The present invention relates to the field of molecular biology, and particularly to a method for rapidly extracting RNA from animal tissues. The method mainly comprises two steps such as rapid separation of cells from tissue and rapid extraction RNA from cells with a nucleic acid extraction reagent, wherein the step 1 comprises: cutting tissue into small blocks, weighing, adding PBS, uniformly grinding to obtain tissue homogenate, carrying out low-speed centrifugation to precipitate large tissue residue so as to make the single cell suspend in the supernatant, taking the supernatant with thesuspended cells, adding PBS, uniformly mixing, and carrying out centrifugation to remove the supernatant to obtain precipitate, ie., the separated cells, and the step 2 comprises: adding a nucleic acid extraction reagent to the cells, uniformly mixing, carrying out room temperature standing to lysing the cells, heating for 10 min at a temperature of 75 DEG C, and cooling the sample by placing on ice so as to obtain the prepared RNA for gene expression level detection or gene cloning and other works. According to the present invention, the method has advantages of simple operation, high speed,low reagent cost, no toxicity and no odor.