The invention discloses a one-step
seedling culture method of wheat anther, which comprises the following steps of: selecting young spike of wheat with wheat
pollen cells developing to mononuclear
metaphase to
late phase, and pretreating for 2 days at low temperature (2-4 DEG C); after conventional disinfection, stripping anther tissue from the pretreated young spike of wheat, and inoculating the anther tissue on one-step
seedling culture medium for shade culture; and when growing visible
callus, placing the cultured anther tissue under 1500Lx lighting conditions and culturing for a week, and then increasing the
light intensity to 3000Lx-4000Lx and continuously culturing for two weeks to produce
pollen plants with vigorous roots and seedlings. The method omits two necessary technical operation links of induction of adventitious buds and induction of adventitious roots by the
pollen callus, the culture cost is reduced by nearly 50%, the culture days are shortened by 40-50 days, the average induction rate of green seedlings is equivalent to that of multistep
seedling, the albino seedling rate is generally reduced by 10 percent, and test-tube seedlings are vigorous and has developed
root system, connecting parts of rootstalks have little or no
callus tissue residues, and the
transplantation survival rate of the test-tube seedlings is high.