Preparation method of fluorescent biosensor of double-cycle and double quenching system based on functionalized graphene quantum dots

A technology of graphene quantum dots and biosensors, applied in the field of biosensors, can solve problems such as complex reaction conditions and expensive enzymes, and achieve the effects of high fluorescence emission intensity, strong proton exchange ability, and accelerated decomposition

Inactive Publication Date: 2019-04-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of enzymes may limit the nucleic acid sequences in the probes as they require specific recognition sites from the probes
In addition, enzymes are expensive and the reaction conditions are complex

Method used

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  • Preparation method of fluorescent biosensor of double-cycle and double quenching system based on functionalized graphene quantum dots
  • Preparation method of fluorescent biosensor of double-cycle and double quenching system based on functionalized graphene quantum dots
  • Preparation method of fluorescent biosensor of double-cycle and double quenching system based on functionalized graphene quantum dots

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Experimental program
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Effect test

Embodiment 1

[0039] A method for preparing a fluorescent biosensor based on a double cycle and double quenching system of functionalized graphene quantum dots, comprising the steps of:

[0040] (1) Weigh raw materials citric acid, histidine and pentaethylene hexamine and mix evenly, wherein the molar ratio of citric acid, histidine and pentaethylene hexamine is 1:1:1, add deionized water and perform ultrasonic dissolution Obtain the reaction solution, put the reaction solution in an oven, and react for 10 hours at an oven temperature of 150° C., and filter to obtain the crude product of pentaethylenehexamine-histidine graphene quantum dot PEHA-GQD-His after the reaction is completed;

[0041] (2) The crude product of pentaethylenehexamine-histidine graphene quantum dot PEHA-GQD-His in step (1) was prepared into a 25 mg / ml aqueous solution and placed in a dialysis bag for dialysis in deionized water to remove For the small molecular substances in the solution, precipitation occurs in the di...

Embodiment 2

[0046] A method for preparing a fluorescent biosensor based on a double cycle and double quenching system of functionalized graphene quantum dots, comprising the steps of:

[0047] (1) Weigh raw materials citric acid, histidine and pentaethylene hexamine and mix evenly, wherein the molar ratio of citric acid, histidine and pentaethylene hexamine is 0.01:0.1:1, add deionized water and perform ultrasonic dissolution Obtain the reaction solution, put the reaction solution in an oven, and react for 2 hours at the temperature of the oven at 200° C. After the reaction, filter to obtain the crude product of pentaethylenehexamine-histidine graphene quantum dot PEHA-GQD-His;

[0048] (2) The crude product of pentaethylenehexamine-histidine graphene quantum dot PEHA-GQD-His in step (1) was prepared into a 100 mg / ml aqueous solution and placed in a dialysis bag for dialysis in deionized water to remove For the small molecular substances in the solution, precipitation occurs in the dialys...

Embodiment 3

[0053] A method for preparing a fluorescent biosensor based on a double cycle and double quenching system of functionalized graphene quantum dots, comprising the steps of:

[0054] (1) Weigh raw materials citric acid, histidine and pentaethylene hexamine and mix evenly, wherein the molar ratio of citric acid, histidine and pentaethylene hexamine is 0.01:0.1:1, add deionized water and perform ultrasonic dissolution Obtain the reaction solution, put the reaction solution in an oven, and react for 0.50h at the temperature of the oven at 250° C., and filter to obtain the crude product of pentaethylenehexamine-histidine graphene quantum dot PEHA-GQD-His after the reaction is completed;

[0055] (2) The crude product of pentaethylenehexamine-histidine graphene quantum dot PEHA-GQD-His in step (1) was prepared into a 200mg / ml aqueous solution, and then placed in a dialysis bag for dialysis in deionized water to remove For the small molecular substances in the solution, precipitation ...

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Abstract

The invention relates to a preparation method of a fluorescent biosensor of a double-cycle and double quenching system based on functionalized graphene quantum dots. The method comprises the followingsteps: weighing raw materials including citric acid, histidine and pentaethylene hexamine at the molar ratio of 0.01:0.1:1 to 1:1:1, uniformly mixing, adding deionized water, then performing ultrasonic dissolution to obtain a reaction solution, placing the reaction solution in an oven, reacting for 0.5-10h at an oven temperature of 150-250 DEG C to directly obtain a crude product of pentaethylenehexamine-histidine graphene quantum dot PEHA-GQD-His after reaction, then obtaining an LDNA-capped pentaethylene hexamine-histidine graphene quantum dot PEHA-GQD-His solution, finally forming a G-quadruplex / DNase, and finally adding 0.5-100[mu]l of H2O2 with the molar concentration of 0.1-10[mu]M to obtain the fluorescent biosensor of the double-cycle and double quenching system.

Description

technical field [0001] The invention relates to a fluorescent biosensor based on a double cycle and double quenching system of functionalized graphene quantum dots, belonging to the technical field of biosensors. Background technique [0002] MicroRNA (miRNA) is a kind of small single-stranded RNA with a length of 18-25 nucleotides, which is produced by DNA transcription and is not translated into protein. It is partially or completely complementary to the 3'UTR region of the target gene through complementary base pairing. , cutting the transcription product of the target gene or inhibiting the translation of the transcription product, thereby playing the role of controlling the expression of the target gene after transcription. The expression of miRNA has tissue and time specificity, and it is an important regulatory molecule that regulates the expression of other functional genes, and plays an important role in various physiological activities, growth and development of or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6825
CPCC12Q1/6825C12Q2563/107
Inventor 李在均李娜娜李瑞怡
Owner JIANGNAN UNIV
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