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Composite fluorescent biosensor, and preparation method and application thereof

A biosensor and composite fluorescence technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., to achieve good selectivity and stability, good market application prospects, industrial production value, and accurate detection effects

Active Publication Date: 2018-01-26
BEIJING INSTITUTE OF TECHNOLOGYGY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the synthesis and functionalization of GQDs have attracted great attention of researchers, but there is little research on the use of their outstanding fluorescence tunability for biological analysis to construct bioluminescent sensors.

Method used

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  • Composite fluorescent biosensor, and preparation method and application thereof
  • Composite fluorescent biosensor, and preparation method and application thereof
  • Composite fluorescent biosensor, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] In a 50mL three-necked flask, mix melamine and formaldehyde solution (concentration: 37%) according to the molar ratio of 1: (3-5), stir mechanically, heat to 60°C in a water bath and react for 20min to obtain prepolymer methylol melamine. Add polyvinyl alcohol as a dispersant in a 250mL three-necked bottle, and dissolve it in 90g of deionized water with mechanical stirring in a water bath. After it is completely dissolved, use acetic acid to adjust the pH value of the system to weak acidity and adjust to the predetermined temperature. Add the prepolymer into the polyvinyl alcohol solution, and stir at the same time, start timing when the system appears white turbidity, stop heating after about 30 minutes of reaction, cool with ice water, and terminate the reaction. The obtained product dispersion system is centrifuged at high speed by a centrifuge to separate the precipitate, remove the supernatant, add deionized water to redisperse, wash and centrifuge three times, and...

Embodiment 2

[0050] In a 50mL three-neck flask, mix melamine and formaldehyde solution (concentration: 37%) according to the molar ratio of 1: (3-5), stir mechanically, heat to 60°C in a water bath and react for 20min to obtain a prepolymer methylolmelamine. Add polyvinyl alcohol as a dispersant in a 250mL three-necked bottle, and dissolve it in 90g of deionized water with mechanical stirring in a water bath. After it is completely dissolved, use acetic acid to adjust the pH value of the system to weak acidity and adjust to the predetermined temperature. Add the prepolymer into the polyvinyl alcohol solution, and stir at the same time, start timing when the system appears white turbidity, stop heating after about 30 minutes of reaction, cool with ice water, and terminate the reaction. The obtained product dispersion system is centrifuged at high speed by a centrifuge to separate the precipitate, remove the supernatant, add deionized water to redisperse, wash and centrifuge three times. The...

Embodiment 3

[0056] In a 50mL three-necked flask, mix melamine and formaldehyde solution (concentration: 37%) according to the molar ratio of 1: (3-5), stir mechanically, heat to 60°C in a water bath and react for 20min to obtain prepolymer methylol melamine. Add polyvinyl alcohol as a dispersant in a 250mL three-necked bottle, and dissolve it in 90g of deionized water with mechanical stirring in a water bath. After it is completely dissolved, use acetic acid to adjust the pH value of the system to weak acidity and adjust to the predetermined temperature. Add the prepolymer into the polyvinyl alcohol solution, and stir at the same time, start timing when the system appears white turbidity, stop heating after about 30 minutes of reaction, cool with ice water, and terminate the reaction. The obtained product dispersion system is centrifuged at high speed by a centrifuge to separate the precipitate, remove the supernatant, add deionized water to redisperse, wash and centrifuge three times. Th...

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Abstract

The invention relates to a composite fluorescent biosensor, and a preparation method and an application thereof. The composite fluorescent biosensor comprises a graphene quantum dot and a carboxymethyl cellulose-chitosan polyelectrolyte microsphere; and the carboxymethyl cellulose-chitosan polyelectrolyte microsphere is a microsphere having a core-shell structure, the core is made of melamine andformaldehyde, and the shell is made of chitosan and carboxymethyl cellulose. The preparation method of the organic-inorganic composite fluorescent biosensor and a fluorescence detection method of target biological polysaccharides are helpful for improving other biological sensors. The composite fluorescent biosensor formed by cellulose ether-based polyelectrolyte microsphere induced graphene quantum dot aggregation has a very good market prospect and very good industrial production values.

Description

technical field [0001] The invention belongs to the technical field of biological polysaccharide and drug detection, and specifically relates to a cellulose ether-based polyelectrolyte microsphere that induces the aggregation of graphene quantum dots to form a composite fluorescent biosensor for realizing accurate detection of chondroitin sulfate and a preparation method thereof. Background technique [0002] Biological polysaccharides are one of the very important components of living organisms. They widely exist in the connective tissues of all organisms, participate in the immune regulation process of organisms and various activities of life cells, and have the functions of regulating blood lipids, anti-inflammation, anti- Tumor and enhance basal immunity and other important roles. Based on the above-mentioned effects of biological polysaccharides on living organisms, it is particularly important to accurately detect the quality control and content of biological polysacch...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 崔萌邵自强李京桐路大勇
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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