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Bovine serum albumin-stabilized copper nano-cluster fluorescence biosensor and preparation method and application thereof

A bovine serum albumin and biosensor technology, which is applied in the field of materials science and analytical chemistry, can solve the problems of few research reports on fluorescent copper nanoclusters, achieve improved sensitivity and detection limit, good stability, and mild reaction conditions Effect

Inactive Publication Date: 2016-06-22
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on fluorescent copper nanoclusters

Method used

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  • Bovine serum albumin-stabilized copper nano-cluster fluorescence biosensor and preparation method and application thereof
  • Bovine serum albumin-stabilized copper nano-cluster fluorescence biosensor and preparation method and application thereof
  • Bovine serum albumin-stabilized copper nano-cluster fluorescence biosensor and preparation method and application thereof

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preparation example Construction

[0029] The preparation method of the copper nano-cluster fluorescent biosensor is as follows: pipette 10-100 μL of fluorescent copper nano-cluster solution and evenly disperse it in 5 mL of triple distilled water.

[0030] The method for preparing the bovine serum albumin-stabilized fluorescent copper nanocluster comprises the following steps:

[0031] (1) Add 1~5mL0.02M copper sulfate solution into 5~10mL15mg / mL bovine serum albumin solution, stir at room temperature for 5min;

[0032] (2) Adjust the pH value of the solution obtained in step (1) to 12 with 1M NaOH solution;

[0033] (3) Put the solution obtained in step (2) at 52-58 ? C Stirring and reacting for 36-60 hours to prepare bovine serum albumin-stabilized fluorescent copper nano-clusters.

[0034] In the step (1), the dosage of 0.02M copper sulfate solution is 1-5 mL; the dosage of 15 mg / mL bovine serum albumin solution is 5-10 mL.

[0035] The pH value of the solution in the step (2) is 12.

[0036] In the des...

Embodiment 1

[0039] The bovine serum albumin-stabilized copper nano-cluster fluorescent biosensor of the invention uses the bovine serum albumin-stabilized copper nano-cluster as a fluorescent probe to construct the biosensor.

[0040] The preparation method of the copper nanocluster fluorescent biosensor is as follows: pipette 10 μL of fluorescent copper nanocluster solution and evenly disperse it in 5 mL of triple distilled water.

[0041] The method for preparing the bovine serum albumin-stabilized fluorescent copper nanocluster comprises the following steps:

[0042] (1) Add 1mL of 0.02M copper sulfate solution to 5mL of 15mg / mL bovine serum albumin solution, and stir at room temperature for 5min;

[0043] (2) Adjust the pH value of the solution obtained in step (1) to 12 with 1M NaOH solution;

[0044] (3) the solution obtained in the step (2) at 52 ? C stirred and reacted for 60 hours to prepare bovine serum albumin-stabilized fluorescent copper nanoclusters.

Embodiment 2

[0046] The bovine serum albumin-stabilized copper nano-cluster fluorescent biosensor of the invention uses the bovine serum albumin-stabilized copper nano-cluster as a fluorescent probe to construct the biosensor.

[0047] The preparation method of the copper nanocluster fluorescent biosensor is as follows: pipette 50 μL of fluorescent copper nanocluster solution and evenly disperse it in 5 mL of triple distilled water.

[0048] The method for preparing the bovine serum albumin-stabilized fluorescent copper nanocluster comprises the following steps:

[0049] (1) Add 3mL0.02M copper sulfate solution to 7mL15mg / mL bovine serum albumin solution, stir at room temperature for 5min;

[0050] (2) Adjust the pH value of the solution obtained in step (1) to 12 with 1M NaOH solution;

[0051] (3) the solution obtained in the step (2) at 55 ? C stirred and reacted for 48 hours to prepare bovine serum albumin-stabilized fluorescent copper nanoclusters.

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Abstract

The invention discloses a bovine serum albumin-stabilized copper nano-cluster fluorescence biosensor and a preparation method and application thereof. The preparation method mainly comprises the step of preparation of a bovine serum albumin-stabilized copper nano-cluster; the fluorescence biosensor is used for sensitivity detection on hydrogen peroxide. According to a preparation method of the bovine serum albumin-stabilized copper nano-cluster, the copper nano-cluster with the fluorescence property is prepared by taking bovine serum albumin as a stabilizing agent and taking a copper sulphate solution as a copper source. The preparation method of the fluorescence copper nano-cluster is simple and low in cost and does not need any reducing agents, the prepared fluorescence copper nano-cluster has the advantages of being low in toxicity, good in light stability, high in quantum yield, good in biocompatibility and the like, and high sensitivity determination on hydrogen peroxide can be achieved through the copper nano-cluster. The copper nano-cluster has the wide application prospect in the fields of food safety, biochemical analysis, cell imaging, clinical diagnosis and the like by serving as a fluorescence probe.

Description

technical field [0001] The invention relates to the fields of material science and analytical chemistry, in particular to a bovine serum albumin-stabilized copper nano-cluster fluorescent biosensor and its preparation method and application. Background technique [0002] As a member of the reactive oxygen species (ROS) family, hydrogen peroxide is a common chemical substance widely present in food, medicine, blood and industrial fields. Recent studies have shown that hydrogen peroxide produced in mammalian cells plays a key role in regulating various physiological responses such as cell proliferation, differentiation and migration. Moreover, the concentration of hydrogen peroxide in the human body can be used as an important indicator to evaluate the different stages of diseases such as Parkinson's disease, cancer and Alzheimer's disease. Hydrogen peroxide is often used as a cleaning agent and disinfectant in industrial production processes, such as packaging and processing...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/643G01N2021/6432
Inventor 王海波陈颖李阳张红定刘彦明
Owner XINYANG NORMAL UNIVERSITY
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