Fluorescence detection device

Abstract

The embodiment of the invention discloses a fluorescence detection device, which comprises a light source module, a reaction module and a detection module, wherein the light source module comprises a plurality of monochromatic source components, a plurality of monochromatic source moving tracks, a first driving component and a first control component; the first control component is used for determining a target monochromatic source component from the monochromatic source components based on a selection instruction so as to output a moving instruction to the first driving component, and the first driving component responds to the moving instruction to drive the target monochromatic source component to move to the final position in the corresponding monochromatic source moving track; and the first control component controls the target monochromatic source component to start after the target monochromatic source component moves to the final position so as to output the target exciting light. According to the embodiment of the invention, the plurality of monochromatic light source components are connected with the plurality of sliding rails, the first driving component and the first control component, so that different monochromatic light sources can be selected to provide exciting light with different wavelengths, and fluorescence detection is carried out.

Description

technical field [0001] The invention relates to the technical field of fluorescence detection, in particular to a fluorescence detection device. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction, PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, which can economically and quickly obtain a large amount of DNA of specific fragments. The PCR reaction conditions are temperature, time and number of cycles. Based on the three steps of PCR principle, three temperature points of denaturation-annealing-extension are set. A cycle consists of several steps of high temperature denaturation, low temperature annealing, and suitable temperature extension. The cycle is carried out so that the target DNA can be rapidly amplified. It has the characteristics of strong specificity, high sensitivity, easy operation, and time saving. In recent years, the use of tumor suppressor genes to treat tumors has attracted extensi...

AI Technical Summary

Problems solved by technology

However, traditional PCR real-time quantitative fluorescence detection devices use high-pressure mercury lamps or xenon lamps as excitation light sources, and combine filters or monochromators to provide monochromatic light sources for the detection of single fluorescent dyes. However, if the test needs to use When detecting fluorescent dyes of different colors, the complexity of the fluorescence detection device is greatly increased, and it is difficult to meet its needs

In addition, traditional excitation light sources such as high-pressure mercury lamps and xenon lamps have the disadvantages of short life, large volume, and high cost. At the same time, the monochromatic light source obtained through the aforementioned settings has low power and low energy, which is difficult to meet the basic needs of high-precision detection equipment.

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