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Detection reagent and test strip for creatinine

A detection reagent and detection test strip technology, applied in the field of creatinine detection, can solve the problem of insufficient detection speed, and achieve the effects of rapid and accurate detection, rapid detection and accurate detection

Active Publication Date: 2017-05-10
复星诊断科技(长沙)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the above existing technologies, the detection accuracy needs to be improved, and the detection is not fast enough

Method used

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  • Detection reagent and test strip for creatinine
  • Detection reagent and test strip for creatinine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Step 1: Paste double-sided adhesive tape 40 on the reactive base layer 30 made of PET with a certain hardness, and punch holes for later use.

[0056] Step 2: tear off the double-sided adhesive tape on the reaction base layer 40, and paste the reaction film 50 on the double-sided tape 40 of the PET board. The reaction film 50 needs to completely cover the hole 31 on the PET substrate.

[0057] Step 3. Prepare the detection reagent according to the ratio: 12KU / L creatinase, 12KU / L creatine hydrolase, 9KU / L sarcosine oxidase, 15KU / L horseradish peroxidase, 5KU / L ascorbate oxidase, 0.20g / L 4-aminoantipyrine, 0.20g / L chromogenic substance, 0.15mmol / L (pH 6.0) PBS buffer, 1.0g / L g / L seaweed Sugar, 0.15g / L BSA. Add the prepared detection reagent dropwise into the wells of the reaction membrane plate with a dispenser, the weight of each hole is 6mg, and put the membrane plate after dripping into the drying tunnel for drying. The drying temperature is 37°C and the drying time...

Embodiment 2

[0061] In this example, the detection reagent contains 18KU / L creatinase, 20KU / L creatine hydrolase, 12KU / L sarcosine oxidase, 20KU / L horseradish peroxidase, 8KU / L ascorbate oxidase , 0.35g / L 4-aminoantipyrine, 0.30g / L chromogenic substance, 0.25mmol / L (pH 8.0) PBS buffer, 1.3g / L g / L trehalose, 0.35g / L BSA, other are all the same as embodiment 1.

Embodiment 3

[0063] In this example, the detection reagent contains 12KU / L creatinase, 20KU / L creatine hydrolase, 9KU / L sarcosine oxidase, 15KU / L horseradish peroxidase, 8KU / L ascorbate oxidase , 0.20g / L 4-aminoantipyrine, 0.25g / L chromogenic substance, 0.20mmol / L (pH is 7) PBS buffer, 1.0g / L g / L trehalose, 0.35g / L BSA, other are all the same as embodiment 1.

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Abstract

The invention discloses a detection reagent and a test strip for creatinine. Each liter of the detection reagent contains 12-18 KU of creatininase, 12-20 KU of creatinase, 9-12 KU of sarcosine oxidase, 15-20 KU of horseradish peroxidase, 5-8 KU of ascorbic acid oxidase, 0.20-0.35 g of 4-aminoantipyrine and 0.20-0.30 g of color developing matter. The detection reagent contains various specific enzymes for specific proportioning, and can achieve the purposes of rapid detection and more accurate detection. The test strip comprises a reaction base layer, a reaction layer, a blood filter layer and a hydrophilic layer which are stacked to form a siphon system, thereby further guaranteeing rapid detection and more accurate detection.

Description

technical field [0001] The invention relates to the technical field of creatinine detection, in particular to a creatinine detection reagent and a creatinine detection test paper. Background technique [0002] Currently, methods for detecting the presence or amount of a certain component in blood include liquid reagent testing and dry chemical test strip testing. Dry chemical test paper has the characteristics of convenient use, simple operation and quick results. [0003] According to the flow path of the liquid sample on the dry chemical test paper, the dry chemical test paper can be divided into various types, and the vertical vertical detection test paper is a kind of dry chemical test paper. The vertical vertical detection test paper includes stacking the carriers with the reaction reagents on top of each other, the liquid sample flows through each reaction reagent layer from top to bottom, and finally displays the detection results. For example, U.S. Patent No. 4,816...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/70
CPCG01N33/70
Inventor 彭勇李文韬李小慧
Owner 复星诊断科技(长沙)有限公司
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