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Method for cleaning decellularization reagent SDS

A decellularized and decellularized tissue technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of unfavorable acellular tissue repair materials industrialized production, time-consuming, high cytotoxicity, etc., to promote the development of the industry, The effect of improving the cleaning and removal efficiency

Inactive Publication Date: 2021-02-12
上海亚朋生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In summary, although SDS is a relatively effective decellularization reagent, its cytotoxicity is relatively high, and it often takes a lot of time to thoroughly clean it, which is not conducive to the industrial production of decellularized tissue repair materials.

Method used

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  • Method for cleaning decellularization reagent SDS
  • Method for cleaning decellularization reagent SDS
  • Method for cleaning decellularization reagent SDS

Examples

Experimental program
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Effect test

Embodiment 1

[0031]The allogeneic skin in this example is human skin tissue with the dermis retained, with a thickness of 0.2-1.5mm and a surface area of ​​10mm×10mm. It is shaken and immersed in a 0.5% SDS aqueous solution for 24 hours to ensure SDS It penetrates completely into the tissue, and then uses the method of the present invention to clean the residual SDS. The specific operations are as follows:

[0032]The acellular allogeneic skin obtained above was immersed in a 75% ethanol solution with a volume fraction, and the shaker was shaken for 1 hour, during which the liquid was changed every 20 minutes. Then take out the allogeneic skin, immerse it in a 0.5M KCl aqueous solution with a molar concentration of potassium ions, shake the shaker for 1 hour, and change the liquid every 20 minutes. Ensure that the SDS deposited on the surface of the allogeneic skin is completely washed away.

[0033]In order to illustrate the feasibility of the method of this embodiment, the following verification is ...

Embodiment 2

[0042]The allogeneic tendon in this example is the tendon tissue taken from a human donor. The dense ends at both ends of the tendon bundles have a diameter between 3-15 mm. They are shaken in a 0.5% SDS aqueous solution. After immersing for 24 hours, it is ensured that the SDS completely penetrates into the tissue, and then the method of the present invention is used to clean the residual SDS. The specific operations are as follows:

[0043]The decellularized allogeneic tendon obtained above was immersed in a 75% ethanol solution with a volume fraction, and the shaker was shaken for 2 hours, during which the liquid was changed once every 40 minutes. Then take out the allogeneic tendon, immerse it in a KCl aqueous solution with a potassium ion molar concentration of 0.5M, shake the shaker for 2 hours, and change the liquid every 40 minutes. Ensure that the SDS deposited on the surface of the allogeneic tendon is completely washed off.

[0044]In order to illustrate the feasibility of this...

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Abstract

The embodiment of the invention discloses a method for cleaning an decellularization reagent SDS, and belongs to the technical field of decellularization reagent post-treatment. The invention discloses a method for cleaning a decellularization reagent SDS, wherein decellularized tissues obtained by performing decellularization treatment by using SDS are sequentially soaked in an ethanol solution and a potassium ion solution. According to the invention, SDS decellularized tissues are sequentially soaked in an ethanol solution and a potassium ion solution, so that residual SDS in the decellularized tissue can be effectively removed, the content of the residual SDS is controlled to be free of cytotoxicity limit, the treatment time is shortened to 2-4 h, the SDS cleaning and removing efficiency is greatly improved, safety guarantee is provided for decellularization treatment of the SDS for the tissue, and industrial development is promoted; and the method disclosed by the invention is short-time and efficient, does not introduce other toxic substances, is safe and reliable, and is suitable for cleaning most SDS with decellularized allogeneic and xenogeneic tissue residues.

Description

Technical field[0001]The embodiment of the present invention relates to the technical field of post-treatment of decellularization reagents, in particular to a method for cleaning SDS of decellularization reagents.Background technique[0002]Sodium dodecyl sulfate (SDS) is an anionic surfactant, easily soluble in water. It has important application value in genetic engineering protein preparation and antigen synthesis and other biochemical experiments and immunoassays. At the same time, SDS is an effective The decellularization reagent is widely used in the decellularization of allogeneic and heterogeneous organs / tissues. Although the decellularization effect of SDS is remarkable, it is difficult to clean SDS from the tissues, and it is easy to cause residue. Because SDS can dissolve almost all proteins, it can destroy the non-covalent bonds of the protein, thereby denaturing the protein and losing its natural conformation and function, so its residue will have significant toxic side ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/362A61L27/3604A61L27/3608A61L27/3687A61L2430/40
Inventor 陈金发胡凯白玉龙陈维明骆井万李淼汪晶晶
Owner 上海亚朋生物技术有限公司
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