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Ordered SiO2 mesoporous assembled Cds, ZnS micro-array biochip preparing method

A biochip and microarray technology, applied in biological testing, chemical instruments and methods, measuring devices, etc., can solve problems such as photolysis, poor photostability, bleaching, etc., and achieve multi-efficiency, strong stability, and uniform distribution Effect

Inactive Publication Date: 2006-03-08
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, organic fluorescent dye labeling is a commonly used method because it has the advantages of high luminous intensity and good monitoring sensitivity. However, the fluorescence peak of organic fluorescent dyes is relatively wide, and the resolution in multi-component detection is not high, and the luminous wavelengths are different. It is required to have different excitation wavelengths, and its photostability is poor, and a long time will cause it to bleach or photodecompose
Although, in order to overcome these defects of organic fluorescent dyes, people have adopted inorganic compound semiconductor quantum dot materials as fluorescent labels, such as: Chinese patent (application number: 02139152.1) and using II-VI semiconductor quantum dots or nanoparticles such as CdS, ZnS as the core Then wrap the SiO 2 Layer, and then deposit an island-shaped gold layer and then graft it to biomolecules for detection, such as Chinese patent (application number 02109898.2), but there are detection limitations, and it cannot be a biochip similar to a microarray for multiple biological molecular detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment one: concrete steps are as follows:

[0020] 1. Ordered mesoporous SiO 2 Membrane preparation: the area is 12×30mm 2 The glass slide was used as the substrate, washed with water, acetone, and ethanol, rinsed with deionized water, and dried under an infrared lamp.

[0021] 2. Dissolve 0.38 mole template cetyltrimethylammonium bromide in a solvent mixed with 100ml ethanol and 30ml water under constant stirring, and prepare 1×10 -2 After stirring the F127 solution with a molar concentration for 5 hours, add 100 ml of a 0.2 molar concentration of tetraethyl orthosilicate solution and 100 ml of a 0.2 molar concentration of acetylacetone solution dropwise, and continue stirring for 3 hours; )5 days. Then, immerse the dried glass piece in the above-mentioned mixed solution, and pull it slowly at a speed of 26 cm / min. After taking it out and drying it under an infrared lamp, it will be coated with ordered SiO 2 Glass slides of mesoporous membranes were kept in a...

Embodiment 2

[0026] Embodiment two: this embodiment is basically the same as the above-mentioned embodiment, the difference is:

[0027] 1. With an area of ​​8×20mm 2 The silicon wafer is used as the substrate,

[0028] 2. Put 2.5×10 -2 The molar template agent P127 was dissolved in a solvent mixed with 150ml ethanol and 50ml water under constant stirring, and was prepared as 5×10 -3 After stirring the solution of molar concentration for 5 hours, add dropwise 100ml of 0.05molar concentration tetraethyl silicate solution and 100ml of 0.05molar concentration triethanolamine solution respectively, and continue to stir for 3 hours; aging (or aging) after sealing the mixed solution 5 days. Then, immerse the dried glass piece in the above-mentioned mixed solution, and pull it slowly at a speed of 36 cm / min. After taking it out and drying it under an infrared lamp, it will be coated with ordered SiO 2 The glass slide of the mesoporous membrane is stored in a desiccator;

[0029] 3. The coup...

Embodiment 3

[0032] Embodiment three: this embodiment is basically the same as the above-mentioned embodiment, the difference is:

[0033] 1. With an area of ​​8×20mm 2 The silicon wafer is used as the substrate,

[0034] 2. Dissolve 0.18 moles of template agent P127 in a solvent mixed with 210ml of ethanol and 70ml of water under constant stirring, and prepare 5×10 -2 After stirring the solution of molar concentration for 5 hours, add dropwise 100ml of 0.5 molar concentration tetraethyl orthosilicate solution and 100ml of 0.5 molar concentration ethylene glycol amine amine solution respectively, and continue to stir for 3 hours; aging (or aging) 7 days. Then, immerse the dried glass piece in the above-mentioned mixed solution, and pull it slowly at a speed of 20 cm / min. After taking it out and drying it under an infrared lamp, it will be coated with ordered SiO 2 The glass slide of the mesoporous membrane is stored in a desiccator;

[0035] 3. The coupling agent used is 30ml of γ-oxy...

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Abstract

This invention refers to a method for preparing micro array biochip for fluorescent detection, made from SiO2 mesoporous assembling CdS and ZnS semiconductor nano crystal. Said method comprises preparing order mesoporous SiO2 film, connecting mesoporous assembling coupling agent, assembling of CdS or ZnS in mesoporous and activation of assembling substrate.

Description

technical field [0001] The invention relates to a method for preparing a microarray biochip for fluorescent detection of semiconductor nanocrystals, in particular to an ordered SiO 2 A method for preparing a microarray biochip with mesoporous assembled CdS and ZnS semiconductor nanocrystals for fluorescence detection. Background technique: [0002] As we all know, one of the important means and methods for clinical diagnosis and treatment is immunological detection, and the method it relies on is biological detection. A simple and sensitive method. [0003] Modern biomedicine has clearly understood that the most harmful disease to the human body is also one of the diseases with the highest mortality rate, that is, malignant tumors, and its early diagnosis is undoubtedly of great significance to human health. One of the means of early diagnosis is high sensitivity, high accuracy and high selectivity is immunological biological detection. At present, clinical biological imm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N21/00C09K11/00
Inventor 张建成聂波刘国勇沈悦戴宁
Owner SHANGHAI UNIV
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