Detection device and method adopting lased-induced liquid fluorescence

Abstract

The invention discloses a detection device adopting lased-induced liquid fluorescence and relates to the technical field of fluorescence spectra. The detection device comprises an automatic injected sample cleaning system, a fluorescence excitation system and a beam splitting system, wherein the automatic injected sample cleaning system is located in the middle of the whole device, and the fluorescence excitation system and the beam splitting system are located on two sides of the automatic injected sample cleaning system respectively. A liquid-core optical fiber is filled with a to-be-measured liquid, fluorescence produced by excitation is transmitted in the liquid-core optical fiber along with incident light and accumulated and intensified continuously, a laser is all limited in the whole liquid-core optical fiber to be transmitted after coupled to enter the liquid-core optical fiber, exciting light interacts with the to-be-detected liquid continuously, the total effective exciting light path is extended, and the intensity of the fluorescence produced by excitation is accumulated continuously. The liquid-core optical fiber can be very long, so that the intensity of the fluorescence can be increased substantially in the optical fiber, and fluorescence detection of low-concentration substances can be realized.

Description

technical field [0001] The invention relates to the technical field of fluorescence spectroscopy, in particular to a high-sensitivity liquid fluorescence detection device. Background technique [0002] Fluorescence is a photoluminescence phenomenon. When a substance is irradiated by incident light of a specific wavelength (usually visible light, ultraviolet or X-ray), it absorbs light energy and enters an excited state, and immediately exits the excited state to emit light of a wavelength shorter than that of the incident light. Longer outgoing light. Once the incident light stops, the luminous phenomenon will disappear immediately. Emerging light with this property is called fluorescence. [0003] A fluorescence spectrometer consists of three basic components: a light source, a liquid sample cell, and a detector. The light source can be a single-wavelength light source, or a monochromator with continuously adjustable wavelength; Photomultiplier tube or CCD. [0004] Whe...

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Problems solved by technology

Patent 201110343868.8, titled "A liquid-core waveguide fluorescence detection device", utilizes a large-area excitation light source to irradiate the entire length of the liquid-core optical fiber, and all the liquid in the optical fiber can be excited by the laser to produce fluorescence, but a surface light source is used as the excitation light source When the excitation light energy cannot be concentrated, the excitation light intensity is weak, and it is difficult to generate strong fluorescence, which is also not conducive to the fluorescence detection of low-concentration solutions.

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