Immune colloidal gold test paper strip for fast detecting diarrhetic shellfish poison and method for making the same

Abstract

The invention relates to the detection device of detecting the red tide toxin. An immune colloidal gold test paper to detect the lienteric toxin includes the sample pad, colloidal gold pad, NC film, the suction pad and PVC backing. The PVC backing is adhibited with the sample pad, colloidal gold pad, NC film and the suction pad. The colloidal gold pad is covered with the monoclonal antibody against okadaic acid marked by colloidal gold. The NC membrane is encrusted with the detection line composed by the okadaic acid and ovalbumin and quality controlling line composed by the multi antibody of sheep against rat. The test paper has high specialty, detection sensitivity, quick detection and simple pretreatment character. It needs not any device and convenience to carry and has low detection cost; the test paper has good stability and can be stored for six months in room temperature; the detection result has high veracity and precision.

Description

Technical field: [0001] The invention relates to a detection device for detecting red tide toxin, in particular to a rapid detection test strip for Diarrhetic shellfish poisoning (DSP), and also to a preparation method thereof. Background technique: [0002] The red tide disaster is becoming more and more serious and has become a global marine ecological disaster. Diarrheal shellfish poisoning is a class of fat-soluble polycyclic ether natural compounds produced by toxic red tide algae Pynoflagellates and Prorocentrum. The main components are Okadaic acid (OA) and its derivatives. The food chain transmission of shellfish and other rate-eating animals can cause poisoning to human eaters, resulting in diarrhea, nausea, vomiting and gastrointestinal colic, etc. OA is a strong protein phosphatase inhibitor and a strong cancer-promoting agent. It is a marine biotoxin of great concern at present. The analysis methods of diarrheal shellfish poison mainly include biological mouse ...

AI Technical Summary

Problems solved by technology

Among them, the mouse method is simple and easy, and is suitable for the detection of large-scale and batch samples. The disadvantage of this method is that it cannot distinguish the type and structure of the toxin, has large interference, and is not accurate; it is now widely accepted and relatively complete HPLC analysis The method is used in many countries, and can accurately analyze and determine the content and type of toxins, and the detection limit can be as low as ng / g. The popularization and promotion of this kind of analytical method makes it impossible to realize on-site detection

The immunochemical method is an analytical method established by using the specific binding of antibodies to antigens. It has the advantages of high sensitivity, strong specificity, simple and easy operation of equipment, and relatively simple sample pretreatment. It is suitable for on-site monitoring and a large number of samples under certain conditions. Rapid screening; at present, the ELISA detection kits for DSP produced by companies such as Germany and Japan are based on the principle of competitive enzyme-linked immunological reaction. The whole detection process takes 2-4 hours, and multiple samples can be detected at one time, which can be qualitative It can also be quantified, but there is a problem of false positives; because ELISA is a very sensitive technique, the variability of the analysis results is large, and the results of different analysts are different, and the result judgment often requires experienced operators, which is prone to false detection At the same time, there are many chemical structures of diarrheal shellfish poisoning components, and there are certain antigenic differences between isomers, and the detection time is relatively long, so it is difficult to realize real field operation

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