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Preparation method and application of copper nanocluster self-assembly

A self-assembly and copper nanotechnology, applied in nanotechnology, nanotechnology, nano-optics, etc., can solve the problems of cumbersome processing and achieve the effects of strong fluorescence stability, low detection limit, and rapid preparation

Inactive Publication Date: 2018-04-13
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Histamine is one of the main biogenic amines in food. Fish food histamine poisoning incidents occur from time to time. At present, international and national health organizations are further expanding the types of food for histamine detection and reducing the standard of histamine content. The existing group Amine detection methods include thin-layer chromatography, gas chromatography, high-performance liquid chromatography, capillary electrophoresis, electrochemical sensing, enzyme-linked immunosorbent method, etc. The sample pretreatment process of these methods is relatively cumbersome, and all of them involve the operation of some professional instruments. Therefore, it is necessary to develop a real-time, rapid and sensitive detection method for histamine.

Method used

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  • Preparation method and application of copper nanocluster self-assembly
  • Preparation method and application of copper nanocluster self-assembly
  • Preparation method and application of copper nanocluster self-assembly

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Experimental program
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Effect test

Embodiment 1

[0034] Preparation steps of ribbon-shaped copper nanocluster self-assembly: Weigh 12 mg of copper nitrate and dissolve it in 5 mL of ethanol, add 0.15 mmol of 2,3,5,6-tetrafluorothiophenol, and stir at 25 °C for 1 h; then Centrifuge, remove the supernatant, and keep the precipitate; then the precipitate is centrifuged and washed three times with ethanol, and dried with nitrogen to obtain a light yellow powder, which emits orange fluorescence under the irradiation of a 365nm ultraviolet lamp (see figure 1 ).

Embodiment 2

[0036] Morphological characterization steps of copper nanocluster self-assembly: disperse copper nanocluster self-assembly powder in ethanol solution, take 2 μL and drop it on copper grid covered with ultra-thin carbon film, and make transmission electron microscope sample after natural drying , put the sample holder in the lens barrel of a JEM-1011 transmission electron microscope, and observe its morphology at a suitable magnification. Depend on figure 2 It can be seen that the copper nanoclusters self-assembled during the synthesis process and formed a ribbon structure after self-assembly.

Embodiment 3

[0038] The UV-visible absorption spectrum characterization steps of the copper nanocluster self-assembly: disperse the copper nanocluster self-assembly powder in the ethanol solution, and measure its UV-visible absorption spectrum with a UV-visible spectrophotometer, as shown in image 3 As shown, its ultraviolet-visible spectrum has four characteristic absorption peaks at 255nm, 283nm, 330nm and 435nm, wherein the absorption peaks at 255nm and 283nm correspond to the ligand 2,3,5,6-tetrafluorothiophenol (TFTP) Ultraviolet characteristic absorption, the characteristic absorption peaks at 330nm and 435nm are the characteristic absorption of 2,3,5,6-tetrafluorothiophenol-copper nanoclusters.

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Abstract

The invention discloses a preparation method of a copper nanocluster self-assembly and application of the copper nanocluster self-assembly as a fluorescent probe to detection of histamine. 2,3,5,6-tetrafluorothiophenol serves as a ligand and a reductant to synthesize the banded copper nanocluster self-assembly with orange fluorescence in one step. The preparation method is a novel method for preparing the copper nanocluster self-assembly and has the advantages of being simple to operate, quick in preparation and low in cost. The synthesized copper nanocluster self-assembly has the advantages of broad excitation spectrum, narrow emission spectrum, high fluorescence quantum yield, strong fluorescence stability and the like, and besides, can be used for detecting histamine.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a method for preparing a copper nano-cluster self-assembly and the application of the nano-cluster self-assembly in histamine detection. Background technique [0002] Metal nanoclusters are a class of nanomaterials whose size is between metal atoms and nanoparticles, and whose diameter is generally below 2nm. As a new type of fluorescent material, metal nanoclusters have been used in biomarkers, bioimaging, and construction of fluorescent sensors. However, there are still some shortcomings in metal nanoclusters, such as weak fluorescence intensity of products and fluorescence quantum yield. Low, poor stability, etc., greatly limited its application in analysis and detection. It is reported in the current literature that compared with monodisperse metal clusters, aggregated metal clusters or metal cluster assemblies have high fluorescence intensity and good stab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/58B82Y20/00B82Y40/00B22F9/24G01N21/64
CPCB22F9/24B82Y20/00B82Y40/00C09K11/58G01N21/643G01N2021/6432
Inventor 刘继锋韩爱玲刘冰王硕
Owner TIANJIN UNIV OF SCI & TECH
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