A kind of rhodamine 6g-based chromium ion detection fluorescent probe molecule, preparation method and application

A fluorescent probe, chromium ion technology, applied in the field of chromium ion detection, can solve the problems of complex sample preparation, high detection cost, long test process, etc., and achieve the effects of good cell permeability, short resolution time, and small side effects.

Active Publication Date: 2018-08-03
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, although the common test methods for chromium ions generally have the characteristics of high sensitivity, there are still widespread problems such as high detection cost, complicated sample preparation, and long test process.

Method used

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  • A kind of rhodamine 6g-based chromium ion detection fluorescent probe molecule, preparation method and application
  • A kind of rhodamine 6g-based chromium ion detection fluorescent probe molecule, preparation method and application
  • A kind of rhodamine 6g-based chromium ion detection fluorescent probe molecule, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Synthesis of fluorescent probe molecules for detection of chromium ions based on rhodamine 6G

[0039] For specific synthetic routes, see figure 1 .

[0040](1) Preparation of 2-formyl-8-hydroxyquinoline: 0.32 g of 2-methyl-8-hydroxyquinoline (2 mmol) was dissolved in 20 mL of dioxane. Heat to 60°C, then weigh 0.27g of SeO 2 It was added to the above solution, and then the temperature was raised to 80° C., and the reaction mixture was heated to reflux for 10 hours under the protection of nitrogen. After the reaction, the reaction liquid was cooled to room temperature, and the obtained precipitate was washed with 8 mL of dioxane and 8 mL of dichloromethane, filtered, and vacuum-dried. The obtained crude product was passed through a silica gel chromatography column and eluted with petroleum ether: ethyl acetate = 9:1 (v:v), and the eluate was concentrated to obtain 0.14 g of a light yellow compound. Yield 40.5%. 1 H-NMR (500MHz, CDCl 3 ):δ10.2(s,1H),8.31-...

Embodiment 2

[0045] Example 2: Selectivity of fluorescent probe molecules for chromium ion detection based on rhodamine 6G for ultraviolet detection of chromium ions

[0046] Using CH 3 CN (acetonitrile): Tris-HCl buffer (trishydroxymethylaminomethane) (0.01mol / L, pH=7.4)=9:1 (v:v) solution controls the experimental conditions.

[0047] Chromium ion detection fluorescent probe molecules based on rhodamine 6G 3 CN:Tris-HCl=9:1 (v:v) was dissolved in a solvent and fixed to a 100mL volumetric flask to prepare a solution with a fluorescent probe molecular concentration of 10 μmol / L.

[0048] Divide the sample vials into 12 groups, add 5mL of rhodamine 6G-based chromium ion detection fluorescent probe molecules with a concentration of 10 μmol / L to each sample vial 3 CN: Tris-HCl (0.01mol / L, pH=7.4) = 9:1 (v:v) solution, the first bottle of solution was used as a blank group, and 75 μL of K with a concentration of 0.1mol / L was added to the other 11 groups + , Na + ,Co 2+ , Ni 2+ ,Zn 2+ ,P...

Embodiment 3

[0051] Example 3: Selectivity of fluorescent probe molecules for chromium ion detection based on rhodamine 6G for chromium ion fluorescence detection

[0052] Using CH 3 CN (acetonitrile): Tris-HCl (0.01mol / L, pH=7.4) = 9:1 (v:v) solution controls the experimental conditions.

[0053] Chromium ion detection fluorescent probe molecules based on rhodamine 6G 3 CN:Tris-HCl=9:1 (v:v) was dissolved in a solvent and fixed to a 100mL volumetric flask to prepare a solution with a fluorescent probe molecular concentration of 10 μmol / L.

[0054] Divide the sample vials into 12 groups, add 5mL of rhodamine 6G-based chromium ion detection fluorescent probe molecules with a concentration of 10 μmol / L to each sample vial 3 CN: Tris-HCl (0.01mol / L, pH=7.4) = 9:1 (v:v) solution, the first bottle of solution is used as a blank group, and 75 μL of K with a concentration of 0.1mol / L were added to the other 11 groups + , Na + ,Co 2+ , Ni 2+ ,Cu 2+ ,Zn 2+ ,Pb 2+ ,Cd 2+ , Li + , Fe 3+ ,...

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Abstract

The present invention discloses a chromium ion detection fluorescence probe molecule based on Rhodamine 6G, a preparation method and uses thereof, wherein the fluorescence probe molecule has the following structure defined in the specification. The invention further discloses a preparation method of the chromium ion detection fluorescence probe molecule based on Rhodamine 6G, and uses of the chromium ion detection fluorescence probe molecule based on Rhodamine 6G in chromium ion detection.

Description

technical field [0001] The invention belongs to the field of organic functional materials, and specifically relates to a rhodamine 6G-based fluorescent probe molecule for chromium ion detection, a preparation method and its application for chromium ion detection. Background technique [0002] Trivalent chromium is mainly present in glucose tolerance factor and functions as an active ingredient. Current research shows that the chromium in general food is trivalent chromium, and trivalent chromium plays an important role in the synthesis and metabolism of carbohydrates, proteins, fats, nucleic acids and amino acids. The chromium content in the human body is very small, and the total chromium content in adults is 1.7-6mg, which mainly exists in the lungs, muscles, liver, heart, kidney, brain and other organs. Chromium deficiency can lead to impaired glucose tolerance, decreased growth rate and lifespan, and elevated serum cholesterol levels. Studies have confirmed that hexava...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/107
CPCC07D491/107C09K11/06C09K2211/1022C09K2211/1029C09K2211/1088G01N21/33G01N21/643
Inventor 李雪梅周莹魏玉玲赵瑞瑞张俊峰徐济仓
Owner CHINA TOBACCO YUNNAN IND
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