Enzyme-linked immuno-detection reagent case for red-tide toxin memony-losing shellfish poison

Abstract

An enzyme-liked immunodetection reagent box can rapid sensitively detecting red tide toxins Amnesic Shellfish Poisoning (ASP) in environmental sample. It utilizes synthetic high polymer ratio antigen DA-KLH, through many times immunity BLAB / c mouse, cell fusion, screening, induced ascites and protein column purification to obtain monoclonal antibody of idiocratic anti domoic acid (main constituent of Amnesic Shellfish Poisoning), selecting 1 : 1000 coat proportion and 1 : 8000 antibody dilution, developing out indirection competition enzyme-liked immunity analysing Amnesic Shellfish Poisoning (ASP) reagent box with sensitivity to 10 ng / ml.

Description

Technical field [0001] The invention relates to a rapid and convenient detection kit for analyzing and detecting the content of amnesic shellfish poison in environmental samples by combining the monoclonal antibody against amnesic shellfish poison with the amnesic shellfish poison antigen. Background technique [0002] At present, the analysis methods of shellfish poisoning with amnesia include biological mouse method, high performance liquid chromatography and immunochemical method. The biological mouse method is to inject the extract of biological tissues such as algae or shellfish into the abdominal cavity of the mouse. According to the death time of the mouse, compare the standard table to check the content of the amnesiac shellfish poison. This method has large errors and poor reproducibility. , Time-consuming and troublesome; high-performance liquid chromatography is to separate the extract of algae or shellfish and other biological tissues through liquid chromatograph...

AI Technical Summary

Problems solved by technology

The biological mouse method is to inject the extract of biological tissues such as algae or shellfish into the abdominal cavity of the mouse. According to the death time of the mouse, compare the standard table to check the content of amnesia shellfish poison. This method has large errors and poor reproducibility. , Time-consuming and troublesome; high-performance liquid chromatography is to separate the extract of algae or shellfish and other biological tissues through liquid chromatography after pretreatment, combine with ultraviolet or mass spectrometer detectors, and compare with the value of the standard to calculate the loss The content of memory shellfish poisoning, this method is accurate and mature, and can analyze and identify different isomers, but the disadvantage is that the pretreatment is troublesome, the instrument is expensive, and specialized analytical technicians are needed, and it is not suitable for the analysis of on-site and large-scale samples; The immunochemical method is to prepare specific antibodies (polyclonal antibodies or monoclonal antibodies) by immunizing animals, and use the binding reaction of antibodies to shellfish poisoning with amnesia, and make a detection kit according to the enzyme-linked immunoassay method, which is used for amnesia Analysis and detection of shellfish poisoning, this method is simple and fast, and can analyze a large number of samples at the same time, there are finished kits for sale abroad, but the price is expensive