodiment 2

[0032] A method for preparing amnestic shellfish poison domoic acid by fermenting marine diatom symbiotic bacteria, comprising the following steps:

[0033] 1) Bacterial strain activation: inoculate Ponticoccus sp.LZ-7 kinds of Ponticoccus sp.LZ-7 species into the shake flask seed liquid medium, wherein the shake flask seed liquid medium includes the following components in mass percentage: yeast extract 4%, 3% peptone, 0.6% glucose, and natural seawater as the balance; culture at 30.5°C for 13 hours to obtain seed liquid of the activated strain;

[0034] 2) Seed liquid preparation: heat the seed culture liquid with steam, sterilize at 121.5°C for 26 minutes, and cool to 30.5°C. The seed culture liquid includes the following components in mass percentage: 4% yeast extract, 2.5% peptone, and 0.6% glucose , the balance is natural seawater; adopt the tinder inoculation method to inoculate the seed liquid of the activated bacterial strain in the seed culture liquid, the inoculum a...

Embodiment 3

[0039] A method for preparing amnestic shellfish poison domoic acid by fermenting marine diatom symbiotic bacteria, comprising the following steps:

[0040] 1) Bacterial strain activation: inoculate Ponticoccus sp.LZ-7 kinds of Ponticoccus sp.LZ-7 species into the shake flask seed liquid medium, wherein the shake flask seed liquid medium includes the following components in mass percentage: yeast extract 5%, 4.5% peptone, 0.8% glucose, and natural seawater as the balance; culture at 31.5°C for 14 hours to obtain the seed solution of the activated strain;

[0041] 2) Seed solution preparation: heat the seed culture solution with steam, sterilize at 122.5°C for 32 minutes, and cool to 31°C. The seed culture solution includes the following components in mass percentages: 5% yeast extract, 4.5% peptone, and 0.8% glucose , the balance is natural seawater; adopt the tinder inoculation method to inoculate the seed liquid of the activated bacterial strain into the seed culture liquid,...

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Abstract

The invention relates to the technical field of marine microorganism fermentation, and discloses a method for preparing amnesic shellfish poison domoic acid through marine diatom symbiotic bacteria fermentation. The method solves the problems of long cultivation period and high cost of a method for preparing domoic acid through diatom cultivation and extraction. The preparing method comprises thesteps that strain activation is carried out, then a strain is inoculated to a seed culture solution for cultivation, then the strain is inoculated to a fermentation solution for fermentation to obtainamnesic shellfish poison domoic acid fermentation liquor, and finally the strain is soaked, extracted and purified to obtain the amnesic shellfish poison domoic acid. The method for preparing the domoic acid through bacterial fermentation is short in fermentation time, low in cost and relatively simple in metabolic regulation.

Description

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Claims

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Application Information

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Owner ZHEJIANG OCEAN UNIV
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