Compound for saxitoxin fluorescence detection and detection method
A technology for saxitoxin and fluorescence detection, which is applied in chemical instruments and methods, fluorescence/phosphorescence, chemical analysis by titration, etc., can solve the problems of high price and difficulty in obtaining toxin antibodies, etc., and achieves fast time and low cost. , the effect of huge application prospects
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[0056] Example 1: Preparation and identification of compound EuL1
[0057] The compound EuL1 was prepared as follows:
[0058]
[0059] Preparation and identification data of compound EuL1:
[0060] Add 1 equivalent of compound 1, 1.5 equivalent of compound 2, and 0.1 equivalent of Pd(PPh 3 ) 2 Cl 2 And 0.03 equivalent of CuI, add THF to dissolve it, and react at 45°C. After the reaction, it was quenched by adding water, and extracted with ethyl acetate. The organic phase obtained from the extraction was dried with anhydrous sodium sulfate and the solvent was removed under reduced pressure. The residue was separated and purified by silica gel column chromatography to obtain compound 3. The identification results using proton nuclear magnetic resonance spectrum and carbon nuclear magnetic resonance spectrum are as follows: 1 H NMR(CDCl 3 ,500MHz):δ8.55(br,1H),7.50(d,J=8.7Hz,2H),7.44(br,1H),7.39(br,1H),6.91(d,J=8.8Hz,2H) ,4.83(br,1H),3.97(t,J=6.6Hz,2H),1.06(t,J=7.4Hz,3H); 13 C NMR(C...
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[0066] Example 2
[0067] Fluorescence titration of probe EuL1:
[0068] Prepare the detection solution: Dissolve the probe compound EuL1 in double-distilled water, the concentration of the probe EuL1 aqueous solution is 50μM;
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