30 results about "Tissue residue" patented technology

A contrast-enhanced ultrasound imaging method and a method for regional detection and development of a contrasted image. The method for regional detection of a contrasted image comprises: acquiring a tissue signal and a contrast signal in a contrast-enhanced ultrasound imaging process (S201); preprocessing respectively the tissue signal and the contrast signal (S202); comparison processing the preprocessed tissue signal and the contrast signal (S203); by means of a threshold segmentation approach, segmenting an image formed when comparison processed into a contrast agent effect region, a tissue residue effect region, and a noise effect region (S204); and, respectively marking corresponding positions on the contrasted image as a contrast agent region, the tissue residue effect region, and the noise effect region (S205). The regional detection method greatly increases the CTR of a contrast-enhanced ultrasound image, facilitates clinical observation, and enhances the contrast agent signal, thus allowing for a reduced contrast agent dosage injected during contrast-enhanced ultrasound imaging and effectively reduced examination costs.

The invention discloses a one-step seedling culture method of wheat anther, which comprises the following steps of: selecting young spike of wheat with wheat pollen cells developing to mononuclear metaphase to late phase, and pretreating for 2 days at low temperature (2-4 DEG C); after conventional disinfection, stripping anther tissue from the pretreated young spike of wheat, and inoculating the anther tissue on one-step seedling culture medium for shade culture; and when growing visible callus, placing the cultured anther tissue under 1500Lx lighting conditions and culturing for a week, and then increasing the light intensity to 3000Lx-4000Lx and continuously culturing for two weeks to produce pollen plants with vigorous roots and seedlings. The method omits two necessary technical operation links of induction of adventitious buds and induction of adventitious roots by the pollen callus, the culture cost is reduced by nearly 50%, the culture days are shortened by 40-50 days, the average induction rate of green seedlings is equivalent to that of multistep seedling, the albino seedling rate is generally reduced by 10 percent, and test-tube seedlings are vigorous and has developed root system, connecting parts of rootstalks have little or no callus tissue residues, and the transplantation survival rate of the test-tube seedlings is high.

The invention provides a method for efficiently extracting sheep viscus tissue protein, i.e. a frozen crushing homogenate method and a vortex mixing method are adopted, the protein quantification is carried out on the extracted viscus tissue protein of livers, spleens, lungs, kidneys and the like of the sheep, the extracted protein is separated through polyacrylamide gel electrophoresis, and the expression of the transgenosis sheep green fluorescent protein (GFP) genes is detected by western blot. The method has the technical characteristics that: 1, the concentration of the extracted protein is high, and the cost is low; 2, the method is applicable to the extraction of viscus tissue protein of adult or young sheep; and 3, insoluble protein is little, the abandoned tissue residue is little, and important practical values are realized.

The invention discloses a method for coproduction of pistacia chinensis bunge essential oil and polyphenol. The method comprises the following steps of: naturally airing pistacia chinensis bunge leaves; extracting the pistacia chinensis bunge essential oil by adopting a supercritical CO2 fluid extraction method; crushing pistacia chinensis bunge tissue residues obtained by extracting the essential oil; and carrying out polyphenol extraction. According to the method disclosed by the invention, source forest fostering residues are used as raw materials to carry out the coproduction of the pistacia chinensis bunge essential oil and the polyphenol; in a process of obtaining the high-quality essential oil, the subsequent extraction of the polyphenol is carried; two substances with high value and good application prospect are dug from forestry wastes, so that the comprehensive utilization value of an important energy plant pistacia chinensis bunge is greatly improved; the method has the very important meanings in accelerating the development of a biological energy source industry and improving the economic and social benefits of energy source plants.

The invention relates to a method for increasing the yield of neural stem cells in adult nerve tissues by utilizing organotypic culture. The method is characterized in that the method comprises organotypic culture and cell culture, and all the operations are completed on an aseptic operating platform. The method has the advantages and positive effects that organotypic culture is adopted and then papain digestion and blowing and beating dispersion are carried out seven days later, so that the quantity of obtained living cells is higher than that of the living cells obtained through direct enzymatic digestion by more than three times; another participation factor is mass multiplication of the neural stem cells in the organotypic culture process, thus increasing the total of the stem cells; another technological improvement is that the cells are directly inoculated in a poly-L-ornithine (PLO) coated cell culture plate after tissue dispersion, and tissue residues (including cell debris) are removed several hours later by changing a culture solution, thus omitting the time-consuming and tedious step of gradient centrifugation and also avoiding the cytotoxicity caused by most gradient centrifugation solutions.

The invention relates to a bioactive tissue fluid separating and filtering device, which comprises a base, wherein the base is provided with a fluid bearing funnel; at least one separating and filtering assembly is arranged at the upper part of the fluid bearing funnel; the bottom of the fluid bearing funnel is connected to a storage tank through a pipe; the separating and filtering assembly comprises a separating and filtering drum; a stirring paddle, a filter net and a porous support plate are sequentially arranged in an inner cavity of the separating and filtering drum from top to bottom; and a transfer bar for driving the stirring paddle to rotate is arranged in the separating and filtering drum in a penetrating manner. The invention further relates to a working method of the bioactive tissue fluid separating and filtering device. The defects of existing single-stage filtering and multi-stage filtering are overcome; the filter net is not easily blocked, effective tissue is completely filtered, the tissue residues are few, the product quality is high, the operation is convenient and fast, and a filtered fluid is not in contact with air.

The present invention discloses a method for synchronously separating chloroplast and mitochondria of a high purity plant. The method comprises: A, adding leaf to a CIB buffer liquid, grinding, filtering sequentially with a 70 [mu]m filtration screen, a 35 [mu]m filtration screen and a 15 [mu]m filtration screen, and carrying out centrifugation on the filtrate for 5 min at 200*g to remove tissue residue and cell debris; B, carrying out centrifugation on the filtrate for 10 min at 1000*g, and collecting chloroplast-rich precipitate; C, collecting the supernatant after the centrifugation in the step B, filtering with a 5 [mu] filtration screen, carrying out centrifugation on the filtrate for 10 min at 2000*g, removing the precipitate, carrying out centrifugation on the supernatant for 10 min at 17000*g, and collecting mitochondria-rich precipitate; D, washing the precipitates respectively with a washing buffer liquid, and digesting with DNase I to remove background genome contamination; and E, carrying out centrifugation on the chloroplast precipitate for 10 min at 1000*g, removing the supernatant, carrying out centrifugation on the mitochondrial precipitation for 10 min at 17000*g, removing the supernatant, and washing the precipitates with a washing buffer liquid.

The invention discloses a chemotactic antibacterial nanometer material which comprises graphene oxides and polydopamine coating the surfaces of the graphene oxides, wherein antibacterial peptides areadsorbed on the surface of the polydopamine; and a chemotactic agent is loaded on the antibacterial peptides through a chemical reaction. The problems that bacteria invade organisms, and bacteria andmedicines are remained in tissues are solved. The invention also discloses a preparation method and application of the chemotactic antibacterial nanometer material.

The invention provides an ultrasound contrast imaging method and apparatus. The method comprises: S1. transmitting a plurality of pulse waveform sequences, and acquiring echo signals of various pulses; S2. processing echoes of the various pulses to extract non-linear components of the echoes, and acquiring amplitude information about the non-linear components; S3. selecting echoes of any one or more pulses for processing to extract linear components of the echoes, and acquiring amplitude information about the linear components; S4. using the amplitude information about the non-linear components and that of the linear components to generate a non-linear parameter; and S5. performing imaging processing on the non-linear parameter. In the method, by simultaneously using amplitude information about linear components and non-linear components of an echo to generate a non-linear parameter, and performing imaging after processing this parameter, tissue residues can be effectively inhibited, and the dynamic range of a contrast agent signal is increased, thereby improving the CTR and contrast resolution of a contrast image.

The embodiment of the invention discloses a method for cleaning an decellularization reagent SDS, and belongs to the technical field of decellularization reagent post-treatment. The invention discloses a method for cleaning a decellularization reagent SDS, wherein decellularized tissues obtained by performing decellularization treatment by using SDS are sequentially soaked in an ethanol solution and a potassium ion solution. According to the invention, SDS decellularized tissues are sequentially soaked in an ethanol solution and a potassium ion solution, so that residual SDS in the decellularized tissue can be effectively removed, the content of the residual SDS is controlled to be free of cytotoxicity limit, the treatment time is shortened to 2-4 h, the SDS cleaning and removing efficiency is greatly improved, safety guarantee is provided for decellularization treatment of the SDS for the tissue, and industrial development is promoted; and the method disclosed by the invention is short-time and efficient, does not introduce other toxic substances, is safe and reliable, and is suitable for cleaning most SDS with decellularized allogeneic and xenogeneic tissue residues.

A method for isolating sulphated glycosaminoglycans washes a mechanically cleaned tissue, exposes tissue in a solution of 0.1M phosphate pH 5.8-6.0 buffer heated to a temperature of 65° C. for 30 minutes, overcooks the tissue in activated 0.1-0.4% papain at 65° C. for 6-24 hours, cools the papain digest to 4° C., removes fats and undigested tissue residues, selectively isolates the sulphated glycosaminoglycans for 4-10 hours on a solid carrier, obtained from bone tissue collagen with particle sizes ranging from 0-01 to 0.35 cm3, washes the carrier with 0.05-0.1 N-hydrochloric acid, degreases and eluates the carrier with a solution of 0.6-0.15 N-mineral salt in 0.8 N-acetic acid or in 0-01-0-025 N-alkali liquor, precipitates the sulphated glycosaminoglycans with ethanol, centrifuges with 1500 r.p.m. for 15 minutes at a temperature of 4° C., washes the precipitate with ethanol or acetone, and dries and sterilizes the precipitate.

The invention provides a container special for operating room nursing isolation instruments, and relates to the technical field of medical instrument containing. The container special for the operating room nursing isolation instruments comprises a box body, a support is fixedly connected to the upper inner wall of the box body, a supporting plate is fixedly connected to the inner wall of the support, a drawer is slidably connected to the upper surface of the supporting plate, and the lower side wall of the drawer and the left and right side walls of the support are all fine-hole steel wire meshes; the inner wall of the upper portion of the box body is fixedly connected with an upper partition plate, and the left side face of the upper partition plate and the left inner wall of the upper portion of the box body are each provided with a first ultraviolet lamp and an excimer discharge lamp. The medical instruments placed in the drawer are sterilized and disinfected through the excimer discharge lamp and the ultraviolet lamp, air is pumped outwards through the exhaust fan, the space where the drawer is located can be kept dry, microwave pyrolysis equipment is used for conducting microwave carbonization treatment on blood stains, tissue residues and germs on the surfaces of disposable articles and metal medical instruments after a hand operation, and the medical instruments are placed in the drawer. Bacterial viruses can be prevented from being infected and attached everywhere.

The invention discloses a separation method of soluble boron in plants. The separation method comprises the following steps: (1) washing, drying, crushing and sieving plant samples; (2) weighing a certain amount of powder sample and putting the powder sample into a needle cylinder with a sieve plate and an end cap; adding water to immerse the sample and ultrasonically vibrating; (3) taking off the end cap of the needle cylinder of an injection syringe and putting the needle cylinder of the injection syringe into a screw-cap centrifugal pipe; centrifuging, separating and collecting filtrates; and (4) repeating the step (2) of adding the water and vibrating, and the step (3), totally separating for 5-10 times, and combining all the filtrates so as to completely separate the soluble boron in the plant samples. The invention establishes the separation method of the soluble boron in the plants; the method can be used for separating the soluble boron in plant sample fluid from non-soluble boron in tissue residues; the method has the characteristics of simplicity and reliability, and wide application prospect, and provides new technical supports for researches in the fields including plant boron nutrition, physiology and biochemistry of the boron, biogeochemical cycles of the boron and the like.

The invention relates to a side staple type stapler. It includes a hand-held part and a functional part; the hand-held part includes a handle and a glans cover that is arranged at the front end of the glans cover and can move back and forth; the functional part includes a side nail assembly, and the side nail assembly includes a cutting wheel shell group, a pushing block, a pushing structure and The ring is equipped with a number of nail magazines with nail channels; the cutting wheel shell group is rotatably connected to the front part of the handle, and the nail magazine is in the shape of a ring. The pushing structure is located under the staple bin and the pushing structure is connected to the cutting wheel shell group; staples and pushing blocks are arranged in the nail setting channel from inside to outside, and a pushing slope is provided on the inner cavity wall of the cutting wheel shell group. The advantages of the present invention are: 1. When the stapled position is close to the joint between the foreskin and the glans and the circumcision is close to the stapled position, the effect of cutting the foreskin is the best; 2. Cutting and anastomosis from the side of the tissue can be more effective. Good to avoid tissue residue, easier to confirm the anastomosis phenomenon, easier anastomosis.