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Fluorescent aptamer probe based on Prussian blue nanoparticles as well as preparation method and application of fluorescent aptamer probe

A nanoparticle, Prussian blue technology, applied in the field of biological nanotechnology sensing, to achieve the effect of improving low stability, low detection limit and strong signal

Active Publication Date: 2020-01-10
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no technology to construct fluorescent probes with Prussian blue nanoparticles and modified FAM fluorophore aptamers

Method used

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  • Fluorescent aptamer probe based on Prussian blue nanoparticles as well as preparation method and application of fluorescent aptamer probe
  • Fluorescent aptamer probe based on Prussian blue nanoparticles as well as preparation method and application of fluorescent aptamer probe
  • Fluorescent aptamer probe based on Prussian blue nanoparticles as well as preparation method and application of fluorescent aptamer probe

Examples

Experimental program
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Embodiment 1

[0048] The preparation method of Prussian blue nanoparticles, the steps are as follows:

[0049] Take 20mL, 1mM K 4 [Fe(CN) 6 ] Aqueous solution, heated to 60℃; 20mL, 1mM Fe(NO 3 ) 3 Aqueous solution; the dripping process lasts for about 10 minutes and keep stirring. After 5 minutes, remove the heat source, keep stirring and cool to room temperature; wash the solution alternately with water and ethanol 3 times, and then vacuum dry to obtain the desired Prussian blue nanoparticles.

[0050] Nano fluorescent probe FAM-Apt Aβ The preparation method of @PBNPs, the steps are as follows:

[0051] Take 10μL of FAM-Apt Aβ (500nM), 9μL PBNPs(1mg.mL -1 ) And 74μL HEPEs buffer (25mM, pH7.6, 100mM NaCl, 1mM MgCl 2 ) Mix, incubate for 60 minutes at 25°C in the dark, add 7 μL BSA (10 μM), and continue to incubate for 60 minutes to obtain the nano fluorescent probe.

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Abstract

The invention discloses a fluorescent aptamer probe based on Prussian blue nanoparticles as well as a preparation method and application of the fluorescent aptamer probe. The fluorescent aptamer probeis composed of Prussian blue nanoparticle complex modified FAM fluorophore aptamers. The preparation method comprises the following steps: incubating the Prussian blue nanoparticles and the aptamersmodified with the FAM fluorophore in an HEPEs buffer solution system in a dark place, and closing by adopting BSA, thereby obtaining the fluorescent aptamer probe. The fluorescent aptamer probe can beused for detecting various markers such as markers of tumor, breast cancer, blood glucose and Alzheimer's disease, has the advantages of strong signal, high specificity, high sensitivity, wide detection concentration range, good biological safety and the like, and is beneficial to popularization and application.

Description

Technical field [0001] The invention relates to a fluorescent aptamer probe, in particular to a fluorescent aptamer probe for marker fluorescence detection constructed based on the fluorescence quenching effect of Prussian blue nanoparticles on FAM fluorophores, and also to a fluorescent aptamer probe The method of detecting various markers through changes in fluorescence signals belongs to the field of bio-nanotechnology sensing. Background technique [0002] In recent years, with the development and research of nanotechnology, fluorescent aptamer sensors based on nanomaterials have received extensive attention. Various nanomaterials, such as gold nanoparticles (Hao W.,Yongxiang W.,Jianyu J.,et al.Goldnanoparticle-based colorimetric and"turn-on"fluorescent probe for mercury(II)ions in aqueous solution[J], Analytical Chemistry, 2008, 80(23): 9021-9028.), CeO 2 (Pautler R.,Kelly E.Y.,Huang P.J.,et al.Attaching DNA to nanoceria:regulatingoxidase activity and fluorescence quenching...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/65C09K11/02G01N21/64
CPCC09K11/02C09K11/06C09K11/65G01N21/6428G01N2021/6432
Inventor 邓春艳陈文兰
Owner CENT SOUTH UNIV
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