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Method for early detecting toxicity of paralytic shellfish poison

An early detection, paralysis technology, applied in the field of bioengineering, to achieve the effect of simple and convenient operation, protection of public health and marine ecological environment safety

Inactive Publication Date: 2008-06-11
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a method for early detection of the toxicity of paralytic shellfish poisoning, which solves the problems of on-site, fast, large-scale sample detection and early detection of the toxicity of paralytic shellfish poisoning, which is better than the currently commonly used method in China. Compared with the mouse method, the time is 2-10 times earlier, and the individual does not have any symptoms, which is suitable for rapid on-site detection and analysis of large batches of samples and early detection of toxicity

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  • Method for early detecting toxicity of paralytic shellfish poison
  • Method for early detecting toxicity of paralytic shellfish poison
  • Method for early detecting toxicity of paralytic shellfish poison

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Embodiment

[0023] 1 Preparation of sample detection solution

[0024] After collecting the shellfish samples, clean the attachments on the shell, separate the shellfish from the shell, pick out the digestive glands and other soft tissues, dissolve the slurry in a slurry machine, dissolve it in 0.1mol / L HCl, and adjust the pH value to 3 with NaOH -4. Heating in a water bath for 5 minutes, after cooling, take the supernatant and centrifuge, the centrifuged supernatant is the sample stock solution, and the stock solution is diluted with 0.9% normal saline to obtain the sample test solution.

[0025] 2 mice exposed

[0026] Select healthy ICR (Institute of Cancer Research, American Cancer Institute) strain adult mice, weighing 25g-30g, and inject the mice intraperitoneally with a body weight dose of 1 μg / kg and 10 μg / kg of the test solution. After 15 minutes of exposure, blood was drawn from the femoral artery to kill the mice. The brain and skull of the mice were quickly removed in an ice ...

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Abstract

The invention discloses a method for early detection on the toxicity of the Paralytic shellfish poisoning toxin in the technical field of the biological engineering, which is to make the check solution by the on-site shellfishes and make the abdominal cavity injection to the mouse with the check solution to test the content of the acetylcholine in the mouse brain and the change on activity of the endothelial nitric oxide synthase. The Paralytic shellfish poisoning toxin can be gained from the content of the acetylcholine and activity of the endothelial nitric oxide synthase, which can make an early detection on the toxicity of the Paralytic shellfish poisoning toxin. The invention realizes the on-site and fast test and analysis to large amount of samples and the early detection on the toxicity, which reduces the time by two to ten times compared with the common mice method currently used in China, avoiding any occurrence of the individual symptom and applicable to the on-site and fast test and analysis to large amount of samples and the early detection on the toxicity. The invention is simple and convenient to operate with only test on change of common biochemical parameters required, which is applicable to promotion and use by the on-site and grass-root test departments.

Description

technical field [0001] The invention relates to a detection method in the technical field of bioengineering, in particular to a method for early detection of the toxicity of paralytic shellfish poisoning. Background technique [0002] Frequent outbreaks of red tides not only worsen the marine environment, but more importantly, many algae in red tides can produce toxins, which are enriched in seafood and passed through the food chain to cause poisoning after eating seafood. Although algae toxins mostly come from toxic red tide algae, the toxins produced by algae are often enriched through shellfish, fish and other media, causing human poisoning. Therefore, these toxins are usually called shellfish poisons. Divided into the following five types: paralytic shellfish poisoning (PSP), diarrhea shellfish poisoning (DSP), amnesia shellfish poisoning (ASP), neuropathic shellfish poisoning (NSP) Shellfish poisoning is the most widely studied red tide toxin, and it is also the most h...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/78G01N21/31
Inventor 程金平张鉴达宋玉玲刘元嫄王文华仵彦卿
Owner SHANGHAI JIAO TONG UNIV
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