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A method for determining residual benzimidazoles in animal tissue

A technology of benzimidazole and animal tissue, applied in the field of determination of residual benzimidazole drugs in animal tissue, achieving good accuracy and simple and convenient purification operation

Inactive Publication Date: 2017-04-19
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defect that the existing high-performance liquid chromatography method needs to go through complex degreasing and solid phase extraction purification treatment for the determination of benzimidazole drug residues in animal tissues, and provides a method for determining the residues of benzimidazoles in animal tissues. The method of benzimidazole drugs, the sample pretreatment of the present invention is simpler

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  • A method for determining residual benzimidazoles in animal tissue
  • A method for determining residual benzimidazoles in animal tissue
  • A method for determining residual benzimidazoles in animal tissue

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[0026] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0027] 1. Measurement method

[0028] 1. Sample pretreatment process

[0029] Accurately weigh 5.0g of meat sample, add 20mL of ethyl acetate, 0.15mL of 50wt% sodium hydroxide solution and 1mL of 1wt% di-tert-butylphenol solution. After ultrasonic extraction for 10 minutes, centrifuge at a speed of 10000r / min for 5 minutes, use a disposable dropper to draw the supernatant into an eggplant-shaped bottle, extract twice, combine the extracts, and spin evaporate to dryness.

[0030] Dissolve the remaining residue after evaporation to dryness above with 1.5mL of acetonitrile, vortex for 5min, add 4.5mL of 1.5mol / L hydrochloric acid solution, vortex evenly, shake for 5mi...

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Abstract

The invention discloses a method determining residue benzimidazole medicine in an animal tissue, wherein the method comprises the following steps: (1) extracting an animal tissue sample with ethyl acetate, and evaporating an extracted solution; (2) dissolving the residue after evaporating in step (1), adjusting a solution to be acid, adding a purifier polyN-ethylene pyrrolidone-dDivinyl benzene sulfonic acid sodium for adsorption, separating, desorbing the purifier with alkali solution and violating the obtained adsorbed solution; (3) dissolving the residue after violating in step (2) with a mobile phase, then performing chromatographic determination by using a high performance liquid chromatograph. When determining the residue benzimidazole medicine in the animal tissue, the method has good accuracy, flexibility and reproducibility. Compared with the existing method, the polyN-ethylene pyrrolidone-dDivinyl benzene sulfonic acid sodium is adopted as the purifier of the benzimidazole medicine and the purifying operation is more simple and convenient.

Description

technical field [0001] The invention relates to a method for determining residual benzimidazole drugs in animal tissues. Background technique [0002] Parasitic diseases are the most common diseases in the breeding industry, which cause huge economic losses every year. Some of the parasites can be transmitted to humans and cause human infection. At present, benzimidazole anthelmintics are mainly residues in meat products and are harmful to humans. After people eat meat products with excessive residues of benzimidazoles, it will cause serious adverse effects, causing acute and chronic toxic effects on the human body directly or indirectly through the environment and food chain, causing parasite drug resistance In the long run, it will also affect the development of the breeding industry and the normal order of the market. The existing risk factors related to meat products mainly include the following: oxfendazole, fenbendazole, albendazole, mebendazole, flubendazole, thiabe...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 熊琳李维红郭志廷杨晓玲高雅琴
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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