One-step seedling culture method of wheat anther

Abstract

The invention discloses a one-step seedling culture method of wheat anther, which comprises the following steps of: selecting young spike of wheat with wheat pollen cells developing to mononuclear metaphase to late phase, and pretreating for 2 days at low temperature (2-4 DEG C); after conventional disinfection, stripping anther tissue from the pretreated young spike of wheat, and inoculating the anther tissue on one-step seedling culture medium for shade culture; and when growing visible callus, placing the cultured anther tissue under 1500Lx lighting conditions and culturing for a week, and then increasing the light intensity to 3000Lx-4000Lx and continuously culturing for two weeks to produce pollen plants with vigorous roots and seedlings. The method omits two necessary technical operation links of induction of adventitious buds and induction of adventitious roots by the pollen callus, the culture cost is reduced by nearly 50%, the culture days are shortened by 40-50 days, the average induction rate of green seedlings is equivalent to that of multistep seedling, the albino seedling rate is generally reduced by 10 percent, and test-tube seedlings are vigorous and has developed root system, connecting parts of rootstalks have little or no callus tissue residues, and the transplantation survival rate of the test-tube seedlings is high.

Description

technical field [0001] The invention belongs to the wheat cell engineering breeding technology in food crops, and further relates to a one-step seedling cultivation method for wheat anthers. Background technique [0002] Wheat is one of the most important food crops for humans. The genetic improvement of wheat through various ways is the basis of high quality, high yield and stable yield of wheat. The wheat haploid breeding technology developed in the 1970s has become one of the important modern wheat biotechnological breeding methods. played an important role in. At present, wheat haploid breeding has been using multi-step seedling formation technology, that is, wheat anther tissues are cultured in vitro on dedifferentiation medium to induce dedifferentiation of pollen cells to produce callus, and then callus is induced on redifferentiation medium Differentiate into rootless seedlings, and the third step is to transfer the induced rootless seedlings to the rooting medium...

AI Technical Summary

Problems solved by technology

At present, wheat haploid breeding has been using multi-step seedling formation technology, that is, wheat anther tissues are cultured in vitro on dedifferentiation medium to induce dedifferentiation of pollen cells to produce callus, and then callus is induced on redifferentiation medium Differentiate into rootless seedlings, and the third step is to transfer the induced rootless seedlings to the rooting medium for strong seedlings to take root. This anther culture multi-step seedling technology has complex training procedures, long training periods, high costs, and albino seedlings. High rate and other problems limit the further promotion and application of this technology

In recent years, individual scholars have carried out studies on the direct emergence of wheat anther culture, but the results are far from satisfactory, and a complete one-step seedling growth technology for anther culture has not been established so far.

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